Achromatium sp. WMS2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Thiotrichaceae; Achromatium; unclassified Achromatium

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2198 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M1JDG9|A0A0M1JDG9_9GAMM Sulfurtransferase OS=Achromatium sp. WMS2 OX=1604835 GN=TI04_04180 PE=3 SV=1
MM1 pKa = 7.22LQHH4 pKa = 7.37PDD6 pKa = 3.82FNPVDD11 pKa = 3.57INADD15 pKa = 3.51VNDD18 pKa = 4.4CYY20 pKa = 10.87IVIRR24 pKa = 11.84IDD26 pKa = 3.29TLLFLVPQTQVYY38 pKa = 10.07ALEE41 pKa = 4.19PAFDD45 pKa = 3.58VEE47 pKa = 4.31YY48 pKa = 11.11LKK50 pKa = 10.57DD51 pKa = 3.26ICNLEE56 pKa = 4.53DD57 pKa = 5.16EE58 pKa = 4.98IEE60 pKa = 4.74CDD62 pKa = 3.89DD63 pKa = 4.33SLAINVNSSKK73 pKa = 11.14ALGQLQIDD81 pKa = 4.05DD82 pKa = 3.91QDD84 pKa = 3.98YY85 pKa = 9.81PIYY88 pKa = 10.69GLSADD93 pKa = 4.92LKK95 pKa = 9.61PISKK99 pKa = 10.01VPEE102 pKa = 3.75TRR104 pKa = 11.84RR105 pKa = 11.84ICVLFNTPSQVFGLLADD122 pKa = 3.59QVVLLEE128 pKa = 4.21FQEE131 pKa = 4.12EE132 pKa = 4.31LNIRR136 pKa = 11.84KK137 pKa = 9.01LPICMQTGNSRR148 pKa = 11.84IRR150 pKa = 11.84GLVLKK155 pKa = 10.15DD156 pKa = 3.34QDD158 pKa = 4.03VLCVIEE164 pKa = 5.79AIDD167 pKa = 3.57ILAYY171 pKa = 8.54CTNPVIEE178 pKa = 4.27EE179 pKa = 4.14QYY181 pKa = 11.31GG182 pKa = 3.37

Molecular weight: 20.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M1J7U1|A0A0M1J7U1_9GAMM 50S ribosomal protein L33 OS=Achromatium sp. WMS2 OX=1604835 GN=rpmG PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84KK29 pKa = 9.19VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.21GRR39 pKa = 11.84ARR41 pKa = 11.84LIPP44 pKa = 4.0

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2198

0

2198

583320

44

1868

265.4

29.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.939 ± 0.064

1.104 ± 0.022

5.031 ± 0.037

5.085 ± 0.053

3.617 ± 0.037

6.924 ± 0.057

2.313 ± 0.026

7.163 ± 0.048

4.421 ± 0.042

10.89 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.023

4.587 ± 0.043

4.699 ± 0.037

4.673 ± 0.046

5.233 ± 0.047

5.996 ± 0.042

6.02 ± 0.062

6.552 ± 0.043

1.372 ± 0.023

3.087 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski