Burkholderia phage vB_BceS_KL1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kilunavirus; Burkholderia virus KL1

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6NMA4|I6NMA4_9CAUD Tail protein OS=Burkholderia phage vB_BceS_KL1 OX=1132026 GN=KL1_00018 PE=4 SV=1
MM1 pKa = 7.11ATKK4 pKa = 10.32KK5 pKa = 10.62VIYY8 pKa = 8.21FTAGINATSGEE19 pKa = 4.13LADD22 pKa = 3.75IAKK25 pKa = 10.21LNAAAEE31 pKa = 4.13PQYY34 pKa = 11.23EE35 pKa = 4.34VIVANGAANAKK46 pKa = 10.0YY47 pKa = 10.82GEE49 pKa = 4.4TNRR52 pKa = 11.84LIPSDD57 pKa = 4.27FVAGTIPGIYY67 pKa = 10.44GEE69 pKa = 4.63IDD71 pKa = 3.87EE72 pKa = 5.41IDD74 pKa = 4.14PDD76 pKa = 4.33NIPNQALTDD85 pKa = 3.63TQTIVNDD92 pKa = 3.58GDD94 pKa = 3.9TLEE97 pKa = 4.32VTTGGDD103 pKa = 3.22PAGTVTFAVVDD114 pKa = 4.36GVLTGTFVAAEE125 pKa = 4.02

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6NRF8|I6NRF8_9CAUD Uncharacterized protein OS=Burkholderia phage vB_BceS_KL1 OX=1132026 GN=KL1_00053 PE=4 SV=1
MM1 pKa = 7.5IKK3 pKa = 9.82IEE5 pKa = 4.76SNVPMPKK12 pKa = 9.94LRR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 10.19AGAIPANVAKK26 pKa = 10.74AIADD30 pKa = 3.52YY31 pKa = 10.69KK32 pKa = 10.13VAYY35 pKa = 8.79KK36 pKa = 10.39ALHH39 pKa = 6.03GVSSLDD45 pKa = 3.19VRR47 pKa = 11.84YY48 pKa = 9.66EE49 pKa = 3.46NGYY52 pKa = 9.99IYY54 pKa = 10.9VEE56 pKa = 4.04GHH58 pKa = 6.58CGVSLQIFRR67 pKa = 11.84SRR69 pKa = 11.84IKK71 pKa = 10.0QLQYY75 pKa = 11.03RR76 pKa = 11.84KK77 pKa = 10.29GG78 pKa = 3.38

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13726

58

1272

249.6

27.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.54 ± 0.668

0.94 ± 0.14

5.719 ± 0.214

5.785 ± 0.337

3.876 ± 0.183

7.744 ± 0.294

1.333 ± 0.167

5.42 ± 0.205

5.464 ± 0.348

7.832 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.12 ± 0.158

4.743 ± 0.215

4.896 ± 0.321

4.24 ± 0.487

4.961 ± 0.229

5.712 ± 0.243

6.433 ± 0.342

6.542 ± 0.202

1.413 ± 0.141

3.286 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski