Microbacterium phage Ariadne

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Metamorphoovirus; unclassified Metamorphoovirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VAY7|A0A649VAY7_9CAUD Uncharacterized protein OS=Microbacterium phage Ariadne OX=2656546 GN=83 PE=4 SV=1
MM1 pKa = 7.93IMALHH6 pKa = 7.23DD7 pKa = 4.42SPVSQSDD14 pKa = 3.31VDD16 pKa = 4.84DD17 pKa = 5.09VINDD21 pKa = 3.2AHH23 pKa = 5.63EE24 pKa = 4.42QIDD27 pKa = 3.83NGGSRR32 pKa = 11.84WPGMSYY38 pKa = 10.45EE39 pKa = 4.1QGLVAGLQWVTGEE52 pKa = 4.45TDD54 pKa = 3.13DD55 pKa = 5.61HH56 pKa = 6.72PYY58 pKa = 10.82EE59 pKa = 5.96DD60 pKa = 4.09EE61 pKa = 4.08

Molecular weight: 6.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VB44|A0A649VB44_9CAUD Uncharacterized protein OS=Microbacterium phage Ariadne OX=2656546 GN=19 PE=4 SV=1
MM1 pKa = 7.15TLIAARR7 pKa = 11.84VRR9 pKa = 11.84RR10 pKa = 11.84APHH13 pKa = 6.99PEE15 pKa = 3.94TLTVPAKK22 pKa = 10.22FVVHH26 pKa = 6.84RR27 pKa = 11.84DD28 pKa = 3.49VNGSGRR34 pKa = 11.84WEE36 pKa = 3.85VHH38 pKa = 6.38AKK40 pKa = 10.26DD41 pKa = 3.72SPYY44 pKa = 11.08CEE46 pKa = 4.16VTHH49 pKa = 6.55PGWSGNEE56 pKa = 3.74PGGCLGLSVPRR67 pKa = 11.84GDD69 pKa = 3.19WARR72 pKa = 11.84AVAEE76 pKa = 3.8AHH78 pKa = 6.16ARR80 pKa = 11.84SSAARR85 pKa = 11.84LAFRR89 pKa = 11.84DD90 pKa = 3.5RR91 pKa = 11.84LQARR95 pKa = 11.84GRR97 pKa = 11.84FFSS100 pKa = 3.58

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

17303

37

956

173.0

18.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.9 ± 0.508

0.971 ± 0.141

6.571 ± 0.398

6.49 ± 0.379

2.427 ± 0.123

8.773 ± 0.367

1.953 ± 0.181

4.485 ± 0.246

2.422 ± 0.177

7.6 ± 0.364

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.144

2.682 ± 0.178

6.294 ± 0.296

3.335 ± 0.147

7.033 ± 0.412

5.652 ± 0.247

7.039 ± 0.295

7.369 ± 0.224

2.075 ± 0.185

2.433 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski