Proteome-pI 2.0 - Proteome Isoelectric Point Database

Hubei narna-like virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1L3KIB1|A0A1L3KIB1_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 15 OX=1922945 PE=4 SV=1

MM1 pKa = 7.62ALTYY5 pKa = 8.14EE6 pKa = 3.88LCMPVFNGDD15 pKa = 3.5ALRR18 pKa = 11.84VGSIVNFFCRR28 pKa = 11.84RR29 pKa = 11.84SCTYY33 pKa = 9.75PARR36 pKa = 11.84QSDD39 pKa = 4.4GPKK42 pKa = 9.94CPKK45 pKa = 10.51DD46 pKa = 3.9FISRR50 pKa = 11.84EE51 pKa = 4.01ILKK54 pKa = 10.77SSLPHH59 pKa = 5.82FRR61 pKa = 11.84VPGPACSHH69 pKa = 6.28KK70 pKa = 10.42LRR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84LAQTLNAFAAAFISVWPSSQFGLCPTPPNLTAMWALCKK112 pKa = 10.06FLHH115 pKa = 5.96WVCKK119 pKa = 10.03VYY121 pKa = 10.9VGNGEE126 pKa = 3.98PWLAPVLRR134 pKa = 11.84GIATWARR141 pKa = 11.84YY142 pKa = 7.98HH143 pKa = 7.35ASTTHH148 pKa = 6.05LHH150 pKa = 6.52LPPEE154 pKa = 4.96GIFRR158 pKa = 11.84TPLSSADD165 pKa = 3.33GSIDD169 pKa = 3.44FGKK172 pKa = 10.58LFTGSISRR180 pKa = 11.84KK181 pKa = 7.85ATSPSCLLRR190 pKa = 11.84FSRR193 pKa = 11.84FARR196 pKa = 11.84ALPPGDD202 pKa = 5.0DD203 pKa = 3.44WTCAQGLVDD212 pKa = 3.94HH213 pKa = 6.23QKK215 pKa = 11.36AMRR218 pKa = 11.84DD219 pKa = 3.45NPTVPEE225 pKa = 3.95QLTKK229 pKa = 10.8LFKK232 pKa = 10.84DD233 pKa = 3.28VATRR237 pKa = 11.84ISCQPLLKK245 pKa = 10.71DD246 pKa = 3.18VGTPGFTLSMSSCLEE261 pKa = 3.77RR262 pKa = 11.84TRR264 pKa = 11.84SQGGAAEE271 pKa = 4.17EE272 pKa = 4.11VKK274 pKa = 10.53EE275 pKa = 4.14AVWEE279 pKa = 4.01IFPGLRR285 pKa = 11.84RR286 pKa = 11.84IHH288 pKa = 6.26EE289 pKa = 4.49GVTEE293 pKa = 4.14EE294 pKa = 4.65EE295 pKa = 4.15DD296 pKa = 3.89TEE298 pKa = 4.4LSRR301 pKa = 11.84VPCLDD306 pKa = 3.66RR307 pKa = 11.84NPLYY311 pKa = 11.07SDD313 pKa = 3.29VEE315 pKa = 4.51YY316 pKa = 10.87YY317 pKa = 10.77YY318 pKa = 11.19SFYY321 pKa = 11.03EE322 pKa = 4.06GLPEE326 pKa = 3.81HH327 pKa = 6.41VPRR330 pKa = 11.84VEE332 pKa = 3.73RR333 pKa = 11.84LTYY336 pKa = 9.91LACCEE341 pKa = 4.43KK342 pKa = 10.66VFPPCRR348 pKa = 11.84LEE350 pKa = 3.9RR351 pKa = 11.84RR352 pKa = 11.84VPSHH356 pKa = 7.08RR357 pKa = 11.84IIPVPDD363 pKa = 3.15RR364 pKa = 11.84GGFKK368 pKa = 10.1IRR370 pKa = 11.84VITAGEE376 pKa = 4.12ALTQSLAHH384 pKa = 5.28QVRR387 pKa = 11.84KK388 pKa = 9.6VVYY391 pKa = 9.8RR392 pKa = 11.84KK393 pKa = 9.72VLPTLPTVWGIRR405 pKa = 11.84EE406 pKa = 3.92DD407 pKa = 3.64GVRR410 pKa = 11.84NFIEE414 pKa = 4.3QLRR417 pKa = 11.84MPSSPDD423 pKa = 3.02PRR425 pKa = 11.84LGRR428 pKa = 11.84WVALSCDD435 pKa = 3.45MKK437 pKa = 11.02AATDD441 pKa = 4.19RR442 pKa = 11.84FPHH445 pKa = 6.22PLIEE449 pKa = 5.1AINDD453 pKa = 3.75GLEE456 pKa = 4.4SNLSEE461 pKa = 4.3SQRR464 pKa = 11.84RR465 pKa = 11.84CPAWLAWRR473 pKa = 11.84SLSGPQRR480 pKa = 11.84LLYY483 pKa = 10.15PGGDD487 pKa = 3.4VVLSSCGNLMGTAPSWALLNMYY509 pKa = 10.25NYY511 pKa = 11.33ALFRR515 pKa = 11.84LAWSIWSTGWSRR527 pKa = 11.84RR528 pKa = 11.84KK529 pKa = 9.91FPEE532 pKa = 3.59LAARR536 pKa = 11.84EE537 pKa = 4.19VLPSRR542 pKa = 11.84QSLDD546 pKa = 3.73GSWEE550 pKa = 3.93PLKK553 pKa = 11.21SRR555 pKa = 11.84VLRR558 pKa = 11.84VLSDD562 pKa = 3.57SRR564 pKa = 11.84FHH566 pKa = 6.76PWNFPKK572 pKa = 10.61GLKK575 pKa = 10.16FNEE578 pKa = 3.86LAALVGDD585 pKa = 4.97DD586 pKa = 5.03LAAACPFGVAMLYY599 pKa = 10.51EE600 pKa = 5.46LILEE604 pKa = 4.56LCNGKK609 pKa = 9.47PSAGKK614 pKa = 9.63HH615 pKa = 4.75YY616 pKa = 10.67VMPWIDD622 pKa = 3.65GSYY625 pKa = 10.97LLLAEE630 pKa = 4.28EE631 pKa = 4.57FGFVKK636 pKa = 10.37GDD638 pKa = 3.59RR639 pKa = 11.84LVHH642 pKa = 5.57LHH644 pKa = 7.11AEE646 pKa = 4.6FLRR649 pKa = 11.84GIVTGTNCVDD659 pKa = 3.09TRR661 pKa = 11.84SRR663 pKa = 11.84NPEE666 pKa = 3.4WAQIGLSLAASIAGCRR682 pKa = 11.84NEE684 pKa = 3.71TRR686 pKa = 11.84RR687 pKa = 11.84ALSSFSHH694 pKa = 6.76LMLADD699 pKa = 3.55LRR701 pKa = 11.84KK702 pKa = 7.55TLLRR706 pKa = 11.84FGLPIYY712 pKa = 10.54LPASVGGLGWPHH724 pKa = 6.45PRR726 pKa = 11.84GLEE729 pKa = 3.87YY730 pKa = 10.93ALEE733 pKa = 4.13RR734 pKa = 11.84TSAKK738 pKa = 9.9VLRR741 pKa = 11.84AYY743 pKa = 10.6SVVRR747 pKa = 11.84GFRR750 pKa = 11.84QDD752 pKa = 3.14PVQFILIIARR762 pKa = 11.84LRR764 pKa = 11.84SSWMRR769 pKa = 11.84PEE771 pKa = 4.02LRR773 pKa = 11.84TPFSNLLSLLSGYY786 pKa = 9.41FRR788 pKa = 11.84SLKK791 pKa = 10.12VSRR794 pKa = 11.84DD795 pKa = 3.46EE796 pKa = 4.09QGRR799 pKa = 11.84LAPCTPEE806 pKa = 4.35SPIGLPSLDD815 pKa = 3.35GTSKK819 pKa = 11.04NFFDD823 pKa = 5.91CIDD826 pKa = 4.01SIGVHH831 pKa = 5.6GVTVAFVAGEE841 pKa = 4.04EE842 pKa = 4.52LPLLEE847 pKa = 4.01QKK849 pKa = 10.37EE850 pKa = 4.2RR851 pKa = 11.84FRR853 pKa = 11.84TIGASRR859 pKa = 11.84RR860 pKa = 11.84AYY862 pKa = 9.7GQAISDD868 pKa = 3.82LLRR871 pKa = 11.84RR872 pKa = 11.84RR873 pKa = 11.84EE874 pKa = 3.97GHH876 pKa = 6.15VPPLLPRR883 pKa = 11.84RR884 pKa = 11.84SDD886 pKa = 3.54YY887 pKa = 11.42AALIAEE893 pKa = 4.44DD894 pKa = 3.77EE895 pKa = 4.3KK896 pKa = 11.38LVRR899 pKa = 11.84DD900 pKa = 3.49CHH902 pKa = 7.1LYY904 pKa = 10.75LGEE907 pKa = 4.19SLEE910 pKa = 4.19AQLPFLQGVLGKK922 pKa = 10.3SPEE925 pKa = 4.06EE926 pKa = 3.7ALEE929 pKa = 4.87DD930 pKa = 3.43IGEE933 pKa = 4.33AEE935 pKa = 4.0

Molecular weight: 104.66 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1L3KIB1|A0A1L3KIB1_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 15 OX=1922945 PE=4 SV=1

MM1 pKa = 7.62ALTYY5 pKa = 8.14EE6 pKa = 3.88LCMPVFNGDD15 pKa = 3.5ALRR18 pKa = 11.84VGSIVNFFCRR28 pKa = 11.84RR29 pKa = 11.84SCTYY33 pKa = 9.75PARR36 pKa = 11.84QSDD39 pKa = 4.4GPKK42 pKa = 9.94CPKK45 pKa = 10.51DD46 pKa = 3.9FISRR50 pKa = 11.84EE51 pKa = 4.01ILKK54 pKa = 10.77SSLPHH59 pKa = 5.82FRR61 pKa = 11.84VPGPACSHH69 pKa = 6.28KK70 pKa = 10.42LRR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84LAQTLNAFAAAFISVWPSSQFGLCPTPPNLTAMWALCKK112 pKa = 10.06FLHH115 pKa = 5.96WVCKK119 pKa = 10.03VYY121 pKa = 10.9VGNGEE126 pKa = 3.98PWLAPVLRR134 pKa = 11.84GIATWARR141 pKa = 11.84YY142 pKa = 7.98HH143 pKa = 7.35ASTTHH148 pKa = 6.05LHH150 pKa = 6.52LPPEE154 pKa = 4.96GIFRR158 pKa = 11.84TPLSSADD165 pKa = 3.33GSIDD169 pKa = 3.44FGKK172 pKa = 10.58LFTGSISRR180 pKa = 11.84KK181 pKa = 7.85ATSPSCLLRR190 pKa = 11.84FSRR193 pKa = 11.84FARR196 pKa = 11.84ALPPGDD202 pKa = 5.0DD203 pKa = 3.44WTCAQGLVDD212 pKa = 3.94HH213 pKa = 6.23QKK215 pKa = 11.36AMRR218 pKa = 11.84DD219 pKa = 3.45NPTVPEE225 pKa = 3.95QLTKK229 pKa = 10.8LFKK232 pKa = 10.84DD233 pKa = 3.28VATRR237 pKa = 11.84ISCQPLLKK245 pKa = 10.71DD246 pKa = 3.18VGTPGFTLSMSSCLEE261 pKa = 3.77RR262 pKa = 11.84TRR264 pKa = 11.84SQGGAAEE271 pKa = 4.17EE272 pKa = 4.11VKK274 pKa = 10.53EE275 pKa = 4.14AVWEE279 pKa = 4.01IFPGLRR285 pKa = 11.84RR286 pKa = 11.84IHH288 pKa = 6.26EE289 pKa = 4.49GVTEE293 pKa = 4.14EE294 pKa = 4.65EE295 pKa = 4.15DD296 pKa = 3.89TEE298 pKa = 4.4LSRR301 pKa = 11.84VPCLDD306 pKa = 3.66RR307 pKa = 11.84NPLYY311 pKa = 11.07SDD313 pKa = 3.29VEE315 pKa = 4.51YY316 pKa = 10.87YY317 pKa = 10.77YY318 pKa = 11.19SFYY321 pKa = 11.03EE322 pKa = 4.06GLPEE326 pKa = 3.81HH327 pKa = 6.41VPRR330 pKa = 11.84VEE332 pKa = 3.73RR333 pKa = 11.84LTYY336 pKa = 9.91LACCEE341 pKa = 4.43KK342 pKa = 10.66VFPPCRR348 pKa = 11.84LEE350 pKa = 3.9RR351 pKa = 11.84RR352 pKa = 11.84VPSHH356 pKa = 7.08RR357 pKa = 11.84IIPVPDD363 pKa = 3.15RR364 pKa = 11.84GGFKK368 pKa = 10.1IRR370 pKa = 11.84VITAGEE376 pKa = 4.12ALTQSLAHH384 pKa = 5.28QVRR387 pKa = 11.84KK388 pKa = 9.6VVYY391 pKa = 9.8RR392 pKa = 11.84KK393 pKa = 9.72VLPTLPTVWGIRR405 pKa = 11.84EE406 pKa = 3.92DD407 pKa = 3.64GVRR410 pKa = 11.84NFIEE414 pKa = 4.3QLRR417 pKa = 11.84MPSSPDD423 pKa = 3.02PRR425 pKa = 11.84LGRR428 pKa = 11.84WVALSCDD435 pKa = 3.45MKK437 pKa = 11.02AATDD441 pKa = 4.19RR442 pKa = 11.84FPHH445 pKa = 6.22PLIEE449 pKa = 5.1AINDD453 pKa = 3.75GLEE456 pKa = 4.4SNLSEE461 pKa = 4.3SQRR464 pKa = 11.84RR465 pKa = 11.84CPAWLAWRR473 pKa = 11.84SLSGPQRR480 pKa = 11.84LLYY483 pKa = 10.15PGGDD487 pKa = 3.4VVLSSCGNLMGTAPSWALLNMYY509 pKa = 10.25NYY511 pKa = 11.33ALFRR515 pKa = 11.84LAWSIWSTGWSRR527 pKa = 11.84RR528 pKa = 11.84KK529 pKa = 9.91FPEE532 pKa = 3.59LAARR536 pKa = 11.84EE537 pKa = 4.19VLPSRR542 pKa = 11.84QSLDD546 pKa = 3.73GSWEE550 pKa = 3.93PLKK553 pKa = 11.21SRR555 pKa = 11.84VLRR558 pKa = 11.84VLSDD562 pKa = 3.57SRR564 pKa = 11.84FHH566 pKa = 6.76PWNFPKK572 pKa = 10.61GLKK575 pKa = 10.16FNEE578 pKa = 3.86LAALVGDD585 pKa = 4.97DD586 pKa = 5.03LAAACPFGVAMLYY599 pKa = 10.51EE600 pKa = 5.46LILEE604 pKa = 4.56LCNGKK609 pKa = 9.47PSAGKK614 pKa = 9.63HH615 pKa = 4.75YY616 pKa = 10.67VMPWIDD622 pKa = 3.65GSYY625 pKa = 10.97LLLAEE630 pKa = 4.28EE631 pKa = 4.57FGFVKK636 pKa = 10.37GDD638 pKa = 3.59RR639 pKa = 11.84LVHH642 pKa = 5.57LHH644 pKa = 7.11AEE646 pKa = 4.6FLRR649 pKa = 11.84GIVTGTNCVDD659 pKa = 3.09TRR661 pKa = 11.84SRR663 pKa = 11.84NPEE666 pKa = 3.4WAQIGLSLAASIAGCRR682 pKa = 11.84NEE684 pKa = 3.71TRR686 pKa = 11.84RR687 pKa = 11.84ALSSFSHH694 pKa = 6.76LMLADD699 pKa = 3.55LRR701 pKa = 11.84KK702 pKa = 7.55TLLRR706 pKa = 11.84FGLPIYY712 pKa = 10.54LPASVGGLGWPHH724 pKa = 6.45PRR726 pKa = 11.84GLEE729 pKa = 3.87YY730 pKa = 10.93ALEE733 pKa = 4.13RR734 pKa = 11.84TSAKK738 pKa = 9.9VLRR741 pKa = 11.84AYY743 pKa = 10.6SVVRR747 pKa = 11.84GFRR750 pKa = 11.84QDD752 pKa = 3.14PVQFILIIARR762 pKa = 11.84LRR764 pKa = 11.84SSWMRR769 pKa = 11.84PEE771 pKa = 4.02LRR773 pKa = 11.84TPFSNLLSLLSGYY786 pKa = 9.41FRR788 pKa = 11.84SLKK791 pKa = 10.12VSRR794 pKa = 11.84DD795 pKa = 3.46EE796 pKa = 4.09QGRR799 pKa = 11.84LAPCTPEE806 pKa = 4.35SPIGLPSLDD815 pKa = 3.35GTSKK819 pKa = 11.04NFFDD823 pKa = 5.91CIDD826 pKa = 4.01SIGVHH831 pKa = 5.6GVTVAFVAGEE841 pKa = 4.04EE842 pKa = 4.52LPLLEE847 pKa = 4.01QKK849 pKa = 10.37EE850 pKa = 4.2RR851 pKa = 11.84FRR853 pKa = 11.84TIGASRR859 pKa = 11.84RR860 pKa = 11.84AYY862 pKa = 9.7GQAISDD868 pKa = 3.82LLRR871 pKa = 11.84RR872 pKa = 11.84RR873 pKa = 11.84EE874 pKa = 3.97GHH876 pKa = 6.15VPPLLPRR883 pKa = 11.84RR884 pKa = 11.84SDD886 pKa = 3.54YY887 pKa = 11.42AALIAEE893 pKa = 4.44DD894 pKa = 3.77EE895 pKa = 4.3KK896 pKa = 11.38LVRR899 pKa = 11.84DD900 pKa = 3.49CHH902 pKa = 7.1LYY904 pKa = 10.75LGEE907 pKa = 4.19SLEE910 pKa = 4.19AQLPFLQGVLGKK922 pKa = 10.3SPEE925 pKa = 4.06EE926 pKa = 3.7ALEE929 pKa = 4.87DD930 pKa = 3.43IGEE933 pKa = 4.33AEE935 pKa = 4.0

Molecular weight: 104.66 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	935	935	935	935.0	104.66

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
7.807 ± 0.0	2.781 ± 0.0	4.171 ± 0.0	6.096 ± 0.0	4.492 ± 0.0	7.166 ± 0.0	2.246 ± 0.0	3.85 ± 0.0	3.422 ± 0.0	12.513 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.39 ± 0.0	2.246 ± 0.0	7.487 ± 0.0	2.353 ± 0.0	8.877 ± 0.0	8.128 ± 0.0	4.171 ± 0.0	5.989 ± 0.0	2.246 ± 0.0	2.567 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski