Salmonella phage SKML-39

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Aglimvirinae; Agtrevirus; Salmonella virus SKML39

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4I3L6|K4I3L6_9CAUD Uncharacterized protein OS=Salmonella phage SKML-39 OX=1204528 PE=4 SV=1
MM1 pKa = 7.76NITPSQTGYY10 pKa = 9.37EE11 pKa = 4.28HH12 pKa = 6.87ILVFIALQGAKK23 pKa = 9.93EE24 pKa = 4.17GIIVSEE30 pKa = 3.94YY31 pKa = 10.59SGTVRR36 pKa = 11.84LADD39 pKa = 3.59YY40 pKa = 8.54VAPEE44 pKa = 4.03AFIKK48 pKa = 10.47KK49 pKa = 10.35CIDD52 pKa = 3.08AWCLYY57 pKa = 11.3ALGTYY62 pKa = 7.83PAPPEE67 pKa = 4.38GEE69 pKa = 4.3LLFTVPTLAAGDD81 pKa = 3.78TDD83 pKa = 6.15AIFADD88 pKa = 4.4MLAKK92 pKa = 10.47GYY94 pKa = 10.18LVYY97 pKa = 10.75DD98 pKa = 3.78ITHH101 pKa = 7.01DD102 pKa = 3.78VYY104 pKa = 11.7SFTSLNTVFGFTITSEE120 pKa = 4.18ANIPDD125 pKa = 3.76WGILAWGVDD134 pKa = 4.04GLGATYY140 pKa = 10.69ASSDD144 pKa = 3.54VGAVLIDD151 pKa = 3.56EE152 pKa = 4.84GVTMEE157 pKa = 4.44QYY159 pKa = 10.62LAQFPQHH166 pKa = 6.73NITGSDD172 pKa = 3.62IILVQPGAIALPRR185 pKa = 11.84LVDD188 pKa = 3.42GWKK191 pKa = 9.34PQLKK195 pKa = 10.37DD196 pKa = 3.31IIAGIRR202 pKa = 11.84FHH204 pKa = 8.16IPVVNPTPP212 pKa = 3.59

Molecular weight: 22.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4I2Z2|K4I2Z2_9CAUD Sliding clamp DNA polymerase accessory protein OS=Salmonella phage SKML-39 OX=1204528 PE=4 SV=1
MM1 pKa = 7.53ANSIPILEE9 pKa = 4.45AVVKK13 pKa = 9.32EE14 pKa = 4.02NPRR17 pKa = 11.84ARR19 pKa = 11.84KK20 pKa = 8.75SRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84LVTKK28 pKa = 10.72LDD30 pKa = 3.64LLKK33 pKa = 10.83QKK35 pKa = 10.83FGGHH39 pKa = 5.04SVFTRR44 pKa = 11.84IRR46 pKa = 11.84NALEE50 pKa = 3.7EE51 pKa = 4.29GRR53 pKa = 11.84TEE55 pKa = 4.36LEE57 pKa = 3.95LYY59 pKa = 10.03RR60 pKa = 11.84PNGSTRR66 pKa = 11.84AYY68 pKa = 8.21QTTDD72 pKa = 2.69GLLEE76 pKa = 4.85LIRR79 pKa = 11.84LSGMTIEE86 pKa = 4.9PRR88 pKa = 11.84SSGTPLCSLYY98 pKa = 11.17VIGNLGALL106 pKa = 3.84

Molecular weight: 11.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

208

0

208

48990

38

1612

235.5

26.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.22 ± 0.192

1.133 ± 0.082

6.542 ± 0.121

6.534 ± 0.2

4.278 ± 0.127

6.91 ± 0.228

1.89 ± 0.1

6.036 ± 0.121

6.397 ± 0.178

8.116 ± 0.138

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.821 ± 0.104

4.921 ± 0.146

3.895 ± 0.113

3.778 ± 0.112

4.923 ± 0.139

6.007 ± 0.196

5.983 ± 0.22

7.467 ± 0.179

1.39 ± 0.088

3.758 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski