Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae; Chloroflexaceae; Chloroflexus; Chloroflexus aurantiacus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3850 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9WFB5|A9WFB5_CHLAA NADH-quinone oxidoreductase E subunit OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) OX=324602 GN=Caur_2900 PE=3 SV=1
MM1 pKa = 7.14TVVPLPRR8 pKa = 11.84PAFPLPLPRR17 pKa = 11.84SPITLLPLSLLLVLLLAAIQPLVVFIAPVLPSTTTPALPYY57 pKa = 10.71ARR59 pKa = 11.84TDD61 pKa = 3.34SNVFIEE67 pKa = 4.15NWGQFDD73 pKa = 3.84PAFVAILIGQDD84 pKa = 2.56ARR86 pKa = 11.84LAVGHH91 pKa = 6.98DD92 pKa = 3.81GRR94 pKa = 11.84LHH96 pKa = 5.68LHH98 pKa = 6.21VDD100 pKa = 4.14GAAPLTFTAAGVTTTPDD117 pKa = 3.17VSLHH121 pKa = 6.22GPLATTISFLTGNDD135 pKa = 3.49PNQHH139 pKa = 5.04VNVPVWEE146 pKa = 4.37VARR149 pKa = 11.84LALTPGVTLEE159 pKa = 4.13LTVIDD164 pKa = 4.53GRR166 pKa = 11.84LRR168 pKa = 11.84LCARR172 pKa = 11.84TADD175 pKa = 3.68PTLLNRR181 pKa = 11.84VQMQVSGGEE190 pKa = 4.13VIGMVDD196 pKa = 4.38GEE198 pKa = 4.9LEE200 pKa = 4.38VQAGAHH206 pKa = 6.26RR207 pKa = 11.84LRR209 pKa = 11.84LPLLEE214 pKa = 4.64AVTPAGIPHH223 pKa = 6.02QQTFSPAIAPDD234 pKa = 3.84GVVRR238 pKa = 11.84APVGIPEE245 pKa = 5.22GIGITGQATPTQTSAAMLLGASTFVGGAAGSGEE278 pKa = 4.22DD279 pKa = 3.46TATAMVIDD287 pKa = 4.37GQGAVYY293 pKa = 10.44VAGNTGSADD302 pKa = 3.42FPTTLGAYY310 pKa = 9.19DD311 pKa = 3.66RR312 pKa = 11.84SLNGGDD318 pKa = 3.57RR319 pKa = 11.84GDD321 pKa = 3.91AFVSRR326 pKa = 11.84LSGDD330 pKa = 3.48LRR332 pKa = 11.84NLLAGTFLGGSDD344 pKa = 3.02WDD346 pKa = 4.01EE347 pKa = 4.77ANDD350 pKa = 4.7LALDD354 pKa = 3.86AQGNVYY360 pKa = 10.26VAGEE364 pKa = 4.13TSSTDD369 pKa = 3.49FPTTLGAYY377 pKa = 9.58DD378 pKa = 3.37RR379 pKa = 11.84TYY381 pKa = 11.33NGDD384 pKa = 3.36GSDD387 pKa = 3.56VFVSRR392 pKa = 11.84LSGDD396 pKa = 3.46LGSLLASTFLGGSYY410 pKa = 9.79WNFAGALALDD420 pKa = 4.33GQGTVYY426 pKa = 10.72VAGGTSSTDD435 pKa = 3.44FPTTLGAYY443 pKa = 9.61DD444 pKa = 3.32RR445 pKa = 11.84TYY447 pKa = 11.38NGGSSDD453 pKa = 3.72VFVSRR458 pKa = 11.84LSGDD462 pKa = 3.85LNSLLASTFLGGSAWDD478 pKa = 3.47SATALVLDD486 pKa = 4.34GQGNVYY492 pKa = 10.25VAGEE496 pKa = 4.19TGSADD501 pKa = 3.79FPTMAGAYY509 pKa = 9.45DD510 pKa = 3.88GSYY513 pKa = 11.25NSGDD517 pKa = 3.28RR518 pKa = 11.84DD519 pKa = 3.54AFASKK524 pKa = 10.77LSGDD528 pKa = 4.02LGSLLTSTFLGGSDD542 pKa = 3.09WDD544 pKa = 3.88VATALALDD552 pKa = 4.21GQGTVYY558 pKa = 10.59VAGYY562 pKa = 8.22TSSTNFPTTLGAYY575 pKa = 9.61DD576 pKa = 3.32RR577 pKa = 11.84TYY579 pKa = 11.57NGGSSDD585 pKa = 3.42AFAGRR590 pKa = 11.84LSGDD594 pKa = 3.48LGSLLTSTFLGGNDD608 pKa = 3.2SEE610 pKa = 4.83YY611 pKa = 11.38ALSLALDD618 pKa = 4.02GQGAVYY624 pKa = 11.08VMGEE628 pKa = 4.07TGSTIFPTTAGAYY641 pKa = 9.85DD642 pKa = 3.3RR643 pKa = 11.84VYY645 pKa = 11.18NGGYY649 pKa = 9.39RR650 pKa = 11.84DD651 pKa = 3.74AFVSRR656 pKa = 11.84LSSNLGSLLASTFLGGSGWDD676 pKa = 3.29VAIALTLDD684 pKa = 3.72GQGTVYY690 pKa = 10.59VAGYY694 pKa = 8.22TSSTNFPTTLGAYY707 pKa = 9.64DD708 pKa = 3.91GSYY711 pKa = 10.99NGGDD715 pKa = 3.13RR716 pKa = 11.84DD717 pKa = 3.81AFASKK722 pKa = 10.77LSGDD726 pKa = 4.02LGSLLTSTFLGGMFGNGNDD745 pKa = 3.65VANSLAISSQDD756 pKa = 3.28TVYY759 pKa = 10.77VAGWTGSANFPTTAGAYY776 pKa = 10.06DD777 pKa = 3.68GVFNRR782 pKa = 11.84GFRR785 pKa = 11.84DD786 pKa = 3.41AFVSRR791 pKa = 11.84LSSDD795 pKa = 3.31LGSLLASTFLGGSDD809 pKa = 3.09WDD811 pKa = 3.78VATALVLDD819 pKa = 4.39GQGNVYY825 pKa = 10.24VAGEE829 pKa = 4.06TWSANFPTTAGAYY842 pKa = 9.48DD843 pKa = 3.34RR844 pKa = 11.84TYY846 pKa = 11.51NGGARR851 pKa = 11.84DD852 pKa = 3.71AFVSKK857 pKa = 10.88LSGNLGSLLASTFLGGSDD875 pKa = 3.36WDD877 pKa = 3.91SATALALDD885 pKa = 4.06GQGNVYY891 pKa = 10.31VAGDD895 pKa = 3.77TYY897 pKa = 11.17STDD900 pKa = 3.7FPTTAGVYY908 pKa = 10.03DD909 pKa = 3.69RR910 pKa = 11.84AYY912 pKa = 11.08NGGGSDD918 pKa = 3.54TFVSRR923 pKa = 11.84LSGDD927 pKa = 3.2LGGLLAGSFLGGSLPDD943 pKa = 3.45SATALALDD951 pKa = 4.06GQGNVYY957 pKa = 9.68VAGWTDD963 pKa = 3.32SANFPTTAGSYY974 pKa = 10.34DD975 pKa = 3.28GSFNGSDD982 pKa = 3.46GFVSKK987 pKa = 10.95LSGEE991 pKa = 4.1LRR993 pKa = 11.84SLVASTFLGGSIDD1006 pKa = 3.85EE1007 pKa = 4.6SATALTLDD1015 pKa = 3.75GQGNVYY1021 pKa = 10.25VAGEE1025 pKa = 4.13TSSTDD1030 pKa = 3.49FPTTLGAHH1038 pKa = 6.45DD1039 pKa = 3.52RR1040 pKa = 11.84TYY1042 pKa = 11.44NGGGSDD1048 pKa = 3.64VFVSRR1053 pKa = 11.84LSGDD1057 pKa = 3.55LGSQLASTFLGGSDD1071 pKa = 3.36WDD1073 pKa = 3.82SATALVLDD1081 pKa = 4.58GQGSVYY1087 pKa = 10.62VAGEE1091 pKa = 4.05TGSADD1096 pKa = 3.79FPTMAGAYY1104 pKa = 9.12DD1105 pKa = 3.95GSYY1108 pKa = 10.44NGSGDD1113 pKa = 3.7TFVSRR1118 pKa = 11.84LSGNLGGLLASTFLGGSAWDD1138 pKa = 3.57SATALALDD1146 pKa = 4.19GQGTVYY1152 pKa = 10.56VAGYY1156 pKa = 8.05TEE1158 pKa = 5.01SNNFPITAGAYY1169 pKa = 8.54GGVYY1173 pKa = 10.53NGDD1176 pKa = 2.95RR1177 pKa = 11.84DD1178 pKa = 3.98AFVSKK1183 pKa = 10.67LVFAFTKK1190 pKa = 9.91TSSAVSTTNQSINLTLQWQSAGATVHH1216 pKa = 6.78HH1217 pKa = 6.36YY1218 pKa = 10.13RR1219 pKa = 11.84YY1220 pKa = 10.48CLDD1223 pKa = 3.84TNPGCTPTTNVGANTSVTVAGITPNTTYY1251 pKa = 10.07YY1252 pKa = 8.51WQVRR1256 pKa = 11.84ACADD1260 pKa = 3.21SDD1262 pKa = 4.09CAVFIDD1268 pKa = 5.13ANNGQHH1274 pKa = 6.36WSLKK1278 pKa = 7.49TSFMIYY1284 pKa = 9.1LTFAARR1290 pKa = 3.46

Molecular weight: 132.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9WGV5|A9WGV5_CHLAA Glycosyl hydrolase 53 domain protein OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) OX=324602 GN=Caur_3072 PE=4 SV=1
MM1 pKa = 7.56AANPKK6 pKa = 8.75TATPRR11 pKa = 11.84PRR13 pKa = 11.84HH14 pKa = 4.85VPKK17 pKa = 10.64RR18 pKa = 11.84MCIVCRR24 pKa = 11.84QSDD27 pKa = 3.65AKK29 pKa = 10.43RR30 pKa = 11.84ALIRR34 pKa = 11.84LVRR37 pKa = 11.84NADD40 pKa = 3.08GRR42 pKa = 11.84VVIDD46 pKa = 3.85PTGKK50 pKa = 9.46QPGRR54 pKa = 11.84GAYY57 pKa = 9.77LCRR60 pKa = 11.84NPLCWAQALRR70 pKa = 11.84RR71 pKa = 11.84NALEE75 pKa = 4.06RR76 pKa = 11.84ALKK79 pKa = 10.21IATLHH84 pKa = 6.39PDD86 pKa = 3.05DD87 pKa = 4.58RR88 pKa = 11.84AVIEE92 pKa = 5.09AMGQQLLQEE101 pKa = 4.49TEE103 pKa = 4.29STASAA108 pKa = 3.69

Molecular weight: 11.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3850

0

3850

1405705

36

5505

365.1

40.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.275 ± 0.054

0.818 ± 0.011

5.119 ± 0.035

5.401 ± 0.039

3.4 ± 0.025

7.633 ± 0.042

2.124 ± 0.021

5.943 ± 0.032

1.781 ± 0.026

11.305 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.006 ± 0.015

2.732 ± 0.032

5.909 ± 0.033

4.226 ± 0.028

7.585 ± 0.041

4.928 ± 0.033

6.009 ± 0.052

7.499 ± 0.03

1.595 ± 0.019

2.712 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski