Proteome-pI 2.0 - Proteome Isoelectric Point Database

Luteibacter sp. UNCMF366Tsu5.1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Luteibacter; unclassified Luteibacter

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3582 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1K1RPS4|A0A1K1RPS4_9GAMM Type IV pilus assembly protein PilO OS=Luteibacter sp. UNCMF366Tsu5.1 OX=1502758 GN=SAMN02800691_3356 PE=4 SV=1

MM1 pKa = 6.51TQSTEE6 pKa = 3.61TTLRR10 pKa = 11.84KK11 pKa = 8.78WMCVVCGFIYY21 pKa = 10.65DD22 pKa = 3.97EE23 pKa = 5.19AEE25 pKa = 4.0GLPDD29 pKa = 3.62EE30 pKa = 5.37GIEE33 pKa = 4.63PGTRR37 pKa = 11.84WEE39 pKa = 5.53DD40 pKa = 3.72IPDD43 pKa = 3.14TWTCPDD49 pKa = 3.91CGVTKK54 pKa = 10.7DD55 pKa = 3.65DD56 pKa = 4.25FEE58 pKa = 4.42MVEE61 pKa = 4.08LL62 pKa = 4.49

Molecular weight: 7.09 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1K1NYS9|A0A1K1NYS9_9GAMM Glycine cleavage system H protein OS=Luteibacter sp. UNCMF366Tsu5.1 OX=1502758 GN=gcvH PE=3 SV=1

MM1 pKa = 7.24ATAKK5 pKa = 10.46KK6 pKa = 9.08ATAKK10 pKa = 10.48KK11 pKa = 9.91SAAKK15 pKa = 10.17KK16 pKa = 8.13ATKK19 pKa = 10.16KK20 pKa = 9.51STVKK24 pKa = 9.39KK25 pKa = 7.6TAARR29 pKa = 11.84KK30 pKa = 9.77SPAKK34 pKa = 9.94KK35 pKa = 10.08ASTRR39 pKa = 11.84KK40 pKa = 9.56VATKK44 pKa = 10.14KK45 pKa = 10.21AATRR49 pKa = 11.84KK50 pKa = 10.05SPAKK54 pKa = 9.73KK55 pKa = 9.86AAAKK59 pKa = 9.89KK60 pKa = 9.38VARR63 pKa = 11.84KK64 pKa = 9.79APARR68 pKa = 11.84KK69 pKa = 8.88VAAKK73 pKa = 10.09KK74 pKa = 9.95AVKK77 pKa = 10.14KK78 pKa = 10.31AVKK81 pKa = 10.06KK82 pKa = 9.61ATAKK86 pKa = 10.29KK87 pKa = 8.35ATRR90 pKa = 11.84KK91 pKa = 9.71ASPRR95 pKa = 11.84KK96 pKa = 8.95AASKK100 pKa = 10.22KK101 pKa = 9.12VAATKK106 pKa = 10.06VARR109 pKa = 11.84ATKK112 pKa = 9.71AAKK115 pKa = 10.23AKK117 pKa = 10.49

Molecular weight: 12.1 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3582	0	3582	1261826	39	4280	352.3	38.25

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
12.653 ± 0.063	0.762 ± 0.012	6.135 ± 0.033	5.164 ± 0.04	3.488 ± 0.025	8.511 ± 0.04	2.364 ± 0.02	4.281 ± 0.023	2.935 ± 0.033	10.182 ± 0.055

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.261 ± 0.019	2.605 ± 0.033	5.297 ± 0.034	3.255 ± 0.027	7.435 ± 0.05	5.46 ± 0.035	5.473 ± 0.043	7.797 ± 0.032	1.5 ± 0.015	2.442 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski