Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Renibacterium; Renibacterium salmoninarum

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3421 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A9WMG0|RL13_RENSM 50S ribosomal protein L13 OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=rplM PE=3 SV=1
MM1 pKa = 7.23SKK3 pKa = 10.18VVPEE7 pKa = 4.04LRR9 pKa = 11.84YY10 pKa = 9.5SAEE13 pKa = 4.3HH14 pKa = 6.03EE15 pKa = 4.18WVANDD20 pKa = 3.58GALNNGPVTIGVSAVAAEE38 pKa = 3.89ALGDD42 pKa = 3.78IVYY45 pKa = 10.72LDD47 pKa = 3.96LPEE50 pKa = 4.4VGSTITAGEE59 pKa = 4.09SCGEE63 pKa = 4.01IEE65 pKa = 4.39STKK68 pKa = 10.72SVSDD72 pKa = 3.7LYY74 pKa = 11.43APVSGEE80 pKa = 3.61ITEE83 pKa = 4.36INADD87 pKa = 3.54AVADD91 pKa = 3.92PAVVNQDD98 pKa = 3.2PYY100 pKa = 10.09GAGWLFKK107 pKa = 10.7VAVSEE112 pKa = 4.06EE113 pKa = 4.46GPLLSAAEE121 pKa = 4.05YY122 pKa = 10.73AEE124 pKa = 4.39KK125 pKa = 10.91NGAEE129 pKa = 4.12LL130 pKa = 4.27

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9WN31|A9WN31_RENSM GMC family oxidoreductase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1820 PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.34VRR4 pKa = 11.84NSLSALKK11 pKa = 9.8KK12 pKa = 10.3VPGAQIVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84TFVINKK30 pKa = 8.65LNPRR34 pKa = 11.84MKK36 pKa = 10.4ARR38 pKa = 11.84QGG40 pKa = 3.24

Molecular weight: 4.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3421

0

3421

929546

22

2433

271.7

29.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.363 ± 0.068

0.674 ± 0.01

5.291 ± 0.036

5.548 ± 0.054

3.394 ± 0.029

8.475 ± 0.043

1.886 ± 0.02

4.966 ± 0.036

3.271 ± 0.033

10.452 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.018 ± 0.019

2.889 ± 0.027

5.051 ± 0.032

3.731 ± 0.028

6.053 ± 0.043

6.795 ± 0.038

5.555 ± 0.029

8.023 ± 0.037

1.458 ± 0.018

2.107 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski