Hubei tombus-like virus 29

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGC0|A0A1L3KGC0_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 29 OX=1923276 PE=4 SV=1
MM1 pKa = 8.15RR2 pKa = 11.84PRR4 pKa = 11.84LVMNRR9 pKa = 11.84SVSDD13 pKa = 3.52PAFVSWVIGDD23 pKa = 4.04PLLSNRR29 pKa = 11.84EE30 pKa = 3.75ARR32 pKa = 11.84NGPKK36 pKa = 9.73RR37 pKa = 11.84HH38 pKa = 6.29SDD40 pKa = 3.08SSIGPTRR47 pKa = 11.84QPAYY51 pKa = 9.65VTTLHH56 pKa = 6.19NVAAEE61 pKa = 4.29TFGKK65 pKa = 9.44LLSMGYY71 pKa = 9.91DD72 pKa = 3.4VQWNYY77 pKa = 10.32ICNLILQHH85 pKa = 6.79GIGSTEE91 pKa = 4.07RR92 pKa = 11.84IIDD95 pKa = 3.39IARR98 pKa = 11.84NEE100 pKa = 4.14CSMIPKK106 pKa = 10.32FSDD109 pKa = 3.16NEE111 pKa = 4.12GRR113 pKa = 11.84QHH115 pKa = 7.49LEE117 pKa = 3.87AVDD120 pKa = 3.55KK121 pKa = 10.77HH122 pKa = 6.37DD123 pKa = 6.18KK124 pKa = 10.48IVADD128 pKa = 3.79TKK130 pKa = 10.69LVKK133 pKa = 10.7AEE135 pKa = 3.71ARR137 pKa = 11.84KK138 pKa = 9.81IEE140 pKa = 4.07LEE142 pKa = 3.92NVKK145 pKa = 10.54LEE147 pKa = 4.17KK148 pKa = 10.96EE149 pKa = 4.1NVLLEE154 pKa = 4.29KK155 pKa = 10.91GLDD158 pKa = 3.38IKK160 pKa = 11.03KK161 pKa = 10.39LEE163 pKa = 4.05QTIQQLGMEE172 pKa = 4.59KK173 pKa = 10.07EE174 pKa = 3.99HH175 pKa = 7.69AILLKK180 pKa = 10.6NDD182 pKa = 2.93IDD184 pKa = 3.69ILDD187 pKa = 3.97AYY189 pKa = 10.53GYY191 pKa = 10.91LCAQTIGQQITNKK204 pKa = 10.25HH205 pKa = 4.83EE206 pKa = 4.24TTCANICRR214 pKa = 11.84NYY216 pKa = 9.61FAKK219 pKa = 10.63SQITDD224 pKa = 2.61NMMILHH230 pKa = 8.15AIRR233 pKa = 11.84IILAMYY239 pKa = 10.26SVDD242 pKa = 3.7RR243 pKa = 11.84KK244 pKa = 10.2FAMTSYY250 pKa = 10.84FDD252 pKa = 3.52WDD254 pKa = 3.62AKK256 pKa = 11.11GKK258 pKa = 10.46DD259 pKa = 3.64DD260 pKa = 5.09FEE262 pKa = 4.84KK263 pKa = 10.7LRR265 pKa = 11.84KK266 pKa = 9.36KK267 pKa = 10.79ADD269 pKa = 3.55VEE271 pKa = 4.25HH272 pKa = 6.87KK273 pKa = 8.46PWKK276 pKa = 8.79VIKK279 pKa = 10.69GITAVNKK286 pKa = 8.54MRR288 pKa = 11.84AGITTRR294 pKa = 11.84SRR296 pKa = 11.84FFGLFNKK303 pKa = 10.13KK304 pKa = 10.37YY305 pKa = 9.72EE306 pKa = 4.19LTSFF310 pKa = 3.95

Molecular weight: 35.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGC0|A0A1L3KGC0_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 29 OX=1923276 PE=4 SV=1
MM1 pKa = 6.78NTQLFKK7 pKa = 10.85YY8 pKa = 9.41IKK10 pKa = 9.21KK11 pKa = 9.71FVVKK15 pKa = 10.03VEE17 pKa = 3.64PWTFDD22 pKa = 2.97EE23 pKa = 5.49YY24 pKa = 10.55IASMSSASKK33 pKa = 9.85RR34 pKa = 11.84KK35 pKa = 9.55QYY37 pKa = 11.17SDD39 pKa = 4.97YY40 pKa = 10.82YY41 pKa = 10.97DD42 pKa = 3.07QYY44 pKa = 11.14LRR46 pKa = 11.84NGRR49 pKa = 11.84IPSYY53 pKa = 9.17ITPFTKK59 pKa = 9.42IEE61 pKa = 4.01KK62 pKa = 9.28MSSGKK67 pKa = 10.38YY68 pKa = 8.78KK69 pKa = 10.37APRR72 pKa = 11.84MIQGRR77 pKa = 11.84HH78 pKa = 3.96MVFNLHH84 pKa = 5.66YY85 pKa = 10.28GRR87 pKa = 11.84YY88 pKa = 8.72IKK90 pKa = 10.31PLEE93 pKa = 4.3KK94 pKa = 10.42EE95 pKa = 3.95VTKK98 pKa = 10.79YY99 pKa = 11.08GKK101 pKa = 10.42FSIHH105 pKa = 6.35FGKK108 pKa = 10.89GNYY111 pKa = 7.84NQQAEE116 pKa = 4.8KK117 pKa = 10.42IFKK120 pKa = 10.37LSQKK124 pKa = 9.18YY125 pKa = 9.66KK126 pKa = 10.38YY127 pKa = 9.11YY128 pKa = 10.08TEE130 pKa = 4.94CDD132 pKa = 3.17HH133 pKa = 6.94TSFDD137 pKa = 3.14AHH139 pKa = 5.04VTVEE143 pKa = 4.03QLRR146 pKa = 11.84LTHH149 pKa = 6.96RR150 pKa = 11.84FYY152 pKa = 11.49NSCFPGYY159 pKa = 10.5ASDD162 pKa = 3.78VARR165 pKa = 11.84LAKK168 pKa = 9.5RR169 pKa = 11.84TINNTCISRR178 pKa = 11.84TGDD181 pKa = 2.77IYY183 pKa = 10.73RR184 pKa = 11.84VRR186 pKa = 11.84GSRR189 pKa = 11.84MSGDD193 pKa = 3.15VDD195 pKa = 3.54TGFGNCLINYY205 pKa = 8.01AILKK209 pKa = 8.09AACSNLHH216 pKa = 6.23IKK218 pKa = 10.79CEE220 pKa = 4.17IIVNGDD226 pKa = 3.26DD227 pKa = 4.55SIIFTDD233 pKa = 3.91VPIPTAEE240 pKa = 3.95LSAEE244 pKa = 3.73LRR246 pKa = 11.84KK247 pKa = 10.53YY248 pKa = 11.2NMEE251 pKa = 4.3SKK253 pKa = 10.72VNPSVSDD260 pKa = 2.93IHH262 pKa = 8.17KK263 pKa = 10.75VEE265 pKa = 4.73FCRR268 pKa = 11.84TKK270 pKa = 10.93LVFNASAKK278 pKa = 7.2PTMMIDD284 pKa = 3.68PKK286 pKa = 10.77RR287 pKa = 11.84LIDD290 pKa = 3.89IYY292 pKa = 10.83GMCYY296 pKa = 10.0TISKK300 pKa = 9.92KK301 pKa = 10.43NYY303 pKa = 7.91HH304 pKa = 6.0QYY306 pKa = 11.27LLEE309 pKa = 4.01TAMCNSYY316 pKa = 10.77INCNNPLGVLWAKK329 pKa = 9.96YY330 pKa = 10.11FNIDD334 pKa = 4.39LISYY338 pKa = 8.91KK339 pKa = 10.57KK340 pKa = 10.36NEE342 pKa = 3.77KK343 pKa = 10.54SKK345 pKa = 10.97IKK347 pKa = 10.0ILQNIKK353 pKa = 10.56CLEE356 pKa = 3.84KK357 pKa = 11.06DD358 pKa = 3.93KK359 pKa = 10.64ILKK362 pKa = 9.31MMSLSPDD369 pKa = 3.02EE370 pKa = 4.42TTTEE374 pKa = 3.97EE375 pKa = 4.28VTPSMLAAWPDD386 pKa = 3.14IYY388 pKa = 11.11SIEE391 pKa = 4.03AQIKK395 pKa = 8.96KK396 pKa = 10.25LADD399 pKa = 3.09RR400 pKa = 11.84VLNRR404 pKa = 11.84KK405 pKa = 7.67YY406 pKa = 10.23QYY408 pKa = 11.41KK409 pKa = 9.89LVEE412 pKa = 4.19RR413 pKa = 11.84DD414 pKa = 3.1FHH416 pKa = 7.5INHH419 pKa = 7.64DD420 pKa = 3.27IQFIVKK426 pKa = 8.81YY427 pKa = 9.13QAA429 pKa = 3.33

Molecular weight: 50.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

739

310

429

369.5

42.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.954 ± 0.641

2.165 ± 0.31

5.683 ± 0.431

5.413 ± 0.402

3.924 ± 0.211

4.33 ± 0.466

2.706 ± 0.11

8.796 ± 0.048

10.014 ± 0.37

7.307 ± 0.606

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.248 ± 0.012

5.683 ± 0.112

3.112 ± 0.298

3.383 ± 0.093

5.142 ± 0.373

6.36 ± 0.673

5.413 ± 0.04

5.007 ± 0.087

0.947 ± 0.193

5.413 ± 1.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski