Synechococcus phage ACG-2014b

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Nereusvirus; Synechococcus virus ACG2014bSyn7803C61

Average proteome isoelectric point is 5.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 215 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3FTG3|A0A0E3FTG3_9CAUD Uncharacterized protein OS=Synechococcus phage ACG-2014b OX=1493508 GN=Syn7803C100_93 PE=4 SV=1
MM1 pKa = 7.77AKK3 pKa = 8.28QTLGLGGAANDD14 pKa = 3.91STGDD18 pKa = 3.61TLRR21 pKa = 11.84VGGDD25 pKa = 3.53KK26 pKa = 11.01INDD29 pKa = 3.71NFDD32 pKa = 4.19EE33 pKa = 4.87IYY35 pKa = 9.5STLGNGSALALTTANAASGHH55 pKa = 4.23VLKK58 pKa = 11.28YY59 pKa = 9.99NGSSFVSADD68 pKa = 3.35FAVLTTSLDD77 pKa = 3.38VDD79 pKa = 3.04IHH81 pKa = 6.46GIISSSDD88 pKa = 3.05RR89 pKa = 11.84NIPITPNGTGDD100 pKa = 3.64VVITAGGTATTFEE113 pKa = 4.68GSDD116 pKa = 3.56GTVDD120 pKa = 4.39FPTSIKK126 pKa = 10.66YY127 pKa = 10.07KK128 pKa = 10.61NEE130 pKa = 3.31YY131 pKa = 8.48TAIGNAPTSATYY143 pKa = 9.62PGYY146 pKa = 10.58FFTVDD151 pKa = 3.65GDD153 pKa = 4.03DD154 pKa = 3.74NPYY157 pKa = 10.99VNMNITAGGVGDD169 pKa = 3.89TQAALLTQYY178 pKa = 11.43SSVDD182 pKa = 3.52DD183 pKa = 4.83LNDD186 pKa = 3.14VDD188 pKa = 4.22TTTNAPANDD197 pKa = 3.68QVLKK201 pKa = 10.25WSASSSKK208 pKa = 9.71WLPQDD213 pKa = 3.88DD214 pKa = 4.25QSGLTNLNLFATITADD230 pKa = 3.5TGSSTADD237 pKa = 3.12SSADD241 pKa = 3.27TLTIAGGSNITTAITGDD258 pKa = 3.47TLTIDD263 pKa = 3.91FSGVVTSTLSGMTDD277 pKa = 2.91TDD279 pKa = 4.07FTGLTQGDD287 pKa = 3.65NLFYY291 pKa = 11.03NGSSWVRR298 pKa = 11.84TASPLTWWEE307 pKa = 4.18LGSSGANDD315 pKa = 3.53YY316 pKa = 8.6TFSGPGFPVTVNDD329 pKa = 3.41PTIYY333 pKa = 9.96VYY335 pKa = 10.69RR336 pKa = 11.84GFTYY340 pKa = 10.82AFDD343 pKa = 3.77NTSNGASHH351 pKa = 7.98PFRR354 pKa = 11.84IQSTTGLSGNPYY366 pKa = 8.16TTGQSGNGQTVLYY379 pKa = 6.82WTVPMDD385 pKa = 4.19SPNTLYY391 pKa = 10.44YY392 pKa = 10.37QCTVHH397 pKa = 7.12AAMNGTIIVVNN408 pKa = 4.33

Molecular weight: 42.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3F2E4|A0A0E3F2E4_9CAUD Baseplate wedge protein OS=Synechococcus phage ACG-2014b OX=1493508 GN=Syn7803C100_90 PE=4 SV=1
MM1 pKa = 7.51AKK3 pKa = 8.91MRR5 pKa = 11.84KK6 pKa = 8.83SLSGNSMIEE15 pKa = 3.92SSPKK19 pKa = 9.03KK20 pKa = 8.74TRR22 pKa = 11.84QGTGKK27 pKa = 7.38HH28 pKa = 4.73TKK30 pKa = 9.25YY31 pKa = 10.81ASSSRR36 pKa = 11.84NAAKK40 pKa = 10.03KK41 pKa = 10.0RR42 pKa = 11.84YY43 pKa = 9.0RR44 pKa = 11.84GQGKK48 pKa = 9.52

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

215

0

215

54719

35

6422

254.5

28.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.818 ± 0.216

0.93 ± 0.099

6.694 ± 0.131

6.256 ± 0.256

4.313 ± 0.107

7.721 ± 0.325

1.413 ± 0.111

6.561 ± 0.208

5.6 ± 0.386

7.23 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.058 ± 0.221

5.903 ± 0.18

3.889 ± 0.14

3.646 ± 0.099

4.008 ± 0.155

7.37 ± 0.264

7.535 ± 0.426

6.773 ± 0.18

1.075 ± 0.082

4.209 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski