Clostridium sp. CAG:306

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6P7D6|R6P7D6_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:306 OX=1262794 GN=BN597_01902 PE=4 SV=1
MM1 pKa = 7.35NLNEE5 pKa = 4.18EE6 pKa = 4.52CLLKK10 pKa = 10.83YY11 pKa = 10.0LQNPDD16 pKa = 3.67EE17 pKa = 4.96LVDD20 pKa = 3.94LTSLLLEE27 pKa = 4.45FNNDD31 pKa = 3.19YY32 pKa = 11.06EE33 pKa = 4.77KK34 pKa = 11.49NN35 pKa = 3.59

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6PBQ8|R6PBQ8_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:306 OX=1262794 GN=BN597_00526 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84RR3 pKa = 11.84TLEE6 pKa = 3.61GTKK9 pKa = 9.97RR10 pKa = 11.84KK11 pKa = 8.92RR12 pKa = 11.84QNVSGFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84TVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84HH40 pKa = 5.15RR41 pKa = 11.84LAIQAKK47 pKa = 9.31KK48 pKa = 10.18RR49 pKa = 11.84AA50 pKa = 3.68

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2130

0

2130

626252

29

2307

294.0

33.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.202 ± 0.054

1.272 ± 0.027

5.769 ± 0.051

6.783 ± 0.058

4.509 ± 0.046

5.767 ± 0.072

1.523 ± 0.021

8.146 ± 0.054

9.097 ± 0.068

8.844 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.03

6.187 ± 0.062

3.321 ± 0.034

3.534 ± 0.033

3.276 ± 0.037

6.063 ± 0.048

5.392 ± 0.045

6.157 ± 0.047

0.678 ± 0.016

3.946 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski