Halanaerobium salsuginis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halanaerobium

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2783 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4FPU0|A0A1I4FPU0_9FIRM D-beta-D-heptose 1-phosphate adenylyltransferase OS=Halanaerobium salsuginis OX=29563 GN=SAMN02983006_00432 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.49KK3 pKa = 9.03LTITIALLLVVAFAMPVFGASFSDD27 pKa = 3.87VPSDD31 pKa = 2.76HH32 pKa = 6.84WAYY35 pKa = 11.05SAINKK40 pKa = 8.93LVAAGIVEE48 pKa = 4.84GYY50 pKa = 9.77PDD52 pKa = 4.63GEE54 pKa = 4.4FKK56 pKa = 10.87GQQSMTRR63 pKa = 11.84YY64 pKa = 8.92EE65 pKa = 4.09MAVMVSRR72 pKa = 11.84ALDD75 pKa = 3.99NIADD79 pKa = 4.09EE80 pKa = 4.55QKK82 pKa = 11.09EE83 pKa = 4.11MAAGLTTGQAEE94 pKa = 4.49DD95 pKa = 3.19VTAIVKK101 pKa = 10.51ALMEE105 pKa = 4.83KK106 pKa = 8.95NTSEE110 pKa = 4.34EE111 pKa = 4.0FTDD114 pKa = 3.78AQAKK118 pKa = 9.2KK119 pKa = 10.48VADD122 pKa = 3.41IVDD125 pKa = 3.58ALTFEE130 pKa = 5.04LKK132 pKa = 10.91AEE134 pKa = 4.24LKK136 pKa = 10.72VLGAKK141 pKa = 9.81VDD143 pKa = 3.89TLGKK147 pKa = 10.43DD148 pKa = 3.06VDD150 pKa = 4.51EE151 pKa = 5.07IKK153 pKa = 10.44ATIAALDD160 pKa = 3.74VPEE163 pKa = 5.13DD164 pKa = 3.84NIEE167 pKa = 3.94FSGVVTTYY175 pKa = 11.11AQVGSYY181 pKa = 11.24GDD183 pKa = 4.73DD184 pKa = 3.64DD185 pKa = 4.2EE186 pKa = 4.73QAATAEE192 pKa = 4.14ALADD196 pKa = 4.09GDD198 pKa = 4.64VLDD201 pKa = 6.01ADD203 pKa = 4.76LSNNGGGDD211 pKa = 3.61YY212 pKa = 11.03DD213 pKa = 5.36DD214 pKa = 6.22FPAEE218 pKa = 3.52QAFYY222 pKa = 11.2QEE224 pKa = 4.83IDD226 pKa = 3.38LLLNGSLKK234 pKa = 10.89GINFDD239 pKa = 4.61LAVDD243 pKa = 4.62TITNGFTDD251 pKa = 4.11YY252 pKa = 11.25EE253 pKa = 4.7GYY255 pKa = 11.01NYY257 pKa = 10.74DD258 pKa = 5.23DD259 pKa = 3.63YY260 pKa = 11.77TKK262 pKa = 11.05VLGEE266 pKa = 4.01QGDD269 pKa = 4.09EE270 pKa = 4.17NGIDD274 pKa = 3.73MDD276 pKa = 4.47TALLTITQDD285 pKa = 2.46NWTVKK290 pKa = 10.09IGDD293 pKa = 3.68MGDD296 pKa = 3.44YY297 pKa = 10.98DD298 pKa = 4.67VDD300 pKa = 3.92DD301 pKa = 3.56SAYY304 pKa = 10.51FYY306 pKa = 10.96DD307 pKa = 4.13VEE309 pKa = 4.26DD310 pKa = 4.15AEE312 pKa = 5.39GIEE315 pKa = 4.51FTGPVYY321 pKa = 10.96GFGVKK326 pKa = 10.09AFAVGYY332 pKa = 10.55DD333 pKa = 4.03DD334 pKa = 5.92GNLDD338 pKa = 4.04EE339 pKa = 5.74FATSDD344 pKa = 3.36DD345 pKa = 4.0LYY347 pKa = 11.34YY348 pKa = 11.06GVAVSKK354 pKa = 10.86EE355 pKa = 3.85FMNNTVVTGKK365 pKa = 10.51VYY367 pKa = 10.58QADD370 pKa = 4.05LDD372 pKa = 4.15SADD375 pKa = 4.12EE376 pKa = 4.27KK377 pKa = 10.82VTDD380 pKa = 3.42IAVAVSTDD388 pKa = 3.45LTDD391 pKa = 3.77SLTLSGEE398 pKa = 3.99AVYY401 pKa = 11.23NEE403 pKa = 4.63LDD405 pKa = 3.67EE406 pKa = 6.74ADD408 pKa = 4.26TDD410 pKa = 4.01DD411 pKa = 5.74SMFKK415 pKa = 10.2IAANYY420 pKa = 9.85IYY422 pKa = 10.32TDD424 pKa = 3.52TLSFRR429 pKa = 11.84GSFQTVGDD437 pKa = 4.11EE438 pKa = 4.33FNGGVDD444 pKa = 3.56HH445 pKa = 7.0FADD448 pKa = 3.53VQADD452 pKa = 3.67MEE454 pKa = 4.41EE455 pKa = 5.48DD456 pKa = 3.7YY457 pKa = 11.72DD458 pKa = 4.13FDD460 pKa = 5.35KK461 pKa = 10.92YY462 pKa = 9.68TLGTTYY468 pKa = 10.73VVNTNNSLDD477 pKa = 3.63FDD479 pKa = 4.34VAMVSYY485 pKa = 10.83DD486 pKa = 3.36ADD488 pKa = 3.73TAFSQDD494 pKa = 3.14FVDD497 pKa = 4.65DD498 pKa = 4.85LGYY501 pKa = 11.06SSDD504 pKa = 3.72TDD506 pKa = 3.4EE507 pKa = 5.37DD508 pKa = 4.18KK509 pKa = 11.37YY510 pKa = 11.0IYY512 pKa = 10.45SIGWNNTYY520 pKa = 10.97GKK522 pKa = 8.64FTNHH526 pKa = 5.59VGLEE530 pKa = 4.19YY531 pKa = 11.09VEE533 pKa = 4.05NDD535 pKa = 3.48YY536 pKa = 10.07YY537 pKa = 11.18TDD539 pKa = 3.57EE540 pKa = 4.58TDD542 pKa = 4.94DD543 pKa = 3.6NTTVIEE549 pKa = 4.62LGTVYY554 pKa = 10.91DD555 pKa = 3.92WTAKK559 pKa = 9.29TDD561 pKa = 3.6LRR563 pKa = 11.84AKK565 pKa = 9.91IVNKK569 pKa = 10.36NADD572 pKa = 3.93LYY574 pKa = 11.36DD575 pKa = 4.44NSDD578 pKa = 3.25VWDD581 pKa = 3.48KK582 pKa = 11.96DD583 pKa = 3.33MNFTYY588 pKa = 10.36LQAGIDD594 pKa = 3.66HH595 pKa = 6.34QLADD599 pKa = 4.47NISWITDD606 pKa = 3.24VQYY609 pKa = 9.93ITGDD613 pKa = 3.04VDD615 pKa = 3.43QLTNHH620 pKa = 6.37SADD623 pKa = 4.07DD624 pKa = 4.0SVDD627 pKa = 3.6SYY629 pKa = 11.67VANGDD634 pKa = 3.59NADD637 pKa = 3.46INGNFITTQLSVSFF651 pKa = 4.46

Molecular weight: 71.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4LIS9|A0A1I4LIS9_9FIRM Uncharacterized protein OS=Halanaerobium salsuginis OX=29563 GN=SAMN02983006_02333 PE=4 SV=1
MM1 pKa = 7.35ARR3 pKa = 11.84IEE5 pKa = 4.74GVDD8 pKa = 3.49LPRR11 pKa = 11.84NKK13 pKa = 9.85RR14 pKa = 11.84VEE16 pKa = 3.72IGLTYY21 pKa = 10.42IFGIGKK27 pKa = 8.41TSAQEE32 pKa = 3.61ILANTGVDD40 pKa = 3.31PDD42 pKa = 4.11TRR44 pKa = 11.84IKK46 pKa = 10.97DD47 pKa = 3.49LTEE50 pKa = 4.7AEE52 pKa = 4.18ISSLRR57 pKa = 11.84SEE59 pKa = 3.86IDD61 pKa = 2.69ANYY64 pKa = 9.6IVEE67 pKa = 4.35GEE69 pKa = 3.95LRR71 pKa = 11.84RR72 pKa = 11.84EE73 pKa = 3.44RR74 pKa = 11.84RR75 pKa = 11.84ADD77 pKa = 3.09IKK79 pKa = 10.57RR80 pKa = 11.84LKK82 pKa = 10.77DD83 pKa = 2.99IGCYY87 pKa = 9.37RR88 pKa = 11.84GLRR91 pKa = 11.84HH92 pKa = 6.72RR93 pKa = 11.84RR94 pKa = 11.84GLPVRR99 pKa = 11.84GQRR102 pKa = 11.84TKK104 pKa = 10.45TNARR108 pKa = 11.84TRR110 pKa = 11.84KK111 pKa = 8.76GPKK114 pKa = 8.14RR115 pKa = 11.84TVGVSRR121 pKa = 11.84VKK123 pKa = 10.8

Molecular weight: 13.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2783

0

2783

888209

39

1537

319.2

35.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.847 ± 0.054

0.662 ± 0.013

5.304 ± 0.038

7.007 ± 0.052

4.535 ± 0.037

6.256 ± 0.04

1.467 ± 0.016

8.649 ± 0.047

7.771 ± 0.049

10.697 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.022

5.539 ± 0.045

3.209 ± 0.024

3.95 ± 0.034

3.652 ± 0.024

5.968 ± 0.032

4.698 ± 0.029

5.972 ± 0.035

0.817 ± 0.017

3.811 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski