Pseudorhodobacter antarcticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudorhodobacter

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8C4W3|A0A1H8C4W3_9RHOB Myo-inositol-1(Or 4)-monophosphatase OS=Pseudorhodobacter antarcticus OX=1077947 GN=SAMN05216227_100459 PE=3 SV=1
MM1 pKa = 7.23ATNSFDD7 pKa = 4.28NPGAQTNQTINGTATADD24 pKa = 3.61TLTGGLGNDD33 pKa = 4.39TIYY36 pKa = 11.26GGAGNDD42 pKa = 3.78FLRR45 pKa = 11.84GDD47 pKa = 3.77TGVPGSWHH55 pKa = 6.22YY56 pKa = 9.49EE57 pKa = 3.76TFNFDD62 pKa = 3.14FTSANGQAFNPAAFNTTTRR81 pKa = 11.84TGSGYY86 pKa = 8.82VTDD89 pKa = 4.5FNEE92 pKa = 4.46GGITNTMRR100 pKa = 11.84GVPASTNAEE109 pKa = 4.12DD110 pKa = 3.95FGVIYY115 pKa = 10.23TSTLNTTLGGTFRR128 pKa = 11.84LTTSSDD134 pKa = 3.42DD135 pKa = 3.49GSTVQIFNSAGVALNFNNQTGGVRR159 pKa = 11.84NYY161 pKa = 10.68LNNDD165 pKa = 3.42FHH167 pKa = 8.42QGTTTRR173 pKa = 11.84WGDD176 pKa = 3.34VVLNPNEE183 pKa = 4.2TYY185 pKa = 10.37TIQIRR190 pKa = 11.84YY191 pKa = 7.17WEE193 pKa = 4.18NRR195 pKa = 11.84GADD198 pKa = 3.73TLSATISGPATGGVQNLLTSPMLGLPPPPEE228 pKa = 3.91YY229 pKa = 10.76SVTGTPMGVQGNDD242 pKa = 3.07QLFGGDD248 pKa = 4.67GNDD251 pKa = 3.3TLYY254 pKa = 11.47GDD256 pKa = 4.89GGNDD260 pKa = 3.29TLFGGNNNDD269 pKa = 3.83LLYY272 pKa = 10.94GGTGNDD278 pKa = 3.92VLNGDD283 pKa = 4.24AGADD287 pKa = 3.47TLYY290 pKa = 11.31GDD292 pKa = 5.58DD293 pKa = 6.15GNDD296 pKa = 3.45TLNGGNDD303 pKa = 3.3NDD305 pKa = 4.68LLFGGAGADD314 pKa = 3.73SLNGDD319 pKa = 3.82VGNDD323 pKa = 3.47TLYY326 pKa = 11.54GDD328 pKa = 5.02AGNDD332 pKa = 3.6TLNGGDD338 pKa = 4.29GNDD341 pKa = 4.05LLFGGADD348 pKa = 3.37NDD350 pKa = 4.09LLYY353 pKa = 11.25GGINNDD359 pKa = 3.46TLYY362 pKa = 11.48GDD364 pKa = 4.61AGADD368 pKa = 3.41ALFGGAGNDD377 pKa = 3.67LLFGGTGNDD386 pKa = 3.73TLNGGAGNNTMTGGAGDD403 pKa = 3.87DD404 pKa = 3.77TFIYY408 pKa = 10.2TPGSTLTITDD418 pKa = 4.06FNFGNSGSITDD429 pKa = 4.51SNPSNNDD436 pKa = 2.98FLDD439 pKa = 3.85LSAYY443 pKa = 8.96YY444 pKa = 10.7ASLRR448 pKa = 11.84DD449 pKa = 3.63LRR451 pKa = 11.84NDD453 pKa = 3.4FVDD456 pKa = 5.77DD457 pKa = 5.1GILNQSVGNYY467 pKa = 6.06TTTTALGGTITLTGISATDD486 pKa = 3.45LTRR489 pKa = 11.84DD490 pKa = 4.07NINVACFTLGTLIDD504 pKa = 3.93TATGPRR510 pKa = 11.84AIEE513 pKa = 3.82TLAPGTLVCTADD525 pKa = 4.45HH526 pKa = 6.67GLQPLRR532 pKa = 11.84AVLSRR537 pKa = 11.84DD538 pKa = 3.25VPGDD542 pKa = 3.44GRR544 pKa = 11.84HH545 pKa = 5.42APVMFRR551 pKa = 11.84AGALGNTRR559 pKa = 11.84DD560 pKa = 3.88MLTSPAHH567 pKa = 6.56RR568 pKa = 11.84MMITDD573 pKa = 3.7WRR575 pKa = 11.84AEE577 pKa = 3.84LLFGEE582 pKa = 5.02GEE584 pKa = 4.42VLVTARR590 pKa = 11.84ALLNDD595 pKa = 3.82HH596 pKa = 6.99SITRR600 pKa = 11.84APCARR605 pKa = 11.84VTYY608 pKa = 9.88YY609 pKa = 10.88HH610 pKa = 7.16LLLDD614 pKa = 3.59SHH616 pKa = 6.87EE617 pKa = 4.13IVYY620 pKa = 10.99AEE622 pKa = 5.01GIATEE627 pKa = 4.81SYY629 pKa = 9.09HH630 pKa = 7.06HH631 pKa = 6.48SAAVADD637 pKa = 4.11AEE639 pKa = 4.75VAAEE643 pKa = 3.93LHH645 pKa = 6.87ALFPDD650 pKa = 4.48LAQSKK655 pKa = 7.99TPSARR660 pKa = 11.84LSLRR664 pKa = 11.84GYY666 pKa = 9.51EE667 pKa = 4.07AATLLAAAA675 pKa = 5.25

Molecular weight: 70.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8FJF0|A0A1H8FJF0_9RHOB Bifunctional purine biosynthesis protein PurH OS=Pseudorhodobacter antarcticus OX=1077947 GN=purH PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84LRR21 pKa = 11.84MATKK25 pKa = 10.34AGRR28 pKa = 11.84NILNLRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3792

0

3792

1132519

24

3262

298.7

32.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.091 ± 0.068

0.903 ± 0.013

5.912 ± 0.036

4.766 ± 0.037

3.772 ± 0.023

8.831 ± 0.054

2.078 ± 0.025

5.244 ± 0.026

3.185 ± 0.035

10.067 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.972 ± 0.019

2.7 ± 0.026

5.112 ± 0.034

3.292 ± 0.022

6.44 ± 0.037

4.851 ± 0.027

5.816 ± 0.029

7.402 ± 0.041

1.408 ± 0.018

2.158 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski