Proteome-pI 2.0 - Proteome Isoelectric Point Database

Mycoplasma wenyonii (strain Massachusetts) (Eperythrozoon wenyonii)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma wenyonii

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 651 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|I6YMB9|I6YMB9_MYCWM 16S ribosomal RNA methyltransferase KsgA/Dim1 family protein OS=Mycoplasma wenyonii (strain Massachusetts) OX=1197325 GN=ksgA PE=3 SV=1

MM1 pKa = 7.45SFLPFFQHH9 pKa = 7.57IEE11 pKa = 3.91DD12 pKa = 4.46SFNKK16 pKa = 9.71LWNDD20 pKa = 3.56SISFVFAILGLFCVIAAFWPGFYY43 pKa = 9.56NTWKK47 pKa = 9.66TKK49 pKa = 8.89DD50 pKa = 3.52TEE52 pKa = 4.16FLPIGLFSLFLLVGVLMSAGSIAGICEE79 pKa = 4.18SPKK82 pKa = 10.26LGPMYY87 pKa = 10.27MRR89 pKa = 11.84GCVFTLLNVFVMLTNLYY106 pKa = 8.86TVGLWCKK113 pKa = 10.56NKK115 pKa = 9.69FLGRR119 pKa = 11.84RR120 pKa = 11.84GADD123 pKa = 2.77SSYY126 pKa = 11.4ADD128 pKa = 3.19VSEE131 pKa = 4.03TSEE134 pKa = 4.22YY135 pKa = 11.11VDD137 pKa = 4.8DD138 pKa = 4.29EE139 pKa = 4.51QEE141 pKa = 3.83EE142 pKa = 4.44VEE144 pKa = 4.35YY145 pKa = 11.3NEE147 pKa = 4.9EE148 pKa = 3.95EE149 pKa = 4.28SQEE152 pKa = 4.07EE153 pKa = 4.67SNLMDD158 pKa = 5.22LGLAII163 pKa = 5.12

Molecular weight: 18.47 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|I6Z6G1|I6Z6G1_MYCWM Uncharacterized protein OS=Mycoplasma wenyonii (strain Massachusetts) OX=1197325 GN=WEN_01925 PE=4 SV=1

MM1 pKa = 7.33ARR3 pKa = 11.84KK4 pKa = 9.4ALILKK9 pKa = 6.98QQKK12 pKa = 9.07VPKK15 pKa = 9.71FSTRR19 pKa = 11.84AYY21 pKa = 7.98TRR23 pKa = 11.84CGRR26 pKa = 11.84CGRR29 pKa = 11.84SRR31 pKa = 11.84AVFRR35 pKa = 11.84DD36 pKa = 3.42YY37 pKa = 10.56MLCRR41 pKa = 11.84LCFRR45 pKa = 11.84EE46 pKa = 4.11LASWGYY52 pKa = 10.29IPGVMKK58 pKa = 10.9ASWW61 pKa = 2.94

Molecular weight: 7.16 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
651	0	651	185434	37	2648	284.8	32.49

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
4.537 ± 0.074	1.225 ± 0.035	4.279 ± 0.067	7.993 ± 0.126	5.196 ± 0.073	6.413 ± 0.115	1.441 ± 0.031	6.931 ± 0.085	9.781 ± 0.096	10.482 ± 0.11

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.44 ± 0.032	5.227 ± 0.064	3.067 ± 0.043	3.556 ± 0.051	3.945 ± 0.057	8.502 ± 0.082	5.19 ± 0.063	5.452 ± 0.077	1.757 ± 0.051	3.584 ± 0.066

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski