Simian immunodeficiency virus SIV-mnd 2

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Simian immunodeficiency virus; Simian immunodeficiency virus - mnd

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8AII4|Q8AII4_SIV Envelope glycoprotein gp160 OS=Simian immunodeficiency virus SIV-mnd 2 OX=159122 GN=env PE=4 SV=1
MM1 pKa = 7.45GGKK4 pKa = 9.25SSKK7 pKa = 8.26QQEE10 pKa = 4.34EE11 pKa = 5.26KK12 pKa = 10.16YY13 pKa = 10.44LKK15 pKa = 9.99YY16 pKa = 10.16YY17 pKa = 10.39KK18 pKa = 10.74AMRR21 pKa = 11.84RR22 pKa = 11.84GYY24 pKa = 9.97GAEE27 pKa = 4.12GTNGDD32 pKa = 4.11YY33 pKa = 10.62QQLHH37 pKa = 6.15ASEE40 pKa = 4.83PLLGALSTSQEE51 pKa = 3.96EE52 pKa = 4.15FDD54 pKa = 4.78RR55 pKa = 11.84EE56 pKa = 4.29QKK58 pKa = 10.68SSSTDD63 pKa = 3.27EE64 pKa = 4.15EE65 pKa = 4.89EE66 pKa = 4.11EE67 pKa = 4.18TGFPVYY73 pKa = 9.07PQCPVRR79 pKa = 11.84EE80 pKa = 4.21PTYY83 pKa = 10.99KK84 pKa = 10.73DD85 pKa = 3.77LVDD88 pKa = 3.72MPHH91 pKa = 6.39FLKK94 pKa = 10.83EE95 pKa = 3.94KK96 pKa = 10.59GGLEE100 pKa = 4.56GIWHH104 pKa = 5.85SKK106 pKa = 8.98RR107 pKa = 11.84RR108 pKa = 11.84EE109 pKa = 4.06EE110 pKa = 5.02ILDD113 pKa = 4.22LYY115 pKa = 10.06AQNEE119 pKa = 4.25WGFIPTWQSYY129 pKa = 8.92TDD131 pKa = 4.01GPGIRR136 pKa = 11.84YY137 pKa = 7.55PKK139 pKa = 9.6TFRR142 pKa = 11.84FLFKK146 pKa = 10.07LCPVAVPPDD155 pKa = 3.6QEE157 pKa = 4.69NNEE160 pKa = 4.4CNKK163 pKa = 10.16LLQSSQLGIQEE174 pKa = 4.09EE175 pKa = 4.7SLGRR179 pKa = 11.84EE180 pKa = 3.85ASVEE184 pKa = 4.14VV185 pKa = 3.72

Molecular weight: 21.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8AII6|Q8AII6_SIV Protein Vpr OS=Simian immunodeficiency virus SIV-mnd 2 OX=159122 GN=vpr PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 5.12AGKK5 pKa = 10.27AVSDD9 pKa = 3.85KK10 pKa = 11.35KK11 pKa = 11.06EE12 pKa = 4.19GDD14 pKa = 3.46VTPYY18 pKa = 11.27DD19 pKa = 3.73PFRR22 pKa = 11.84DD23 pKa = 3.36RR24 pKa = 11.84TTPCDD29 pKa = 2.78TCYY32 pKa = 10.66CKK34 pKa = 10.38RR35 pKa = 11.84CCYY38 pKa = 10.1HH39 pKa = 6.59CQLCFLQKK47 pKa = 10.81ALGVHH52 pKa = 4.77YY53 pKa = 10.02HH54 pKa = 7.11AYY56 pKa = 9.32RR57 pKa = 11.84NRR59 pKa = 11.84RR60 pKa = 11.84SRR62 pKa = 11.84QRR64 pKa = 11.84LLEE67 pKa = 4.52KK68 pKa = 10.08ISEE71 pKa = 4.24DD72 pKa = 3.35SRR74 pKa = 11.84VIALFLMSSRR84 pKa = 11.84EE85 pKa = 4.24TISVPTTSNSQAKK98 pKa = 9.58KK99 pKa = 8.96EE100 pKa = 4.35KK101 pKa = 10.37KK102 pKa = 10.47SPTQKK107 pKa = 10.39NRR109 pKa = 11.84VPNQTAPKK117 pKa = 9.87

Molecular weight: 13.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

3107

99

1010

388.4

44.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.504 ± 0.346

2.446 ± 0.508

4.152 ± 0.268

7.467 ± 0.777

2.671 ± 0.169

7.21 ± 0.361

2.414 ± 0.451

5.729 ± 0.627

7.95 ± 0.506

8.368 ± 0.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.06 ± 0.498

4.248 ± 0.4

5.793 ± 0.735

5.536 ± 0.375

5.053 ± 0.6

4.957 ± 0.624

6.083 ± 1.053

6.019 ± 0.883

2.768 ± 0.398

3.573 ± 0.498
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski