Escherichia phage ESSI2_ev239

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Seongnamvirus; Escherichia virus ev239

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FUE9|A0A653FUE9_9CAUD DUF5051 domain-containing protein OS=Escherichia phage ESSI2_ev239 OX=2695847 PE=4 SV=1
MM1 pKa = 6.63KK2 pKa = 10.33QNYY5 pKa = 8.62FSYY8 pKa = 11.07EE9 pKa = 4.01EE10 pKa = 4.05LLMGIFNISDD20 pKa = 3.53EE21 pKa = 4.64LYY23 pKa = 7.68EE24 pKa = 4.12TTDD27 pKa = 3.63FDD29 pKa = 4.7EE30 pKa = 4.57LTMEE34 pKa = 4.54HH35 pKa = 6.84FDD37 pKa = 3.8ISFEE41 pKa = 4.03QFANVVDD48 pKa = 3.94VLLPLTAVVHH58 pKa = 6.18SPLSGRR64 pKa = 11.84NYY66 pKa = 10.38HH67 pKa = 7.0AFLKK71 pKa = 10.86DD72 pKa = 3.74GIAFIKK78 pKa = 10.06TEE80 pKa = 3.83ASAA83 pKa = 3.95

Molecular weight: 9.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FW46|A0A653FW46_9CAUD Tc1 tail completion protein OS=Escherichia phage ESSI2_ev239 OX=2695847 PE=4 SV=1
MM1 pKa = 7.88INGEE5 pKa = 4.12LNKK8 pKa = 10.44EE9 pKa = 4.12QFRR12 pKa = 11.84QLQEE16 pKa = 3.85ALKK19 pKa = 10.97KK20 pKa = 10.54LDD22 pKa = 3.78LPSAKK27 pKa = 9.48RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LLWRR34 pKa = 11.84IAKK37 pKa = 10.19YY38 pKa = 10.41GVEE41 pKa = 3.92AAAKK45 pKa = 9.73RR46 pKa = 11.84NVRR49 pKa = 11.84NQQSPEE55 pKa = 3.74GDD57 pKa = 2.55KK58 pKa = 10.12WQARR62 pKa = 11.84QTRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 10.51GKK69 pKa = 7.75MLRR72 pKa = 11.84NMPKK76 pKa = 10.31LIRR79 pKa = 11.84IRR81 pKa = 11.84EE82 pKa = 4.07MPEE85 pKa = 3.73TEE87 pKa = 4.36SVRR90 pKa = 11.84LYY92 pKa = 10.89LAGGHH97 pKa = 5.3YY98 pKa = 10.56RR99 pKa = 11.84NAKK102 pKa = 9.16GNLPAGVVGYY112 pKa = 8.1VQQNGMSVTVNRR124 pKa = 11.84KK125 pKa = 6.62QVEE128 pKa = 4.04GRR130 pKa = 11.84EE131 pKa = 4.16QGDD134 pKa = 3.5KK135 pKa = 10.42PASLRR140 pKa = 11.84QAKK143 pKa = 9.67RR144 pKa = 11.84LRR146 pKa = 11.84KK147 pKa = 9.35AGYY150 pKa = 8.56KK151 pKa = 9.14VRR153 pKa = 11.84RR154 pKa = 11.84GKK156 pKa = 10.05RR157 pKa = 11.84WRR159 pKa = 11.84KK160 pKa = 8.87PGYY163 pKa = 10.06KK164 pKa = 9.77EE165 pKa = 3.33IQEE168 pKa = 4.09RR169 pKa = 11.84MTARR173 pKa = 11.84QAGLLIRR180 pKa = 11.84ILEE183 pKa = 4.43DD184 pKa = 3.55KK185 pKa = 10.16PVKK188 pKa = 9.99TSWQIDD194 pKa = 3.63LPARR198 pKa = 11.84AFLGIGQDD206 pKa = 4.0DD207 pKa = 4.15FNKK210 pKa = 10.6ALARR214 pKa = 11.84QLQAIGFGWDD224 pKa = 3.18VNAQDD229 pKa = 3.3IRR231 pKa = 11.84GRR233 pKa = 11.84AA234 pKa = 3.2

Molecular weight: 26.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

8927

39

885

241.3

26.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.679 ± 0.429

0.795 ± 0.134

5.299 ± 0.23

6.553 ± 0.432

4.145 ± 0.286

7.382 ± 0.375

1.781 ± 0.207

5.646 ± 0.404

5.489 ± 0.33

8.917 ± 0.394

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.227

4.38 ± 0.229

3.932 ± 0.245

4.223 ± 0.319

5.467 ± 0.447

5.87 ± 0.247

6.419 ± 0.313

6.632 ± 0.338

1.837 ± 0.137

3.013 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski