Bifidobacterium phage BlindUri1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XZC8|A0A6G6XZC8_9CAUD Uncharacterized protein OS=Bifidobacterium phage BlindUri1 OX=2713249 GN=BRUR0010001c01_00023 PE=4 SV=1
MM1 pKa = 7.36ATFIEE6 pKa = 4.58DD7 pKa = 3.55TEE9 pKa = 4.28KK10 pKa = 10.81AIKK13 pKa = 10.46NSGHH17 pKa = 6.43APSDD21 pKa = 3.2IEE23 pKa = 5.13FIGDD27 pKa = 3.41YY28 pKa = 9.48TRR30 pKa = 11.84HH31 pKa = 5.63VDD33 pKa = 3.7LGYY36 pKa = 10.11WDD38 pKa = 4.14TFRR41 pKa = 11.84KK42 pKa = 9.95AVTHH46 pKa = 6.01YY47 pKa = 10.39EE48 pKa = 3.93EE49 pKa = 5.16SDD51 pKa = 3.27NGSQDD56 pKa = 3.04SGYY59 pKa = 10.27KK60 pKa = 9.75ACRR63 pKa = 11.84DD64 pKa = 3.96LIIAFQDD71 pKa = 3.31GSLLFRR77 pKa = 11.84EE78 pKa = 6.27GYY80 pKa = 8.29ATTEE84 pKa = 3.6QWDD87 pKa = 4.09YY88 pKa = 11.67LPSSNSAAYY97 pKa = 10.21DD98 pKa = 3.68PDD100 pKa = 3.79YY101 pKa = 11.01LPPEE105 pKa = 4.28SAIQSTFEE113 pKa = 4.34SGFSLIQKK121 pKa = 9.45AINNRR126 pKa = 11.84SEE128 pKa = 4.46SYY130 pKa = 10.46IEE132 pKa = 3.84QLKK135 pKa = 10.75SDD137 pKa = 4.77GVSDD141 pKa = 4.07WNIQQQ146 pKa = 3.3

Molecular weight: 16.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XZH7|A0A6G6XZH7_9CAUD Uncharacterized protein OS=Bifidobacterium phage BlindUri1 OX=2713249 GN=BRUR0010001c01_00028 PE=4 SV=1
MM1 pKa = 7.5TYY3 pKa = 8.9YY4 pKa = 10.47TFRR7 pKa = 11.84FDD9 pKa = 4.09SGWDD13 pKa = 2.92KK14 pKa = 10.52RR15 pKa = 11.84TRR17 pKa = 11.84QNLNAGLVRR26 pKa = 11.84MVLDD30 pKa = 3.32IHH32 pKa = 7.14NMAVHH37 pKa = 6.87RR38 pKa = 11.84APVKK42 pKa = 9.92TGALINSGRR51 pKa = 11.84YY52 pKa = 8.77NRR54 pKa = 11.84TGPLTYY60 pKa = 10.49QLTFGNSRR68 pKa = 11.84VPYY71 pKa = 9.71AVIRR75 pKa = 11.84EE76 pKa = 4.15EE77 pKa = 4.26RR78 pKa = 11.84NRR80 pKa = 11.84LHH82 pKa = 7.04PWTTHH87 pKa = 4.56YY88 pKa = 10.8LRR90 pKa = 11.84NSVNEE95 pKa = 3.72ASGRR99 pKa = 11.84ASQYY103 pKa = 10.57FKK105 pKa = 11.47EE106 pKa = 4.33LMM108 pKa = 4.29

Molecular weight: 12.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

9450

58

736

205.4

22.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.778 ± 0.449

0.942 ± 0.126

6.72 ± 0.397

4.772 ± 0.384

2.952 ± 0.184

6.698 ± 0.476

1.714 ± 0.155

6.233 ± 0.288

5.026 ± 0.343

7.852 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.172

4.825 ± 0.251

5.164 ± 0.247

4.698 ± 0.381

4.794 ± 0.301

6.889 ± 0.322

7.577 ± 0.284

7.005 ± 0.38

1.915 ± 0.194

4.074 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski