Richelia intracellularis HM01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Richelia; Richelia intracellularis

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1674 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1WVD8|M1WVD8_9NOST Heterocyst-specific glycolipids-directing protein OS=Richelia intracellularis HM01 OX=1165095 GN=RINTHM_5760 PE=4 SV=1
MM1 pKa = 7.8DD2 pKa = 4.6TLGMLIGLDD11 pKa = 3.42VQSQYY16 pKa = 11.64EE17 pKa = 4.12DD18 pKa = 3.44AQAHH22 pKa = 6.46LEE24 pKa = 4.27PGDD27 pKa = 3.72TIIYY31 pKa = 7.75YY32 pKa = 10.03TDD34 pKa = 3.45GLTDD38 pKa = 3.78AAAADD43 pKa = 3.89GNRR46 pKa = 11.84FDD48 pKa = 4.16EE49 pKa = 4.74EE50 pKa = 4.11NLISHH55 pKa = 7.28LSFACRR61 pKa = 11.84YY62 pKa = 9.19CSSTQEE68 pKa = 3.78ILDD71 pKa = 3.72YY72 pKa = 11.44LFDD75 pKa = 3.8QVQKK79 pKa = 10.96FIGANKK85 pKa = 9.73QSNDD89 pKa = 3.17DD90 pKa = 3.49MTLVVLQVRR99 pKa = 4.4

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1WV27|M1WV27_9NOST Uncharacterized protein OS=Richelia intracellularis HM01 OX=1165095 GN=RINTHM_2460 PE=4 SV=1
MM1 pKa = 7.17SRR3 pKa = 11.84YY4 pKa = 9.52RR5 pKa = 11.84GPRR8 pKa = 11.84LRR10 pKa = 11.84ITRR13 pKa = 11.84RR14 pKa = 11.84LGEE17 pKa = 4.22LPGLTRR23 pKa = 11.84KK24 pKa = 9.04SARR27 pKa = 11.84RR28 pKa = 11.84AYY30 pKa = 9.88PPGQHH35 pKa = 5.24GQNRR39 pKa = 11.84KK40 pKa = 9.27KK41 pKa = 10.18RR42 pKa = 11.84SEE44 pKa = 3.92YY45 pKa = 10.72AVRR48 pKa = 11.84LEE50 pKa = 4.23EE51 pKa = 4.12KK52 pKa = 10.23QKK54 pKa = 11.1LRR56 pKa = 11.84FNYY59 pKa = 9.88GLSEE63 pKa = 4.22SQLLRR68 pKa = 11.84YY69 pKa = 8.64IRR71 pKa = 11.84KK72 pKa = 8.69ARR74 pKa = 11.84RR75 pKa = 11.84VTGSTGQVLLQLLEE89 pKa = 4.0MRR91 pKa = 11.84LDD93 pKa = 3.36NTTFRR98 pKa = 11.84LGLAPTIPAARR109 pKa = 11.84QLVNHH114 pKa = 5.97GHH116 pKa = 5.63ITVNSKK122 pKa = 9.49VVNIASYY129 pKa = 8.06QCRR132 pKa = 11.84PGEE135 pKa = 4.25VISIINRR142 pKa = 11.84DD143 pKa = 3.35KK144 pKa = 10.79SRR146 pKa = 11.84QMVEE150 pKa = 3.96TNLQYY155 pKa = 10.64PGLANLPSHH164 pKa = 7.36LEE166 pKa = 3.61FDD168 pKa = 3.84KK169 pKa = 11.6NKK171 pKa = 10.83LEE173 pKa = 4.16GKK175 pKa = 9.11INSVIEE181 pKa = 4.25RR182 pKa = 11.84EE183 pKa = 4.3WVALQINEE191 pKa = 4.28LLVVEE196 pKa = 5.04YY197 pKa = 9.28YY198 pKa = 11.26SRR200 pKa = 11.84MAA202 pKa = 4.45

Molecular weight: 23.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1674

0

1674

329974

37

1318

197.1

22.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.018 ± 0.07

1.198 ± 0.024

4.498 ± 0.051

5.968 ± 0.066

3.866 ± 0.055

6.659 ± 0.067

2.043 ± 0.033

8.174 ± 0.062

5.619 ± 0.056

10.932 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.03

4.862 ± 0.056

4.249 ± 0.036

4.831 ± 0.059

4.779 ± 0.05

6.578 ± 0.054

5.353 ± 0.042

6.393 ± 0.044

1.344 ± 0.03

3.333 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski