Clostridium sp. HMP27

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3451 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A099S0W7|A0A099S0W7_9CLOT Regulator OS=Clostridium sp. HMP27 OX=1487921 GN=DP68_13785 PE=3 SV=1
MM1 pKa = 7.98DD2 pKa = 5.62SIKK5 pKa = 11.02SRR7 pKa = 11.84VSYY10 pKa = 10.89LKK12 pKa = 11.14GLMDD16 pKa = 4.8GLKK19 pKa = 9.68IDD21 pKa = 4.93ANDD24 pKa = 3.55NEE26 pKa = 4.51GKK28 pKa = 10.25VIRR31 pKa = 11.84EE32 pKa = 3.77IVDD35 pKa = 3.45VLEE38 pKa = 5.11EE39 pKa = 4.04IADD42 pKa = 3.91EE43 pKa = 4.34LDD45 pKa = 3.44EE46 pKa = 5.88LKK48 pKa = 10.84DD49 pKa = 3.56SQKK52 pKa = 11.61DD53 pKa = 3.34MEE55 pKa = 4.71EE56 pKa = 4.21YY57 pKa = 11.01VDD59 pKa = 5.29NLDD62 pKa = 3.67EE63 pKa = 4.21NLAYY67 pKa = 9.65IEE69 pKa = 5.64DD70 pKa = 4.1EE71 pKa = 4.63LFDD74 pKa = 6.45DD75 pKa = 5.4EE76 pKa = 7.13DD77 pKa = 5.25DD78 pKa = 5.78DD79 pKa = 5.64YY80 pKa = 12.08DD81 pKa = 4.7EE82 pKa = 5.06EE83 pKa = 7.24DD84 pKa = 2.83IDD86 pKa = 4.48GFVEE90 pKa = 4.96VACPDD95 pKa = 3.64CGEE98 pKa = 4.26TVYY101 pKa = 10.47IDD103 pKa = 4.71SGILDD108 pKa = 3.88EE109 pKa = 5.54KK110 pKa = 11.14EE111 pKa = 5.08SIICPNCHH119 pKa = 6.78KK120 pKa = 10.61DD121 pKa = 2.99IPLQFEE127 pKa = 4.58CDD129 pKa = 3.71GHH131 pKa = 8.12CGEE134 pKa = 4.85CTEE137 pKa = 5.08DD138 pKa = 3.18

Molecular weight: 15.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A099S2U3|A0A099S2U3_9CLOT Uncharacterized protein OS=Clostridium sp. HMP27 OX=1487921 GN=DP68_15245 PE=4 SV=1
MM1 pKa = 7.26SRR3 pKa = 11.84EE4 pKa = 4.01SGRR7 pKa = 11.84DD8 pKa = 3.01RR9 pKa = 11.84DD10 pKa = 3.56RR11 pKa = 11.84DD12 pKa = 3.29RR13 pKa = 11.84DD14 pKa = 3.56NGNRR18 pKa = 11.84RR19 pKa = 11.84SSGKK23 pKa = 7.82MRR25 pKa = 11.84RR26 pKa = 11.84SRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 8.5VCMFCVDD37 pKa = 3.25KK38 pKa = 11.49AEE40 pKa = 4.61SIDD43 pKa = 3.62YY44 pKa = 10.94KK45 pKa = 11.04DD46 pKa = 3.2INKK49 pKa = 9.01LRR51 pKa = 11.84KK52 pKa = 9.78YY53 pKa = 8.01ITEE56 pKa = 4.01RR57 pKa = 11.84GKK59 pKa = 10.04ILPRR63 pKa = 11.84RR64 pKa = 11.84ISGTCAKK71 pKa = 9.65HH72 pKa = 5.82QRR74 pKa = 11.84ALTDD78 pKa = 4.44AIKK81 pKa = 10.41RR82 pKa = 11.84ARR84 pKa = 11.84NIALLPFTTEE94 pKa = 3.59

Molecular weight: 11.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3451

0

3451

1021203

33

1449

295.9

33.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.035 ± 0.044

1.189 ± 0.016

5.227 ± 0.03

7.482 ± 0.047

4.351 ± 0.029

6.673 ± 0.041

1.421 ± 0.016

9.871 ± 0.049

8.714 ± 0.039

9.118 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.786 ± 0.018

5.864 ± 0.033

2.944 ± 0.023

2.413 ± 0.017

3.424 ± 0.03

6.292 ± 0.034

4.913 ± 0.026

6.64 ± 0.034

0.688 ± 0.014

3.957 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski