Streptomyces phage TP1604

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Woodruffvirus; Streptomyces virus TP1604

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3M1L1|A0A0E3M1L1_9CAUD Uncharacterized protein OS=Streptomyces phage TP1604 OX=1636184 GN=SEA_TP1604_47 PE=4 SV=1
MM1 pKa = 8.05PDD3 pKa = 4.26DD4 pKa = 5.47LDD6 pKa = 3.7PTACVDD12 pKa = 3.46AEE14 pKa = 4.18GFEE17 pKa = 4.56WPEE20 pKa = 3.11HH21 pKa = 6.94DD22 pKa = 4.68YY23 pKa = 11.87GHH25 pKa = 6.42TEE27 pKa = 3.98CSRR30 pKa = 11.84CGAEE34 pKa = 4.54APP36 pKa = 3.97

Molecular weight: 3.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3M325|A0A0E3M325_9CAUD Uncharacterized protein OS=Streptomyces phage TP1604 OX=1636184 GN=SEA_TP1604_68 PE=4 SV=1
MM1 pKa = 7.28TATATATAAKK11 pKa = 9.98FEE13 pKa = 4.42TGDD16 pKa = 3.94VVWGRR21 pKa = 11.84PVRR24 pKa = 11.84QGKK27 pKa = 8.62AQARR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 10.41GIVLGLFATDD43 pKa = 3.9PNQLVVWWFGQGAAGMDD60 pKa = 3.54TTTLAFARR68 pKa = 11.84EE69 pKa = 4.17LTKK72 pKa = 11.08SGDD75 pKa = 3.62IFDD78 pKa = 4.28MGAVQAAKK86 pKa = 10.2LARR89 pKa = 11.84GCYY92 pKa = 9.22RR93 pKa = 11.84YY94 pKa = 10.14EE95 pKa = 3.99RR96 pKa = 11.84AHH98 pKa = 5.58SVGRR102 pKa = 11.84MLEE105 pKa = 3.82RR106 pKa = 11.84HH107 pKa = 5.28ARR109 pKa = 11.84RR110 pKa = 11.84MKK112 pKa = 10.74SLGASFPAA120 pKa = 4.73

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

17433

36

1192

245.5

26.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.675 ± 0.477

1.623 ± 0.273

6.47 ± 0.293

5.174 ± 0.234

2.725 ± 0.181

9.046 ± 0.457

1.962 ± 0.187

3.534 ± 0.245

3.012 ± 0.236

8.335 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.133

2.719 ± 0.191

5.708 ± 0.293

3.224 ± 0.105

6.924 ± 0.305

5.231 ± 0.205

6.861 ± 0.221

7.939 ± 0.199

1.623 ± 0.109

1.962 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski