Lysobacter defluvii IMMIB APB-9 = DSM 18482

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; Lysobacter defluvii

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2443 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0M3L4|A0A0A0M3L4_9GAMM Nitrogen regulation protein B OS=Lysobacter defluvii IMMIB APB-9 = DSM 18482 OX=1385515 GN=N791_08235 PE=4 SV=1
MM1 pKa = 7.41SKK3 pKa = 10.67DD4 pKa = 3.43ATPLRR9 pKa = 11.84SWMCVVCGFIYY20 pKa = 10.65DD21 pKa = 3.84EE22 pKa = 4.38AAGLPEE28 pKa = 4.46EE29 pKa = 5.49GIAPGTRR36 pKa = 11.84WEE38 pKa = 4.7DD39 pKa = 3.78VPDD42 pKa = 3.14TWTCPDD48 pKa = 4.11CGTTKK53 pKa = 11.08DD54 pKa = 3.91DD55 pKa = 4.23FEE57 pKa = 4.51MVALL61 pKa = 4.43

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0M392|A0A0A0M392_9GAMM FAD_binding_3 domain-containing protein OS=Lysobacter defluvii IMMIB APB-9 = DSM 18482 OX=1385515 GN=N791_09345 PE=4 SV=1
MM1 pKa = 6.64FTSPKK6 pKa = 9.39RR7 pKa = 11.84YY8 pKa = 8.16GVSSHH13 pKa = 5.52RR14 pKa = 11.84HH15 pKa = 4.41KK16 pKa = 11.04ALNARR21 pKa = 11.84LITAAMPMASKK32 pKa = 9.95MGTSGWARR40 pKa = 11.84RR41 pKa = 11.84QPIWMATATAAPRR54 pKa = 11.84ISKK57 pKa = 9.63PLTSATLALARR68 pKa = 11.84RR69 pKa = 11.84LAPITARR76 pKa = 11.84LSRR79 pKa = 3.9

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2443

0

2443

739019

41

1989

302.5

32.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.797 ± 0.062

0.758 ± 0.017

5.994 ± 0.042

6.276 ± 0.052

3.241 ± 0.036

9.278 ± 0.054

2.246 ± 0.028

3.8 ± 0.036

2.158 ± 0.04

10.802 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.275 ± 0.023

2.231 ± 0.031

5.539 ± 0.032

3.356 ± 0.032

8.161 ± 0.051

4.829 ± 0.034

4.677 ± 0.032

7.89 ± 0.045

1.526 ± 0.025

2.168 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski