Clostridium sp. CAG:1193

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5J7M4|R5J7M4_9CLOT Stage 0 sporulation protein A homolog OS=Clostridium sp. CAG:1193 OX=1262771 GN=BN475_01028 PE=4 SV=1
MM1 pKa = 7.37FKK3 pKa = 10.39VDD5 pKa = 3.51KK6 pKa = 9.64EE7 pKa = 4.11KK8 pKa = 11.15CIGCGACVGTCDD20 pKa = 4.93NVFDD24 pKa = 5.67FDD26 pKa = 5.48DD27 pKa = 5.34DD28 pKa = 3.6NLAYY32 pKa = 10.61VKK34 pKa = 10.28NQPNNEE40 pKa = 4.04NEE42 pKa = 4.06NLAIEE47 pKa = 4.56AMEE50 pKa = 4.28NCPTNAIEE58 pKa = 5.36KK59 pKa = 9.47EE60 pKa = 4.13

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5J7Z0|R5J7Z0_9CLOT 30S ribosomal protein S20 OS=Clostridium sp. CAG:1193 OX=1262771 GN=rpsT PE=3 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84KK3 pKa = 9.46VEE5 pKa = 4.2AYY7 pKa = 10.6VNGVGPARR15 pKa = 11.84DD16 pKa = 3.18TALRR20 pKa = 11.84SLQTGGLEE28 pKa = 4.01VTAMSDD34 pKa = 3.37VTPIPHH40 pKa = 6.45NGCRR44 pKa = 11.84PPKK47 pKa = 9.98RR48 pKa = 11.84PRR50 pKa = 11.84GG51 pKa = 3.65

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1080

0

1080

309167

29

2727

286.3

32.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.176 ± 0.069

1.114 ± 0.033

6.244 ± 0.064

7.069 ± 0.075

4.125 ± 0.056

5.332 ± 0.082

1.202 ± 0.03

10.394 ± 0.105

10.134 ± 0.089

9.011 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.033

7.637 ± 0.093

2.361 ± 0.039

1.592 ± 0.033

3.026 ± 0.046

6.566 ± 0.07

5.491 ± 0.088

6.294 ± 0.059

0.513 ± 0.021

5.215 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski