Bifidobacterium stellenboschense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087DMY7|A0A087DMY7_9BIFI Uncharacterized protein OS=Bifidobacterium stellenboschense OX=762211 GN=BSTEL_1796 PE=4 SV=1
MM1 pKa = 7.25QSNPTSIVPSHH12 pKa = 6.69IAPYY16 pKa = 9.68EE17 pKa = 3.62IRR19 pKa = 11.84VYY21 pKa = 10.3VANLAAYY28 pKa = 9.08NAGFMRR34 pKa = 11.84GDD36 pKa = 4.36WITLPVSQDD45 pKa = 3.33EE46 pKa = 4.61LDD48 pKa = 3.81GFLDD52 pKa = 3.36EE53 pKa = 6.79RR54 pKa = 11.84VFQWHH59 pKa = 6.37GADD62 pKa = 4.34DD63 pKa = 4.36EE64 pKa = 4.66YY65 pKa = 11.42AIHH68 pKa = 7.4DD69 pKa = 3.96FEE71 pKa = 7.21KK72 pKa = 11.02DD73 pKa = 3.39GLLKK77 pKa = 10.75RR78 pKa = 11.84LDD80 pKa = 3.45WTVYY84 pKa = 9.87EE85 pKa = 4.18YY86 pKa = 11.29QDD88 pKa = 4.44LDD90 pKa = 3.96EE91 pKa = 5.6LNALATLAKK100 pKa = 9.86EE101 pKa = 4.27HH102 pKa = 6.81LATDD106 pKa = 3.92EE107 pKa = 4.1DD108 pKa = 4.03WDD110 pKa = 4.18RR111 pKa = 11.84LEE113 pKa = 5.44LAIDD117 pKa = 3.61YY118 pKa = 7.33CLPGASDD125 pKa = 5.83ALMLCNAILQLDD137 pKa = 3.81DD138 pKa = 3.83MAYY141 pKa = 10.45SPYY144 pKa = 10.8DD145 pKa = 3.36LPNNVTIEE153 pKa = 4.07NTPDD157 pKa = 2.98RR158 pKa = 11.84EE159 pKa = 3.87EE160 pKa = 4.35RR161 pKa = 11.84YY162 pKa = 10.56GYY164 pKa = 10.87DD165 pKa = 4.18KK166 pKa = 10.92INRR169 pKa = 11.84DD170 pKa = 3.13PDD172 pKa = 3.34VRR174 pKa = 11.84HH175 pKa = 6.69AILDD179 pKa = 3.83GSLDD183 pKa = 3.35IYY185 pKa = 11.17FDD187 pKa = 3.45FAKK190 pKa = 10.64YY191 pKa = 10.61GRR193 pKa = 11.84DD194 pKa = 3.59DD195 pKa = 3.52VASGYY200 pKa = 10.61VEE202 pKa = 4.32LGDD205 pKa = 4.47YY206 pKa = 10.81GWLDD210 pKa = 3.43TSQPVPDD217 pKa = 3.78ADD219 pKa = 3.42KK220 pKa = 9.86YY221 pKa = 11.53DD222 pKa = 3.72RR223 pKa = 11.84YY224 pKa = 10.56EE225 pKa = 4.02VLDD228 pKa = 3.9AAGLGG233 pKa = 3.73

Molecular weight: 26.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087D7Y0|A0A087D7Y0_9BIFI Diguanylate cyclase OS=Bifidobacterium stellenboschense OX=762211 GN=BSTEL_2213 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.62HH16 pKa = 6.11GFRR19 pKa = 11.84QRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 6.57TLAAA44 pKa = 4.49

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2202

0

2202

805556

34

3149

365.8

39.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.849 ± 0.074

0.875 ± 0.015

6.924 ± 0.052

5.469 ± 0.057

3.368 ± 0.033

8.478 ± 0.051

2.096 ± 0.026

4.939 ± 0.04

3.773 ± 0.049

8.589 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.029

3.006 ± 0.038

4.817 ± 0.039

2.946 ± 0.029

6.407 ± 0.07

5.592 ± 0.038

6.404 ± 0.069

7.87 ± 0.047

1.33 ± 0.021

2.713 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski