Enterobacteria phage phiP27

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8W615|Q8W615_9CAUD Baseplate_J domain-containing protein OS=Enterobacteria phage phiP27 OX=103807 PE=4 SV=1
MM1 pKa = 7.89DD2 pKa = 5.19TLQDD6 pKa = 4.3DD7 pKa = 4.47YY8 pKa = 11.98TKK10 pKa = 11.06LLYY13 pKa = 11.03GLMPPGPAWSDD24 pKa = 2.95TDD26 pKa = 3.84GVLDD30 pKa = 3.97GLAPSLVRR38 pKa = 11.84VHH40 pKa = 5.99QRR42 pKa = 11.84ADD44 pKa = 3.42EE45 pKa = 4.07LVIEE49 pKa = 4.76IDD51 pKa = 3.54PGQSTEE57 pKa = 4.07LIEE60 pKa = 5.22RR61 pKa = 11.84YY62 pKa = 9.69EE63 pKa = 4.09EE64 pKa = 5.04LYY66 pKa = 10.92GLPDD70 pKa = 3.16SCSPVGTRR78 pKa = 11.84TLRR81 pKa = 11.84QRR83 pKa = 11.84QQRR86 pKa = 11.84LEE88 pKa = 3.99AKK90 pKa = 10.03ANVAGGINEE99 pKa = 4.25QFFLDD104 pKa = 3.5QLEE107 pKa = 4.27ALGYY111 pKa = 8.47TGVTIEE117 pKa = 4.61QFQNLDD123 pKa = 3.43ASPDD127 pKa = 3.95PEE129 pKa = 4.16WGDD132 pKa = 2.73RR133 pKa = 11.84WRR135 pKa = 11.84YY136 pKa = 6.94FWRR139 pKa = 11.84VTLPVDD145 pKa = 5.65AGAQWQTCTDD155 pKa = 3.29ACNTPIRR162 pKa = 11.84TWGDD166 pKa = 3.4TVAEE170 pKa = 4.09CVINKK175 pKa = 8.46LCPSHH180 pKa = 6.22TVVLFSYY187 pKa = 10.44PDD189 pKa = 3.6EE190 pKa = 6.71DD191 pKa = 5.24EE192 pKa = 4.88DD193 pKa = 5.09AQDD196 pKa = 3.19

Molecular weight: 22.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9MBZ6|Q9MBZ6_9CAUD Uncharacterized protein OS=Enterobacteria phage phiP27 OX=103807 PE=4 SV=1
MM1 pKa = 7.66LSNLPGLLNVALCAVIVLTLFFYY24 pKa = 10.7RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84DD28 pKa = 3.54SRR30 pKa = 11.84HH31 pKa = 5.61KK32 pKa = 10.64PLMSWLAWLLMLLYY46 pKa = 10.77AFAPLSYY53 pKa = 10.95LCGRR57 pKa = 11.84PLATGWLEE65 pKa = 4.02VFFNLLFCVLVIRR78 pKa = 11.84ARR80 pKa = 11.84GNVTKK85 pKa = 10.56IFPLLRR91 pKa = 5.39

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12749

51

649

219.8

24.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.02 ± 0.272

1.184 ± 0.14

5.875 ± 0.23

6.808 ± 0.321

3.514 ± 0.151

7.467 ± 0.31

1.914 ± 0.138

5.475 ± 0.229

5.459 ± 0.382

8.895 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.152

3.992 ± 0.188

4.087 ± 0.181

4.259 ± 0.194

6.157 ± 0.259

6.016 ± 0.266

5.875 ± 0.264

6.518 ± 0.223

1.545 ± 0.11

3.13 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski