Lake Sarah-associated circular virus-35

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQQ3|A0A140AQQ3_9VIRU Coat protein OS=Lake Sarah-associated circular virus-35 OX=1685763 PE=4 SV=1
MM1 pKa = 7.28YY2 pKa = 9.91APPEE6 pKa = 3.98ISTIQPPQAEE16 pKa = 4.24LCYY19 pKa = 10.06YY20 pKa = 10.45TMLSQLPGMKK30 pKa = 9.64TYY32 pKa = 10.79DD33 pKa = 3.55ILFGDD38 pKa = 5.02LDD40 pKa = 3.92TANFFPLLDD49 pKa = 5.43GITQGTGPHH58 pKa = 5.13QRR60 pKa = 11.84VGTSILLHH68 pKa = 6.15GIEE71 pKa = 4.14MRR73 pKa = 11.84FFTKK77 pKa = 10.61LQDD80 pKa = 3.57NEE82 pKa = 3.92QDD84 pKa = 3.66VVSFNARR91 pKa = 11.84FILVRR96 pKa = 11.84SLAGSKK102 pKa = 10.0PLRR105 pKa = 11.84DD106 pKa = 3.95DD107 pKa = 3.44VLEE110 pKa = 4.31GLVSSMIAHH119 pKa = 6.04YY120 pKa = 10.92NSDD123 pKa = 4.23FVPDD127 pKa = 4.48LYY129 pKa = 11.33EE130 pKa = 3.99PFYY133 pKa = 11.25DD134 pKa = 3.85EE135 pKa = 5.76YY136 pKa = 10.74IPEE139 pKa = 4.39MYY141 pKa = 9.76VQNPVLPVQSYY152 pKa = 9.35RR153 pKa = 11.84NFYY156 pKa = 10.75RR157 pKa = 11.84SFPVSWRR164 pKa = 11.84VVYY167 pKa = 10.0PRR169 pKa = 11.84DD170 pKa = 3.5NMDD173 pKa = 3.46PQKK176 pKa = 10.72IAGNVYY182 pKa = 10.57ALNLSDD188 pKa = 4.74FSSSIPAINVRR199 pKa = 11.84FYY201 pKa = 10.53YY202 pKa = 9.98TDD204 pKa = 3.15RR205 pKa = 5.91

Molecular weight: 23.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQQ3|A0A140AQQ3_9VIRU Coat protein OS=Lake Sarah-associated circular virus-35 OX=1685763 PE=4 SV=1
MM1 pKa = 6.96YY2 pKa = 10.19GVRR5 pKa = 11.84KK6 pKa = 9.23EE7 pKa = 4.19VFRR10 pKa = 11.84SAMTTAPARR19 pKa = 11.84RR20 pKa = 11.84PNSTRR25 pKa = 11.84TRR27 pKa = 11.84TRR29 pKa = 11.84VGQRR33 pKa = 11.84LRR35 pKa = 11.84RR36 pKa = 11.84FCFTINNYY44 pKa = 8.63TEE46 pKa = 4.0ALYY49 pKa = 11.03DD50 pKa = 3.74EE51 pKa = 5.19LLAFAVTTSWFVVGKK66 pKa = 9.93EE67 pKa = 3.78VGEE70 pKa = 4.25NGTPHH75 pKa = 6.6LQGACILNNQLSFNSLKK92 pKa = 11.0KK93 pKa = 9.06MDD95 pKa = 4.37CFARR99 pKa = 11.84AHH101 pKa = 6.26IEE103 pKa = 4.11TMKK106 pKa = 10.88GSPQDD111 pKa = 3.47NLVYY115 pKa = 9.52CTKK118 pKa = 10.32EE119 pKa = 3.61DD120 pKa = 3.57TNAYY124 pKa = 10.23VYY126 pKa = 8.75GTLPEE131 pKa = 4.58PGKK134 pKa = 11.09RR135 pKa = 11.84NDD137 pKa = 3.08LHH139 pKa = 6.38EE140 pKa = 4.57AVEE143 pKa = 4.35KK144 pKa = 10.6VKK146 pKa = 10.95EE147 pKa = 4.12GATLRR152 pKa = 11.84DD153 pKa = 3.5LALGDD158 pKa = 3.79GGPAVVKK165 pKa = 8.13FHH167 pKa = 7.24KK168 pKa = 10.49GLTVLRR174 pKa = 11.84SLCSTPRR181 pKa = 11.84ADD183 pKa = 3.68PPTVVWIYY191 pKa = 11.17GPTGVHH197 pKa = 5.49KK198 pKa = 9.05TRR200 pKa = 11.84CALEE204 pKa = 3.76FGKK207 pKa = 10.48ILGAASGSSEE217 pKa = 4.25ANSVWISGGGFRR229 pKa = 11.84WFDD232 pKa = 3.68GYY234 pKa = 11.15DD235 pKa = 3.63GQPVAIFDD243 pKa = 4.08DD244 pKa = 5.09FRR246 pKa = 11.84AKK248 pKa = 10.36QLTGAGGFTFLLRR261 pKa = 11.84LLDD264 pKa = 4.36RR265 pKa = 11.84YY266 pKa = 9.72PISVEE271 pKa = 3.99FKK273 pKa = 10.27GGFVNWTPRR282 pKa = 11.84IIFITTPHH290 pKa = 6.69DD291 pKa = 3.56VATTFAKK298 pKa = 10.35RR299 pKa = 11.84SQHH302 pKa = 5.57IPEE305 pKa = 5.93DD306 pKa = 3.24IAQLDD311 pKa = 3.5RR312 pKa = 11.84RR313 pKa = 11.84INKK316 pKa = 9.07VIHH319 pKa = 5.91FPEE322 pKa = 4.62VPSDD326 pKa = 3.65AVKK329 pKa = 11.1LEE331 pKa = 4.16LLDD334 pKa = 5.49ALTLCAGLRR343 pKa = 11.84PAGPQVPATIPGWNEE358 pKa = 3.52DD359 pKa = 3.67VEE361 pKa = 4.69LSGTASDD368 pKa = 5.04GEE370 pKa = 4.32

Molecular weight: 40.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

575

205

370

287.5

32.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.957 ± 1.519

1.391 ± 0.535

6.087 ± 0.728

4.87 ± 0.572

5.739 ± 0.357

7.652 ± 1.642

1.913 ± 0.266

4.87 ± 0.582

3.826 ± 1.11

8.87 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.913 ± 0.889

4.174 ± 0.417

6.783 ± 0.894

3.304 ± 0.931

6.261 ± 0.53

5.913 ± 1.12

6.783 ± 1.416

7.304 ± 0.008

1.217 ± 0.432

4.174 ± 1.86
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski