Wuhan Insect virus 6

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus; Wuhan 6 insect cytorhabdovirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KXN7|A0A0B5KXN7_9RHAB 4b protein OS=Wuhan Insect virus 6 OX=1608111 GN=4b PE=4 SV=1
MM1 pKa = 7.65ASSSDD6 pKa = 3.5VPNYY10 pKa = 10.77SDD12 pKa = 4.2VGEE15 pKa = 4.49PLDD18 pKa = 3.83NLNYY22 pKa = 10.41TLFNDD27 pKa = 5.29DD28 pKa = 5.83DD29 pKa = 4.68DD30 pKa = 6.87LEE32 pKa = 6.4DD33 pKa = 5.35DD34 pKa = 4.34SPIVCTTTIPVSSSEE49 pKa = 4.4VVVSTHH55 pKa = 6.76KK56 pKa = 10.58EE57 pKa = 4.0SPNLKK62 pKa = 10.19DD63 pKa = 5.89ALDD66 pKa = 4.21EE67 pKa = 4.18LQNMCMEE74 pKa = 4.52KK75 pKa = 10.78GIVCTLAMEE84 pKa = 4.57NQVKK88 pKa = 9.64MISSSEE94 pKa = 4.11EE95 pKa = 3.65IYY97 pKa = 10.55KK98 pKa = 10.35SHH100 pKa = 6.1LTWYY104 pKa = 10.37LRR106 pKa = 11.84GIAMANNTQLLPSIHH121 pKa = 7.34DD122 pKa = 3.97SIGVLKK128 pKa = 10.98SEE130 pKa = 4.65IKK132 pKa = 9.26FTQLSTNNLNNASAKK147 pKa = 9.39LTGAVQEE154 pKa = 4.03ITRR157 pKa = 11.84EE158 pKa = 3.95IRR160 pKa = 11.84TSTEE164 pKa = 3.75MINDD168 pKa = 3.35NFKK171 pKa = 10.05TVLLQISEE179 pKa = 4.32NQVLPSPNSDD189 pKa = 3.49KK190 pKa = 11.49EE191 pKa = 3.64EE192 pKa = 4.0STYY195 pKa = 11.5VKK197 pKa = 10.32VDD199 pKa = 4.42PIYY202 pKa = 10.88QSDD205 pKa = 4.12TVVTSQEE212 pKa = 4.14FTQSEE217 pKa = 4.6SSPDD221 pKa = 3.35AKK223 pKa = 10.69FSVADD228 pKa = 3.52KK229 pKa = 11.42SDD231 pKa = 3.6FNKK234 pKa = 9.93MKK236 pKa = 10.52SAFLISLGMDD246 pKa = 3.77PDD248 pKa = 4.36LLRR251 pKa = 11.84DD252 pKa = 4.16LPDD255 pKa = 4.14GDD257 pKa = 5.57LDD259 pKa = 5.85LFLSDD264 pKa = 4.41NDD266 pKa = 3.63FKK268 pKa = 11.21EE269 pKa = 4.18FRR271 pKa = 11.84SMKK274 pKa = 9.0LTNALKK280 pKa = 10.58RR281 pKa = 11.84EE282 pKa = 4.0LLQTLMKK289 pKa = 10.52EE290 pKa = 4.59FEE292 pKa = 4.39TKK294 pKa = 10.6LDD296 pKa = 4.12DD297 pKa = 4.51GLL299 pKa = 5.17

Molecular weight: 33.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KXN7|A0A0B5KXN7_9RHAB 4b protein OS=Wuhan Insect virus 6 OX=1608111 GN=4b PE=4 SV=1
MM1 pKa = 7.53SLTFRR6 pKa = 11.84DD7 pKa = 3.81TVKK10 pKa = 11.06YY11 pKa = 9.84EE12 pKa = 4.03LVVSEE17 pKa = 4.17NGGVVSLTKK26 pKa = 10.25RR27 pKa = 11.84LNFFRR32 pKa = 11.84EE33 pKa = 4.21WMTKK37 pKa = 8.71GHH39 pKa = 6.69DD40 pKa = 3.31NVRR43 pKa = 11.84IKK45 pKa = 11.14NLIVKK50 pKa = 8.7YY51 pKa = 10.18NSRR54 pKa = 11.84AGPLGSGTIITRR66 pKa = 11.84VRR68 pKa = 11.84DD69 pKa = 3.51NRR71 pKa = 11.84IDD73 pKa = 3.62SDD75 pKa = 4.03LSEE78 pKa = 5.17SSDD81 pKa = 3.41NTLCTAEE88 pKa = 4.44FPVSEE93 pKa = 4.33SVILRR98 pKa = 11.84WEE100 pKa = 4.53SNVWLYY106 pKa = 11.13KK107 pKa = 10.63GDD109 pKa = 4.15LNNVDD114 pKa = 3.97NPPLIFEE121 pKa = 4.87IDD123 pKa = 3.51LVACNMTLGNSVGQFAITCEE143 pKa = 3.98MAFSKK148 pKa = 11.18VMDD151 pKa = 4.65KK152 pKa = 10.52IRR154 pKa = 11.84YY155 pKa = 7.42KK156 pKa = 10.91NPSAKK161 pKa = 10.55LLDD164 pKa = 3.73TSSVKK169 pKa = 10.49GKK171 pKa = 10.2NIEE174 pKa = 4.73HH175 pKa = 5.98ITKK178 pKa = 10.32FSLTDD183 pKa = 3.09KK184 pKa = 10.65GLEE187 pKa = 3.88NGAQLRR193 pKa = 11.84ARR195 pKa = 11.84QTTHH199 pKa = 6.79RR200 pKa = 11.84ADD202 pKa = 5.16GIRR205 pKa = 11.84KK206 pKa = 7.99TIDD209 pKa = 3.22PGLSSNKK216 pKa = 9.4EE217 pKa = 4.01VLDD220 pKa = 4.35SIATKK225 pKa = 10.32RR226 pKa = 11.84ALGYY230 pKa = 10.81HH231 pKa = 6.8PDD233 pKa = 3.51KK234 pKa = 11.41

Molecular weight: 26.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3805

173

2079

634.2

72.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.047 ± 0.692

1.866 ± 0.325

6.702 ± 0.382

5.256 ± 0.729

3.233 ± 0.241

5.309 ± 0.37

2.102 ± 0.225

6.649 ± 0.26

6.57 ± 0.614

10.066 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.679 ± 0.346

5.23 ± 0.519

4.258 ± 0.243

2.444 ± 0.269

5.361 ± 0.778

8.988 ± 0.351

6.439 ± 0.188

6.439 ± 0.137

1.603 ± 0.276

3.758 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski