Eubacterium ventriosum ATCC 27560

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; Eubacterium ventriosum

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2776 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5Z437|A5Z437_9FIRM Conserved domain protein OS=Eubacterium ventriosum ATCC 27560 OX=411463 GN=EUBVEN_00443 PE=4 SV=1
MM1 pKa = 7.55SFTIDD6 pKa = 3.34TAQEE10 pKa = 3.93QPINLAPQTLYY21 pKa = 11.29EE22 pKa = 4.08EE23 pKa = 5.13VIQNVWFLLSSLEE36 pKa = 3.76YY37 pKa = 10.36DD38 pKa = 2.92IPLNRR43 pKa = 11.84EE44 pKa = 3.5FGLNAAYY51 pKa = 9.34IDD53 pKa = 4.18KK54 pKa = 10.55PITTATALATADD66 pKa = 3.43IYY68 pKa = 11.76DD69 pKa = 4.34KK70 pKa = 10.85IGEE73 pKa = 4.17YY74 pKa = 10.03EE75 pKa = 3.88PRR77 pKa = 11.84AEE79 pKa = 3.99IVSIDD84 pKa = 3.38FTTDD88 pKa = 3.07YY89 pKa = 11.09EE90 pKa = 4.53RR91 pKa = 11.84GILKK95 pKa = 10.12PKK97 pKa = 10.67VEE99 pKa = 4.09VEE101 pKa = 4.19VNGEE105 pKa = 3.59YY106 pKa = 10.59DD107 pKa = 3.65EE108 pKa = 5.07YY109 pKa = 11.54DD110 pKa = 3.42EE111 pKa = 5.62EE112 pKa = 4.42YY113 pKa = 10.97TEE115 pKa = 4.26

Molecular weight: 13.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5Z7F4|A5Z7F4_9FIRM PemK-like protein OS=Eubacterium ventriosum ATCC 27560 OX=411463 GN=EUBVEN_01640 PE=3 SV=1
MM1 pKa = 6.6KK2 pKa = 10.27QKK4 pKa = 10.2RR5 pKa = 11.84RR6 pKa = 11.84CLIMKK11 pKa = 7.64MTFQPKK17 pKa = 8.95KK18 pKa = 7.58RR19 pKa = 11.84QRR21 pKa = 11.84SKK23 pKa = 9.07VHH25 pKa = 5.95GFRR28 pKa = 11.84KK29 pKa = 9.97RR30 pKa = 11.84MSTAGGRR37 pKa = 11.84KK38 pKa = 8.72VLASRR43 pKa = 11.84RR44 pKa = 11.84AKK46 pKa = 10.13GRR48 pKa = 11.84KK49 pKa = 8.85KK50 pKa = 10.63LSAA53 pKa = 3.95

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2776

0

2776

829464

29

2219

298.8

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.448 ± 0.049

1.389 ± 0.023

5.737 ± 0.041

7.192 ± 0.061

4.125 ± 0.037

6.627 ± 0.042

1.46 ± 0.019

8.242 ± 0.053

8.76 ± 0.059

7.906 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.971 ± 0.028

5.675 ± 0.042

2.905 ± 0.03

2.788 ± 0.027

3.532 ± 0.036

6.075 ± 0.045

5.844 ± 0.061

7.007 ± 0.042

0.882 ± 0.018

4.435 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski