Proteome-pI 2.0 - Proteome Isoelectric Point Database

Mycobacterium phage Ogopogo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A2P1CHS9|A0A2P1CHS9_9CAUD Uncharacterized protein OS=Mycobacterium phage Ogopogo OX=2099640 GN=90 PE=4 SV=1

MM1 pKa = 7.19NPEE4 pKa = 3.56LRR6 pKa = 11.84AVLTEE11 pKa = 4.1ALAGHH16 pKa = 5.59QPEE19 pKa = 4.6NYY21 pKa = 9.94GFNCSGCDD29 pKa = 3.34WEE31 pKa = 4.49PANPAVTDD39 pKa = 3.68AAEE42 pKa = 4.29FAAHH46 pKa = 6.3QLDD49 pKa = 4.17VLDD52 pKa = 4.24TAPGVAVIQLPEE64 pKa = 4.17PYY66 pKa = 8.95FVHH69 pKa = 6.99SATDD73 pKa = 3.58DD74 pKa = 3.66VNGYY78 pKa = 10.69ADD80 pKa = 3.33WEE82 pKa = 4.5YY83 pKa = 10.42PHH85 pKa = 7.59GSIATTDD92 pKa = 4.07DD93 pKa = 3.49GTIMWGKK100 pKa = 7.8WHH102 pKa = 6.46ITDD105 pKa = 3.84PEE107 pKa = 4.21KK108 pKa = 11.18VRR110 pKa = 11.84AAAAALLAAAAAAVVAAGEE129 pKa = 4.21EE130 pKa = 4.24AA131 pKa = 3.69

Molecular weight: 13.77 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A2P1CHU4|A0A2P1CHU4_9CAUD Polypeptide N-acetylgalactosaminyl transferase OS=Mycobacterium phage Ogopogo OX=2099640 GN=105 PE=4 SV=1

MM1 pKa = 7.25NRR3 pKa = 11.84RR4 pKa = 11.84EE5 pKa = 4.03IEE7 pKa = 3.92DD8 pKa = 3.69LRR10 pKa = 11.84TKK12 pKa = 10.4KK13 pKa = 9.63IHH15 pKa = 5.45QRR17 pKa = 11.84LIKK20 pKa = 10.18GRR22 pKa = 11.84YY23 pKa = 7.08TIGLAPNGYY32 pKa = 9.8SLTQHH37 pKa = 5.93ACEE40 pKa = 3.76RR41 pKa = 11.84MIARR45 pKa = 11.84RR46 pKa = 11.84IHH48 pKa = 6.14MDD50 pKa = 2.6WVMDD54 pKa = 4.35ALDD57 pKa = 3.91SPARR61 pKa = 11.84PSRR64 pKa = 11.84DD65 pKa = 2.85GAVKK69 pKa = 10.33HH70 pKa = 6.15VGLSATVVVNHH81 pKa = 5.45PMKK84 pKa = 10.66RR85 pKa = 11.84IVTVGYY91 pKa = 10.82GLFNNPTAA99 pKa = 4.35

Molecular weight: 11.22 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
110	0	110	18260	30	1174	166.0	18.18

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
10.548 ± 0.453	1.194 ± 0.164	6.577 ± 0.201	5.986 ± 0.358	2.766 ± 0.145	8.762 ± 0.713	2.306 ± 0.192	4.551 ± 0.197	3.401 ± 0.18	7.24 ± 0.273

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.355 ± 0.13	3.352 ± 0.156	6.172 ± 0.169	3.456 ± 0.21	6.857 ± 0.44	5.778 ± 0.249	6.774 ± 0.297	6.95 ± 0.258	2.322 ± 0.164	2.656 ± 0.154

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski