Synechococcus phage ACG-2014d

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3HXY0|A0A0E3HXY0_9CAUD Uncharacterized protein OS=Synechococcus phage ACG-2014d OX=1493509 GN=Syn7803C102_172 PE=4 SV=1
MM1 pKa = 7.17ATNTGALANIKK12 pKa = 9.65PSSGTFTTLFKK23 pKa = 11.22NDD25 pKa = 3.64VLSSTTGTVFVNCDD39 pKa = 2.66GTGADD44 pKa = 3.89TYY46 pKa = 11.17NIKK49 pKa = 10.6LNRR52 pKa = 11.84WDD54 pKa = 4.1QEE56 pKa = 4.02LTLDD60 pKa = 3.57ANTYY64 pKa = 9.72LLHH67 pKa = 7.15RR68 pKa = 11.84GDD70 pKa = 5.33LISNVKK76 pKa = 7.51WTLSASIPLADD87 pKa = 6.09AIPGTKK93 pKa = 9.46FVSSDD98 pKa = 3.67GEE100 pKa = 4.02KK101 pKa = 10.29CAYY104 pKa = 9.95LLDD107 pKa = 3.96VADD110 pKa = 4.78PVTTTYY116 pKa = 10.21EE117 pKa = 3.71VRR119 pKa = 11.84YY120 pKa = 9.43KK121 pKa = 11.01SLIAFTLEE129 pKa = 3.81NVADD133 pKa = 4.03TGSSVNPDD141 pKa = 3.38YY142 pKa = 11.88ANGEE146 pKa = 4.45TVSNGGGVSGVVYY159 pKa = 10.38EE160 pKa = 5.1NIPGSNDD167 pKa = 4.12DD168 pKa = 3.56AVLWIGDD175 pKa = 3.45ITGGTFSEE183 pKa = 4.66GDD185 pKa = 3.75VLTGGSSTTSGTVSTGGIATAANRR209 pKa = 11.84FVFNDD214 pKa = 4.0GAGGAVFRR222 pKa = 11.84LQNEE226 pKa = 4.16IQPEE230 pKa = 4.26LLTDD234 pKa = 3.84RR235 pKa = 11.84VYY237 pKa = 11.3KK238 pKa = 10.56FDD240 pKa = 3.52VANASMSGKK249 pKa = 9.52ILEE252 pKa = 4.63FSDD255 pKa = 4.04TNGGSNNSGDD265 pKa = 3.66EE266 pKa = 4.08FVTGKK271 pKa = 8.13TVSGTPGQAGAFVQYY286 pKa = 10.86NLTGAEE292 pKa = 4.7LISNFYY298 pKa = 10.55PYY300 pKa = 10.79DD301 pKa = 3.62QADD304 pKa = 3.22ATYY307 pKa = 10.9ADD309 pKa = 4.08DD310 pKa = 3.61SQYY313 pKa = 9.75FTFSEE318 pKa = 4.9EE319 pKa = 4.04YY320 pKa = 8.93TFNEE324 pKa = 4.19IYY326 pKa = 10.77VYY328 pKa = 7.45TQKK331 pKa = 11.12DD332 pKa = 3.95EE333 pKa = 4.22YY334 pKa = 11.06LQTPDD339 pKa = 2.56QWIITDD345 pKa = 3.54AFIYY349 pKa = 10.43RR350 pKa = 11.84DD351 pKa = 3.21VTYY354 pKa = 10.67AVDD357 pKa = 5.39AIAGDD362 pKa = 3.72SYY364 pKa = 11.3GTILEE369 pKa = 4.11WDD371 pKa = 3.59KK372 pKa = 11.7VNSKK376 pKa = 10.82LYY378 pKa = 9.23VANGPGSAAWAGSDD392 pKa = 3.49TFFEE396 pKa = 4.73SPRR399 pKa = 11.84QLSVSKK405 pKa = 9.88ATATINSVLSSPADD419 pKa = 3.68DD420 pKa = 4.62FIVRR424 pKa = 11.84ADD426 pKa = 4.84AIAQEE431 pKa = 4.32TTEE434 pKa = 4.23RR435 pKa = 11.84QTGIIIGPGQSIQVEE450 pKa = 4.64CTNGRR455 pKa = 11.84CNFVFDD461 pKa = 5.03AFQDD465 pKa = 3.86TVNEE469 pKa = 4.35VVTALYY475 pKa = 10.07QRR477 pKa = 11.84SSDD480 pKa = 3.82YY481 pKa = 9.65QTGLEE486 pKa = 4.1DD487 pKa = 4.62SGDD490 pKa = 3.85GGDD493 pKa = 5.04GGDD496 pKa = 3.35

Molecular weight: 52.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3FUR1|A0A0E3FUR1_9CAUD Uncharacterized protein OS=Synechococcus phage ACG-2014d OX=1493509 GN=Syn7803C102_45 PE=4 SV=1
MM1 pKa = 7.56LSLVSIIGSSPVEE14 pKa = 3.67AKK16 pKa = 10.57GNKK19 pKa = 9.23LSGRR23 pKa = 11.84PGVSVRR29 pKa = 11.84PRR31 pKa = 11.84KK32 pKa = 10.13CNLCQPKK39 pKa = 9.69VRR41 pKa = 11.84IPKK44 pKa = 9.72KK45 pKa = 10.7CNLCQGGHH53 pKa = 6.2RR54 pKa = 11.84FGKK57 pKa = 9.01TKK59 pKa = 8.3MTFF62 pKa = 2.92

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

218

0

218

57254

35

6817

262.6

29.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.803 ± 0.204

0.973 ± 0.091

6.798 ± 0.133

6.141 ± 0.229

4.281 ± 0.107

7.813 ± 0.287

1.423 ± 0.109

6.351 ± 0.215

5.78 ± 0.447

7.166 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.077 ± 0.212

5.879 ± 0.157

4.084 ± 0.145

3.745 ± 0.086

3.877 ± 0.158

7.421 ± 0.217

7.282 ± 0.39

6.642 ± 0.183

1.165 ± 0.095

4.297 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski