Streptomyces globosus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6315 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A344U3P2|A0A344U3P2_9ACTN SCO1431 family membrane protein OS=Streptomyces globosus OX=68209 GN=C0216_20545 PE=4 SV=1
MM1 pKa = 7.89SDD3 pKa = 2.8TLYY6 pKa = 10.47PGGEE10 pKa = 3.82IGLGQSLQGGAYY22 pKa = 8.92TLTLQYY28 pKa = 11.01DD29 pKa = 3.95GNLVLSEE36 pKa = 4.17PSGSVVWATDD46 pKa = 3.05THH48 pKa = 5.39EE49 pKa = 4.33QGVQRR54 pKa = 11.84AVLQDD59 pKa = 3.57DD60 pKa = 4.64GNFVLYY66 pKa = 10.58SDD68 pKa = 5.08YY69 pKa = 11.37GPVWATDD76 pKa = 3.19THH78 pKa = 7.09GNDD81 pKa = 3.05AGRR84 pKa = 11.84LVVQADD90 pKa = 4.19RR91 pKa = 11.84NVVLYY96 pKa = 10.49AVDD99 pKa = 5.57GSPLWEE105 pKa = 4.73SDD107 pKa = 3.41TNTDD111 pKa = 3.45NPIHH115 pKa = 7.3LEE117 pKa = 4.11EE118 pKa = 4.54PAAAPVAEE126 pKa = 4.6WEE128 pKa = 4.4APAIPEE134 pKa = 3.87PRR136 pKa = 11.84TYY138 pKa = 10.07TVEE141 pKa = 4.45PGDD144 pKa = 4.12TLWDD148 pKa = 3.26IAEE151 pKa = 4.22RR152 pKa = 11.84FYY154 pKa = 11.66GDD156 pKa = 3.12GNRR159 pKa = 11.84YY160 pKa = 9.23HH161 pKa = 7.77DD162 pKa = 3.62IAAASGIADD171 pKa = 4.31PDD173 pKa = 3.88LVDD176 pKa = 3.34VGQVLTIPP184 pKa = 4.06

Molecular weight: 19.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A344U8H4|A0A344U8H4_9ACTN Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Streptomyces globosus OX=68209 GN=C0216_00350 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84SALSAA45 pKa = 3.8

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6315

0

6315

2132909

24

10285

337.8

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.188 ± 0.064

0.809 ± 0.008

5.729 ± 0.028

5.631 ± 0.029

2.652 ± 0.018

9.816 ± 0.032

2.256 ± 0.013

2.809 ± 0.023

1.941 ± 0.027

10.346 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.662 ± 0.013

1.59 ± 0.019

6.541 ± 0.031

2.615 ± 0.019

8.365 ± 0.035

4.712 ± 0.022

5.659 ± 0.026

8.212 ± 0.03

1.496 ± 0.012

1.971 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski