Angelica bushy stunt virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Caulimovirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140GL64|A0A140GL64_9VIRU Uncharacterized protein OS=Angelica bushy stunt virus OX=1808970 PE=4 SV=1
MM1 pKa = 7.95ILEE4 pKa = 4.27IEE6 pKa = 4.42EE7 pKa = 4.56NPNLWIVDD15 pKa = 4.52LDD17 pKa = 3.89PAGIIPMDD25 pKa = 4.15TNNALQFLVRR35 pKa = 11.84AITEE39 pKa = 4.08ALEE42 pKa = 4.27DD43 pKa = 3.93TNNLSRR49 pKa = 11.84LLEE52 pKa = 4.15LNIDD56 pKa = 3.01ISLISRR62 pKa = 11.84RR63 pKa = 11.84NGLLASQYY71 pKa = 10.43ISRR74 pKa = 11.84LFHH77 pKa = 7.1DD78 pKa = 3.69RR79 pKa = 11.84LLRR82 pKa = 11.84RR83 pKa = 11.84IRR85 pKa = 11.84QLQEE89 pKa = 3.27RR90 pKa = 11.84ANALEE95 pKa = 3.92NLEE98 pKa = 4.83NLHH101 pKa = 7.12QDD103 pKa = 3.93FQNQAPIFRR112 pKa = 11.84TEE114 pKa = 4.1DD115 pKa = 3.42DD116 pKa = 3.68QEE118 pKa = 4.37EE119 pKa = 4.49EE120 pKa = 4.27LFDD123 pKa = 3.57PHH125 pKa = 6.77RR126 pKa = 11.84CHH128 pKa = 7.21AFCFCKK134 pKa = 10.71GKK136 pKa = 10.16II137 pKa = 3.57

Molecular weight: 16.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140GL60|A0A140GL60_9VIRU Protein 3 OS=Angelica bushy stunt virus OX=1808970 PE=3 SV=1
MM1 pKa = 7.62SSNPLLQHH9 pKa = 5.03PHH11 pKa = 5.35VYY13 pKa = 10.33KK14 pKa = 10.57KK15 pKa = 11.11GKK17 pKa = 9.42ILNLKK22 pKa = 10.04KK23 pKa = 10.44LDD25 pKa = 3.71EE26 pKa = 4.68GTNKK30 pKa = 9.8PKK32 pKa = 10.3RR33 pKa = 11.84VYY35 pKa = 10.89NFASSSGNITTIQNHH50 pKa = 6.03CNNLNQIVGRR60 pKa = 11.84NFLGIAKK67 pKa = 9.5LLSYY71 pKa = 10.62FGLSKK76 pKa = 10.97DD77 pKa = 3.52PSEE80 pKa = 4.72KK81 pKa = 9.96FSKK84 pKa = 10.73EE85 pKa = 3.36PTVYY89 pKa = 10.99NKK91 pKa = 9.59FRR93 pKa = 11.84NLFSDD98 pKa = 3.75RR99 pKa = 11.84GRR101 pKa = 11.84DD102 pKa = 3.44KK103 pKa = 11.13DD104 pKa = 4.01LQPLLEE110 pKa = 4.04KK111 pKa = 10.2TYY113 pKa = 11.38DD114 pKa = 3.47KK115 pKa = 11.29LSNLDD120 pKa = 3.72NKK122 pKa = 10.3IEE124 pKa = 4.1KK125 pKa = 9.89QDD127 pKa = 3.27IDD129 pKa = 4.12LKK131 pKa = 11.17KK132 pKa = 10.57LINRR136 pKa = 11.84EE137 pKa = 3.7PVSRR141 pKa = 11.84KK142 pKa = 9.35EE143 pKa = 3.68IEE145 pKa = 4.14DD146 pKa = 3.65FSRR149 pKa = 11.84EE150 pKa = 3.59IKK152 pKa = 9.97EE153 pKa = 4.0IKK155 pKa = 9.71EE156 pKa = 3.85KK157 pKa = 10.92LVAILGAA164 pKa = 4.07

Molecular weight: 18.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2491

117

703

355.9

41.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.496 ± 0.607

1.606 ± 0.289

5.299 ± 0.36

8.189 ± 0.889

5.018 ± 0.221

4.095 ± 0.252

2.529 ± 0.403

7.668 ± 0.542

10.237 ± 0.939

8.992 ± 0.692

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.967 ± 0.175

6.744 ± 0.763

4.095 ± 0.314

4.697 ± 0.345

4.175 ± 0.443

6.825 ± 0.468

4.456 ± 0.312

4.777 ± 0.334

0.803 ± 0.298

3.332 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski