Synechococcus phage ACG-2014e

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 213 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3HJU1|A0A0E3HJU1_9CAUD Structural protein OS=Synechococcus phage ACG-2014e OX=1493510 GN=Syn7803C2_83 PE=4 SV=1
MM1 pKa = 7.56SNYY4 pKa = 10.34KK5 pKa = 8.71ITDD8 pKa = 3.41DD9 pKa = 3.63SATVDD14 pKa = 3.26RR15 pKa = 11.84LSQQRR20 pKa = 11.84DD21 pKa = 4.13DD22 pKa = 3.93IYY24 pKa = 10.95DD25 pKa = 3.35WCVEE29 pKa = 3.71RR30 pKa = 11.84FRR32 pKa = 11.84YY33 pKa = 9.73HH34 pKa = 6.08MANDD38 pKa = 4.31NIDD41 pKa = 3.67EE42 pKa = 4.48ALALADD48 pKa = 5.58EE49 pKa = 4.92FFEE52 pKa = 4.55WMDD55 pKa = 3.79PDD57 pKa = 4.02GMEE60 pKa = 4.16KK61 pKa = 10.79EE62 pKa = 3.87EE63 pKa = 3.89TAFYY67 pKa = 11.34NEE69 pKa = 4.69DD70 pKa = 3.91DD71 pKa = 4.58LLNLYY76 pKa = 10.64LSLPEE81 pKa = 4.19NYY83 pKa = 10.22GG84 pKa = 3.32

Molecular weight: 10.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3HAZ2|A0A0E3HAZ2_9CAUD Long tail fiber protein p37 OS=Synechococcus phage ACG-2014e OX=1493510 GN=Syn7803C2_177 PE=3 SV=1
MM1 pKa = 7.56AKK3 pKa = 10.11SKK5 pKa = 10.95VGLSGGDD12 pKa = 3.64TIEE15 pKa = 4.3SRR17 pKa = 11.84PKK19 pKa = 9.27RR20 pKa = 11.84TRR22 pKa = 11.84QGRR25 pKa = 11.84GKK27 pKa = 7.67HH28 pKa = 4.76TKK30 pKa = 8.41YY31 pKa = 9.54TPTSRR36 pKa = 11.84NSAKK40 pKa = 9.94KK41 pKa = 9.68RR42 pKa = 11.84YY43 pKa = 8.7RR44 pKa = 11.84GQGRR48 pKa = 11.84GG49 pKa = 3.22

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

213

0

213

59610

38

4075

279.9

30.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.751 ± 0.245

0.847 ± 0.079

6.667 ± 0.135

5.828 ± 0.312

4.34 ± 0.122

8.17 ± 0.424

1.354 ± 0.136

6.427 ± 0.196

5.419 ± 0.374

7.106 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.926 ± 0.184

6.217 ± 0.193

3.986 ± 0.11

3.56 ± 0.102

3.748 ± 0.119

7.611 ± 0.267

7.95 ± 0.379

6.72 ± 0.16

1.146 ± 0.086

4.229 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski