Melon necrotic spot virus (MNSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Gammacarmovirus

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q89846|MP2_MNSV Double gene block protein 2 OS=Melon necrotic spot virus OX=11987 GN=ORF3 PE=1 SV=1
MM1 pKa = 7.57ACCRR5 pKa = 11.84CDD7 pKa = 3.7SSPGDD12 pKa = 3.38YY13 pKa = 10.85SGALLILFISFVFFYY28 pKa = 9.43ITSLSPQGNTYY39 pKa = 8.65VHH41 pKa = 6.79HH42 pKa = 7.17FDD44 pKa = 3.44SSSVKK49 pKa = 7.69TQYY52 pKa = 11.22VGISTNGDD60 pKa = 3.11GG61 pKa = 4.98

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q89846|MP2_MNSV Double gene block protein 2 OS=Melon necrotic spot virus OX=11987 GN=ORF3 PE=1 SV=1
MM1 pKa = 8.11DD2 pKa = 3.57SQRR5 pKa = 11.84TVEE8 pKa = 4.04LTNPRR13 pKa = 11.84GRR15 pKa = 11.84SKK17 pKa = 10.99EE18 pKa = 3.97RR19 pKa = 11.84GDD21 pKa = 4.34SGGKK25 pKa = 8.97QKK27 pKa = 11.39NSMGRR32 pKa = 11.84KK33 pKa = 7.57IANDD37 pKa = 4.35AISEE41 pKa = 4.31SKK43 pKa = 10.1QGVMGASTYY52 pKa = 10.45IADD55 pKa = 4.54KK56 pKa = 10.83IKK58 pKa = 10.11VTINFNFF65 pKa = 3.54

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1703

61

791

283.8

31.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.87 ± 0.708

2.231 ± 0.451

5.461 ± 0.23

4.052 ± 0.818

3.699 ± 0.56

7.927 ± 0.514

2.173 ± 0.466

6.87 ± 0.539

5.461 ± 0.608

7.692 ± 0.897

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.173 ± 0.267

4.991 ± 0.472

3.934 ± 0.467

2.466 ± 0.359

6.107 ± 0.649

9.571 ± 1.147

5.637 ± 0.754

8.103 ± 0.693

1.409 ± 0.364

3.053 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski