Midway nyavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Nyamiviridae; Nyavirus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4NFL4|C4NFL4_9MONO Nucleocapsid OS=Midway nyavirus OX=644609 PE=4 SV=1
MM1 pKa = 7.2NPEE4 pKa = 4.23ANQEE8 pKa = 4.56GIDD11 pKa = 3.89STEE14 pKa = 3.87PTAVSATGYY23 pKa = 9.89SVARR27 pKa = 11.84HH28 pKa = 6.25RR29 pKa = 11.84DD30 pKa = 3.23QCPPEE35 pKa = 4.37EE36 pKa = 4.39NSDD39 pKa = 3.66SQEE42 pKa = 3.94EE43 pKa = 4.13PKK45 pKa = 10.83EE46 pKa = 4.04RR47 pKa = 11.84GSEE50 pKa = 4.19EE51 pKa = 4.14EE52 pKa = 3.79ASTWAVEE59 pKa = 4.94TYY61 pKa = 10.43QQDD64 pKa = 3.53QAHH67 pKa = 6.6EE68 pKa = 4.27KK69 pKa = 10.15QGAEE73 pKa = 4.2GPPCASRR80 pKa = 11.84SDD82 pKa = 3.92PEE84 pKa = 3.91AAGPEE89 pKa = 4.24EE90 pKa = 4.44EE91 pKa = 4.45VSEE94 pKa = 4.12EE95 pKa = 4.53DD96 pKa = 3.66EE97 pKa = 5.17EE98 pKa = 4.35EE99 pKa = 5.09DD100 pKa = 3.49EE101 pKa = 5.49DD102 pKa = 4.36EE103 pKa = 4.4EE104 pKa = 5.51SEE106 pKa = 4.12IDD108 pKa = 3.34SDD110 pKa = 4.5EE111 pKa = 4.35IFISTSSEE119 pKa = 3.89SDD121 pKa = 3.04SDD123 pKa = 4.25SSTEE127 pKa = 3.9TADD130 pKa = 5.17LDD132 pKa = 3.54KK133 pKa = 11.3CMQARR138 pKa = 11.84FVQWQSNILQLLRR151 pKa = 11.84SDD153 pKa = 3.23ARR155 pKa = 11.84KK156 pKa = 10.18DD157 pKa = 3.42RR158 pKa = 11.84EE159 pKa = 4.0EE160 pKa = 4.16GSRR163 pKa = 11.84AIALTYY169 pKa = 9.91RR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 11.84RR173 pKa = 11.84EE174 pKa = 3.73QGGSRR179 pKa = 11.84FYY181 pKa = 10.9SWQNLVILEE190 pKa = 4.35SEE192 pKa = 4.71LARR195 pKa = 11.84AAPNVLTLRR204 pKa = 11.84SPATPEE210 pKa = 3.5ASRR213 pKa = 11.84WDD215 pKa = 3.55AVLDD219 pKa = 3.72EE220 pKa = 4.4LRR222 pKa = 11.84GLKK225 pKa = 10.61LEE227 pKa = 4.85DD228 pKa = 3.46EE229 pKa = 4.67AA230 pKa = 6.54

Molecular weight: 25.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4NFL2|C4NFL2_9MONO ORF III OS=Midway nyavirus OX=644609 PE=4 SV=1
MM1 pKa = 7.19QVRR4 pKa = 11.84AKK6 pKa = 9.74EE7 pKa = 3.81ASVWYY12 pKa = 8.73QVKK15 pKa = 10.22VKK17 pKa = 10.1TPMRR21 pKa = 11.84DD22 pKa = 2.71VSLAVPVCRR31 pKa = 11.84VPRR34 pKa = 11.84KK35 pKa = 10.47GEE37 pKa = 3.82FEE39 pKa = 3.92QRR41 pKa = 11.84PDD43 pKa = 3.22PKK45 pKa = 10.86VFKK48 pKa = 10.32DD49 pKa = 3.63SKK51 pKa = 10.73ACIFIHH57 pKa = 6.79DD58 pKa = 4.48RR59 pKa = 11.84KK60 pKa = 9.94PYY62 pKa = 10.66LRR64 pKa = 11.84IEE66 pKa = 4.66LDD68 pKa = 3.33PEE70 pKa = 4.07RR71 pKa = 11.84ASGQKK76 pKa = 10.42DD77 pKa = 3.15KK78 pKa = 11.45TKK80 pKa = 10.18IPQSQVVCLAPYY92 pKa = 8.4GWKK95 pKa = 10.21VRR97 pKa = 11.84DD98 pKa = 4.25FAAHH102 pKa = 6.77PLQVRR107 pKa = 11.84YY108 pKa = 8.96EE109 pKa = 3.92QVVFKK114 pKa = 10.07EE115 pKa = 4.22PCAPLLDD122 pKa = 4.18EE123 pKa = 5.24SNPFLFSQEE132 pKa = 3.76

Molecular weight: 15.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3689

132

1935

614.8

69.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.102 ± 0.689

2.087 ± 0.45

4.852 ± 0.895

8.349 ± 2.213

2.602 ± 0.477

5.855 ± 0.438

2.494 ± 0.474

4.229 ± 0.349

6.099 ± 1.109

12.009 ± 1.976

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.898 ± 0.187

2.873 ± 0.241

5.34 ± 0.425

4.69 ± 0.351

6.912 ± 1.006

7.671 ± 0.686

5.801 ± 0.81

4.608 ± 0.629

1.654 ± 0.329

2.873 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski