Halioglobus lutimaris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Halioglobus

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N5X847|A0A2N5X847_9GAMM N-acetyltransferase domain-containing protein OS=Halioglobus lutimaris OX=1737061 GN=C0039_00520 PE=4 SV=1
MM1 pKa = 7.29SVNLIVANDD10 pKa = 3.47DD11 pKa = 4.05SFFHH15 pKa = 6.65EE16 pKa = 4.23ARR18 pKa = 11.84SIKK21 pKa = 10.22EE22 pKa = 4.01NNTEE26 pKa = 3.71EE27 pKa = 6.08AIMFKK32 pKa = 10.84PEE34 pKa = 3.95FKK36 pKa = 10.21LTLLSAAISTFIPLNLADD54 pKa = 4.59AATGCEE60 pKa = 3.79NSARR64 pKa = 11.84LMMRR68 pKa = 11.84SCVAEE73 pKa = 3.93VRR75 pKa = 11.84EE76 pKa = 4.24EE77 pKa = 4.06LTATLAACQSEE88 pKa = 4.86SNNAIRR94 pKa = 11.84KK95 pKa = 7.49ACRR98 pKa = 11.84SAAQEE103 pKa = 4.04EE104 pKa = 4.65VAEE107 pKa = 4.98ARR109 pKa = 11.84DD110 pKa = 3.55TCQEE114 pKa = 3.32QRR116 pKa = 11.84AARR119 pKa = 11.84RR120 pKa = 11.84DD121 pKa = 3.41ACDD124 pKa = 3.32EE125 pKa = 4.25LNEE128 pKa = 4.89DD129 pKa = 4.65RR130 pKa = 11.84YY131 pKa = 11.52DD132 pKa = 3.97DD133 pKa = 4.37PLVDD137 pKa = 4.11GSIKK141 pKa = 10.48FVDD144 pKa = 4.17PDD146 pKa = 5.04DD147 pKa = 6.15IGDD150 pKa = 3.66TWPNNPYY157 pKa = 10.58VILQAGHH164 pKa = 4.9THH166 pKa = 5.77VLAAEE171 pKa = 4.16DD172 pKa = 4.51EE173 pKa = 4.56IVVVYY178 pKa = 8.83ATEE181 pKa = 4.61EE182 pKa = 3.52IRR184 pKa = 11.84EE185 pKa = 4.17IQGVPCRR192 pKa = 11.84VVADD196 pKa = 3.43VVVEE200 pKa = 3.88EE201 pKa = 5.04EE202 pKa = 4.16YY203 pKa = 11.2DD204 pKa = 3.84DD205 pKa = 6.1EE206 pKa = 4.77EE207 pKa = 5.41GEE209 pKa = 3.87WDD211 pKa = 3.56YY212 pKa = 11.8TPIEE216 pKa = 4.27VTDD219 pKa = 3.57DD220 pKa = 3.07WFAQDD225 pKa = 3.55EE226 pKa = 4.45NSNVYY231 pKa = 10.31YY232 pKa = 10.39CGEE235 pKa = 3.87VAQNFEE241 pKa = 4.65DD242 pKa = 4.64GVLRR246 pKa = 11.84DD247 pKa = 3.8LDD249 pKa = 3.88GSFEE253 pKa = 4.33SGLDD257 pKa = 3.36RR258 pKa = 11.84AKK260 pKa = 11.09GGLLTLAMPSPGDD273 pKa = 3.24IHH275 pKa = 6.91RR276 pKa = 11.84QEE278 pKa = 4.29YY279 pKa = 10.12FLGEE283 pKa = 4.16AEE285 pKa = 6.15DD286 pKa = 3.56IVQYY290 pKa = 11.19LDD292 pKa = 4.71LSASPSEE299 pKa = 4.26EE300 pKa = 3.42EE301 pKa = 4.09GGEE304 pKa = 4.07NEE306 pKa = 5.58AYY308 pKa = 9.27PCQGNCLKK316 pKa = 10.04TFDD319 pKa = 4.38FAPLEE324 pKa = 4.45PEE326 pKa = 3.78STEE329 pKa = 3.71YY330 pKa = 10.58KK331 pKa = 10.52YY332 pKa = 10.9YY333 pKa = 10.96LPGTGFVLAVSLEE346 pKa = 4.17DD347 pKa = 4.08GEE349 pKa = 4.22VDD351 pKa = 3.4EE352 pKa = 5.38EE353 pKa = 4.45GRR355 pKa = 11.84EE356 pKa = 4.18SLVCAGPSLEE366 pKa = 4.34VLFDD370 pKa = 4.08DD371 pKa = 4.79ACGIEE376 pKa = 4.55DD377 pKa = 4.41PEE379 pKa = 4.18EE380 pKa = 4.12LMEE383 pKa = 5.47DD384 pKa = 2.89LCEE387 pKa = 4.11FHH389 pKa = 7.74NEE391 pKa = 3.68FCEE394 pKa = 5.03DD395 pKa = 4.61DD396 pKa = 3.85EE397 pKa = 6.75DD398 pKa = 4.54GQQ400 pKa = 4.23

Molecular weight: 44.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N5X8V7|A0A2N5X8V7_9GAMM SPOR domain-containing protein OS=Halioglobus lutimaris OX=1737061 GN=C0039_02030 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.87RR14 pKa = 11.84THH16 pKa = 5.99GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.44GGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84GKK37 pKa = 9.75GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4080

0

4080

1394984

25

2139

341.9

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.493 ± 0.041

1.069 ± 0.014

5.905 ± 0.034

6.438 ± 0.035

3.671 ± 0.023

8.007 ± 0.034

2.182 ± 0.017

5.027 ± 0.028

3.262 ± 0.03

10.548 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.017

3.176 ± 0.024

4.673 ± 0.023

4.082 ± 0.023

6.338 ± 0.031

6.101 ± 0.028

5.033 ± 0.021

7.229 ± 0.033

1.398 ± 0.017

2.822 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski