Hydrogenispora ethanolica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Firmicutes sensu stricto incertae sedis; Hydrogenispora

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5259 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1RWC6|A0A4R1RWC6_9FIRM FlgN protein OS=Hydrogenispora ethanolica OX=1082276 GN=EDC14_1008138 PE=4 SV=1
MM1 pKa = 8.18RR2 pKa = 11.84EE3 pKa = 4.0LRR5 pKa = 11.84EE6 pKa = 3.52QAAYY10 pKa = 10.64LRR12 pKa = 11.84GLMEE16 pKa = 4.39GSEE19 pKa = 4.14LAKK22 pKa = 10.43DD23 pKa = 4.21DD24 pKa = 3.73KK25 pKa = 11.62SKK27 pKa = 11.17LIWEE31 pKa = 4.54GLMDD35 pKa = 4.5FCDD38 pKa = 4.97GVAEE42 pKa = 4.72DD43 pKa = 3.95LAEE46 pKa = 4.16MEE48 pKa = 4.33EE49 pKa = 4.39SQEE52 pKa = 3.83EE53 pKa = 3.94FADD56 pKa = 3.97YY57 pKa = 10.88IEE59 pKa = 6.22AIDD62 pKa = 4.35EE63 pKa = 4.39DD64 pKa = 4.89LSTLEE69 pKa = 4.29KK70 pKa = 10.81YY71 pKa = 10.47FYY73 pKa = 10.5DD74 pKa = 4.13AEE76 pKa = 4.34GSEE79 pKa = 4.29DD80 pKa = 3.78SEE82 pKa = 4.67EE83 pKa = 4.41LVTSQPQEE91 pKa = 4.3DD92 pKa = 4.1DD93 pKa = 3.86DD94 pKa = 4.59EE95 pKa = 6.55EE96 pKa = 5.19IMEE99 pKa = 4.69LTCPHH104 pKa = 7.14CKK106 pKa = 9.31QDD108 pKa = 5.33LYY110 pKa = 11.56FEE112 pKa = 5.25DD113 pKa = 4.51EE114 pKa = 4.24PGNYY118 pKa = 9.25EE119 pKa = 4.9VVCPDD124 pKa = 3.62CGNVVWQHH132 pKa = 4.98FTAEE136 pKa = 4.42LSPEE140 pKa = 4.39GEE142 pKa = 4.27HH143 pKa = 7.16PLAA146 pKa = 5.69

Molecular weight: 16.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1SBF1|A0A4R1SBF1_9FIRM Uncharacterized protein OS=Hydrogenispora ethanolica OX=1082276 GN=EDC14_1001158 PE=4 SV=1
MM1 pKa = 6.97HH2 pKa = 7.14HH3 pKa = 6.46SVRR6 pKa = 11.84PGAQPFRR13 pKa = 11.84DD14 pKa = 3.37ARR16 pKa = 11.84PRR18 pKa = 11.84VVPRR22 pKa = 11.84RR23 pKa = 11.84QFRR26 pKa = 11.84PAAAQWGAPSGTNRR40 pKa = 11.84APGRR44 pKa = 11.84TRR46 pKa = 11.84VRR48 pKa = 11.84TARR51 pKa = 11.84RR52 pKa = 11.84IRR54 pKa = 11.84KK55 pKa = 7.06TGRR58 pKa = 11.84KK59 pKa = 8.91PFVQGQTNRR68 pKa = 11.84KK69 pKa = 9.01ALEE72 pKa = 4.3HH73 pKa = 4.54VWKK76 pKa = 10.63RR77 pKa = 11.84LTHH80 pKa = 6.54DD81 pKa = 4.03FSIALL86 pKa = 3.81

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5259

0

5259

1646465

25

3595

313.1

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.396 ± 0.044

1.063 ± 0.012

4.715 ± 0.026

6.381 ± 0.039

4.285 ± 0.022

7.797 ± 0.031

1.802 ± 0.013

6.649 ± 0.03

4.889 ± 0.037

10.88 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.017

3.646 ± 0.027

4.523 ± 0.025

3.903 ± 0.023

6.014 ± 0.035

5.317 ± 0.026

4.922 ± 0.03

6.908 ± 0.03

1.245 ± 0.014

3.274 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski