Cyanobium usitatum str. Tous

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Cyanobium; Cyanobium usitatum

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2524 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P7MRB2|A0A2P7MRB2_9CYAN ABC transporter ATP-binding protein OS=Cyanobium usitatum str. Tous OX=2116684 GN=C7K55_11940 PE=4 SV=1
MM1 pKa = 7.85ADD3 pKa = 3.51ADD5 pKa = 4.15DD6 pKa = 4.66SFVAGDD12 pKa = 3.87VIAGLTEE19 pKa = 3.92NFFEE23 pKa = 4.85AVRR26 pKa = 11.84SSPDD30 pKa = 2.99LAAQEE35 pKa = 4.18ARR37 pKa = 11.84PAEE40 pKa = 4.18LVYY43 pKa = 10.84EE44 pKa = 4.44LDD46 pKa = 3.89QVVSSYY52 pKa = 11.45LEE54 pKa = 4.03EE55 pKa = 4.1QQLSSDD61 pKa = 4.32AYY63 pKa = 10.79QLIQQDD69 pKa = 4.24VLNHH73 pKa = 6.39IAHH76 pKa = 7.23DD77 pKa = 4.35LADD80 pKa = 3.73QHH82 pKa = 7.16HH83 pKa = 7.58DD84 pKa = 3.68LADD87 pKa = 4.19LDD89 pKa = 4.2VSFDD93 pKa = 3.55AAGNADD99 pKa = 4.02PAAVMAAMDD108 pKa = 3.62HH109 pKa = 6.24HH110 pKa = 6.98FEE112 pKa = 4.37ALFSSEE118 pKa = 4.37SFSDD122 pKa = 3.37DD123 pKa = 3.12GYY125 pKa = 11.66AALGG129 pKa = 3.58

Molecular weight: 13.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P7N1C3|A0A2P7N1C3_9CYAN Thioredoxin OS=Cyanobium usitatum str. Tous OX=2116684 GN=C7K55_00345 PE=3 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.03RR4 pKa = 11.84TLEE7 pKa = 3.92GTSRR11 pKa = 11.84KK12 pKa = 9.24RR13 pKa = 11.84KK14 pKa = 7.95RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.21TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.5

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2524

0

2524

738024

30

4888

292.4

31.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.928 ± 0.066

1.166 ± 0.019

4.755 ± 0.054

5.725 ± 0.051

3.01 ± 0.031

8.797 ± 0.065

1.957 ± 0.03

3.962 ± 0.037

2.393 ± 0.04

13.199 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.788 ± 0.023

2.454 ± 0.042

5.874 ± 0.042

4.915 ± 0.041

7.04 ± 0.05

6.086 ± 0.042

4.46 ± 0.047

6.932 ± 0.046

1.721 ± 0.028

1.837 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski