Klebsiella phage KMI8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; unclassified Drexlerviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NHQ1|A0A5B9NHQ1_9CAUD Putative holin OS=Klebsiella phage KMI8 OX=2601619 GN=KMI8_40 PE=4 SV=1
MM1 pKa = 7.71EE2 pKa = 5.17IVQSFEE8 pKa = 4.33PEE10 pKa = 3.78VMLQLLEE17 pKa = 4.09DD18 pKa = 4.56LYY20 pKa = 11.37RR21 pKa = 11.84GIVCRR26 pKa = 11.84KK27 pKa = 8.2LNEE30 pKa = 4.25RR31 pKa = 11.84GLVPFMDD38 pKa = 4.63CDD40 pKa = 3.78PYY42 pKa = 11.53EE43 pKa = 4.33FATSLDD49 pKa = 3.56MSFEE53 pKa = 4.67EE54 pKa = 4.82IMEE57 pKa = 4.5HH58 pKa = 7.08PLCNWDD64 pKa = 4.63AIIDD68 pKa = 3.96FDD70 pKa = 4.94LDD72 pKa = 3.76EE73 pKa = 4.52YY74 pKa = 11.39EE75 pKa = 6.26KK76 pKa = 11.0DD77 pKa = 3.07ILPNGTNCC85 pKa = 4.83

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NHN5|A0A5B9NHN5_9CAUD Uncharacterized protein OS=Klebsiella phage KMI8 OX=2601619 GN=KMI8_78 PE=4 SV=1
MM1 pKa = 7.38VDD3 pKa = 3.74KK4 pKa = 10.52RR5 pKa = 11.84TLNGNNGTQRR15 pKa = 11.84GEE17 pKa = 3.96DD18 pKa = 3.02KK19 pKa = 10.77KK20 pKa = 10.32PRR22 pKa = 11.84KK23 pKa = 9.46RR24 pKa = 11.84RR25 pKa = 11.84TGYY28 pKa = 10.35YY29 pKa = 8.87VLKK32 pKa = 10.75DD33 pKa = 3.58EE34 pKa = 4.52VRR36 pKa = 11.84AGLRR40 pKa = 11.84ARR42 pKa = 11.84LDD44 pKa = 3.48AVFEE48 pKa = 4.46YY49 pKa = 11.0YY50 pKa = 9.04GTQAEE55 pKa = 4.31MARR58 pKa = 11.84RR59 pKa = 11.84LKK61 pKa = 9.47VTYY64 pKa = 9.82QALQNWKK71 pKa = 9.08KK72 pKa = 10.04NGMISVHH79 pKa = 5.62GAMAVHH85 pKa = 7.17RR86 pKa = 11.84DD87 pKa = 3.6YY88 pKa = 11.76VRR90 pKa = 11.84NKK92 pKa = 9.36CKK94 pKa = 10.25GYY96 pKa = 9.69RR97 pKa = 11.84ASFCRR102 pKa = 11.84PDD104 pKa = 3.62LQFDD108 pKa = 4.38SNGKK112 pKa = 9.38ALTMRR117 pKa = 11.84CQKK120 pKa = 10.46RR121 pKa = 11.84EE122 pKa = 3.65MMRR125 pKa = 11.84VVRR128 pKa = 5.11

Molecular weight: 15.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

16090

44

2206

206.3

23.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.155 ± 0.63

1.231 ± 0.185

6.24 ± 0.219

7.073 ± 0.344

4.046 ± 0.291

7.589 ± 0.396

1.753 ± 0.255

6.01 ± 0.183

6.917 ± 0.291

6.737 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.921 ± 0.199

4.86 ± 0.323

3.108 ± 0.234

3.86 ± 0.266

5.245 ± 0.269

5.848 ± 0.213

5.6 ± 0.323

6.998 ± 0.214

1.448 ± 0.124

3.362 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski