Mycobacterium phage GaugeLDP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D965|A0A5P8D965_9CAUD Immunity repressor OS=Mycobacterium phage GaugeLDP OX=2652419 GN=71 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.15SYY4 pKa = 10.83FITISIAFVTAITVLTFADD23 pKa = 4.66DD24 pKa = 3.58EE25 pKa = 4.77ASANVPFTQAAFPCEE40 pKa = 3.95EE41 pKa = 4.7DD42 pKa = 3.63EE43 pKa = 4.58VLGYY47 pKa = 10.86APQFGPNNVGCIHH60 pKa = 6.83VDD62 pKa = 4.37LIKK65 pKa = 10.72RR66 pKa = 3.64

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D9X6|A0A5P8D9X6_9CAUD Uncharacterized protein OS=Mycobacterium phage GaugeLDP OX=2652419 GN=48 PE=4 SV=1
MM1 pKa = 7.9PPRR4 pKa = 11.84ASIQQTAEE12 pKa = 3.81YY13 pKa = 10.28LGVHH17 pKa = 6.02VNTVRR22 pKa = 11.84NYY24 pKa = 9.35IAEE27 pKa = 4.6GKK29 pKa = 10.0LKK31 pKa = 10.4AVRR34 pKa = 11.84LGPRR38 pKa = 11.84LIRR41 pKa = 11.84VEE43 pKa = 4.05RR44 pKa = 11.84DD45 pKa = 3.15SVEE48 pKa = 3.78ALMRR52 pKa = 11.84PIGKK56 pKa = 9.51

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

16525

33

839

183.6

20.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.033 ± 0.378

0.853 ± 0.102

6.142 ± 0.224

6.796 ± 0.31

3.377 ± 0.183

8.424 ± 0.454

1.961 ± 0.147

5.005 ± 0.171

4.623 ± 0.227

8.182 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.141

3.201 ± 0.193

5.731 ± 0.285

3.631 ± 0.191

6.608 ± 0.392

5.216 ± 0.224

5.888 ± 0.235

6.959 ± 0.217

1.9 ± 0.154

2.874 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski