Bacteroides sp. CAG:1060

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1576 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5BT18|R5BT18_9BACE Uncharacterized protein OS=Bacteroides sp. CAG:1060 OX=1262734 GN=BN459_00592 PE=4 SV=1
MM1 pKa = 7.55LFLEE5 pKa = 5.41GFQPVLMAGLQSLARR20 pKa = 11.84LNLNSNLCVSDD31 pKa = 3.65NGIDD35 pKa = 3.77FLVIVCVPIGDD46 pKa = 4.44FLTATVIILADD57 pKa = 3.41YY58 pKa = 7.94ATYY61 pKa = 9.0LTEE64 pKa = 5.02SKK66 pKa = 10.64AADD69 pKa = 3.46SASVSEE75 pKa = 4.32GLGAA79 pKa = 4.72

Molecular weight: 8.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5BU54|R5BU54_9BACE PfkB domain-containing protein OS=Bacteroides sp. CAG:1060 OX=1262734 GN=BN459_00084 PE=4 SV=1
MM1 pKa = 7.84PNGKK5 pKa = 8.55KK6 pKa = 9.69HH7 pKa = 6.05KK8 pKa = 7.67RR9 pKa = 11.84HH10 pKa = 6.03KK11 pKa = 10.31MSTHH15 pKa = 5.04KK16 pKa = 10.27RR17 pKa = 11.84KK18 pKa = 9.86KK19 pKa = 8.87RR20 pKa = 11.84LRR22 pKa = 11.84LNRR25 pKa = 11.84HH26 pKa = 5.05KK27 pKa = 10.84KK28 pKa = 9.26KK29 pKa = 10.89

Molecular weight: 3.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1576

0

1576

516703

29

1708

327.9

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.749 ± 0.06

1.472 ± 0.024

5.989 ± 0.045

6.326 ± 0.058

4.509 ± 0.038

7.391 ± 0.053

1.823 ± 0.026

6.609 ± 0.057

6.185 ± 0.053

8.839 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.029

4.191 ± 0.048

3.849 ± 0.032

2.747 ± 0.027

4.948 ± 0.049

7.163 ± 0.065

5.106 ± 0.047

7.094 ± 0.054

1.178 ± 0.024

4.081 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski