Planctomycetes bacterium SV_7m_r

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517SXU4|A0A517SXU4_9BACT Phage-related baseplate assembly protein OS=Planctomycetes bacterium SV_7m_r OX=2528025 GN=SV7mr_34020 PE=3 SV=1
MM1 pKa = 7.2SVLYY5 pKa = 9.93IALPIAILLGAGGLIACLYY24 pKa = 9.35CIKK27 pKa = 10.64DD28 pKa = 3.55GQYY31 pKa = 11.46DD32 pKa = 3.94DD33 pKa = 5.7LDD35 pKa = 4.29SPSVRR40 pKa = 11.84MLIDD44 pKa = 4.25DD45 pKa = 4.67EE46 pKa = 4.67PVQQAAEE53 pKa = 4.11SDD55 pKa = 3.73ADD57 pKa = 3.4AHH59 pKa = 5.35EE60 pKa = 4.72AKK62 pKa = 10.61LL63 pKa = 3.66

Molecular weight: 6.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517SNK8|A0A517SNK8_9BACT Methionine--tRNA ligase OS=Planctomycetes bacterium SV_7m_r OX=2528025 GN=metG PE=3 SV=1
MM1 pKa = 7.91AKK3 pKa = 9.65PQHH6 pKa = 6.16KK7 pKa = 9.83LKK9 pKa = 10.41KK10 pKa = 8.54ANHH13 pKa = 5.37GRR15 pKa = 11.84RR16 pKa = 11.84PASAKK21 pKa = 9.66ARR23 pKa = 11.84KK24 pKa = 9.25AKK26 pKa = 9.98RR27 pKa = 11.84KK28 pKa = 9.47KK29 pKa = 10.27IKK31 pKa = 9.22TT32 pKa = 3.56

Molecular weight: 3.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5245

0

5245

2028258

29

12437

386.7

42.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.671 ± 0.032

1.161 ± 0.017

6.283 ± 0.042

5.678 ± 0.034

3.574 ± 0.02

7.506 ± 0.048

2.25 ± 0.019

4.941 ± 0.022

3.803 ± 0.038

9.74 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.021

3.368 ± 0.028

5.313 ± 0.03

5.139 ± 0.038

6.286 ± 0.038

6.764 ± 0.031

5.543 ± 0.038

6.939 ± 0.03

1.446 ± 0.017

2.328 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski