Synechococcus phage ACG-2014i

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3FGE6|A0A0E3FGE6_9CAUD Peptidase_S8 domain-containing protein OS=Synechococcus phage ACG-2014i OX=1493513 GN=Syn7803US120_170 PE=4 SV=1
MM1 pKa = 7.43SKK3 pKa = 10.01ILANQIANYY12 pKa = 9.63GDD14 pKa = 3.53NSPVEE19 pKa = 4.3VKK21 pKa = 10.66EE22 pKa = 4.11GVNIPAGKK30 pKa = 8.71PLQAAGVAGTSGQVLTATGASIQWTTPFDD59 pKa = 3.68GSYY62 pKa = 9.46LTLTNKK68 pKa = 8.43PTIPAAQINADD79 pKa = 3.45WNASGGSVAAILNKK93 pKa = 9.8PVIPAQPSIVLTAAGSSTLTYY114 pKa = 10.25NQANGEE120 pKa = 4.31FTFTPPDD127 pKa = 3.67FSSYY131 pKa = 9.15LTTYY135 pKa = 10.41TEE137 pKa = 3.91TDD139 pKa = 4.26PIFLASPAYY148 pKa = 10.29QITNQNRR155 pKa = 11.84TDD157 pKa = 3.07WGLAYY162 pKa = 10.44SWGDD166 pKa = 3.3HH167 pKa = 5.43SQAGYY172 pKa = 7.87ITTYY176 pKa = 10.68TEE178 pKa = 3.7TDD180 pKa = 3.48PVFTASVAAGITTQNKK196 pKa = 7.69TNWDD200 pKa = 3.34TSYY203 pKa = 11.26GWGNHH208 pKa = 5.07AAQGYY213 pKa = 5.2ITSYY217 pKa = 11.24SEE219 pKa = 3.72TDD221 pKa = 3.46PVFTASVAAGITLTNVSNWDD241 pKa = 3.33TAYY244 pKa = 9.24TWGDD248 pKa = 3.23HH249 pKa = 5.45GQAGYY254 pKa = 8.44LTDD257 pKa = 4.68LLSSSIEE264 pKa = 3.97LLSDD268 pKa = 3.44VAISGPVADD277 pKa = 5.75QLLKK281 pKa = 11.2YY282 pKa = 10.55NGSNWINYY290 pKa = 6.46TPSYY294 pKa = 9.98LEE296 pKa = 4.99SYY298 pKa = 10.29TEE300 pKa = 4.04TDD302 pKa = 3.08TLASITARR310 pKa = 11.84GASTTTTVTLTDD322 pKa = 4.38LNVSGNLNVLGTTTTNNVSTLNVTNNEE349 pKa = 3.42IVLNEE354 pKa = 3.98NQASGGLDD362 pKa = 2.79ALLTNEE368 pKa = 4.93RR369 pKa = 11.84GTDD372 pKa = 3.19ADD374 pKa = 5.36VSIKK378 pKa = 10.13WNEE381 pKa = 4.01TTDD384 pKa = 2.62RR385 pKa = 11.84WQFTNDD391 pKa = 2.19GSTYY395 pKa = 10.5YY396 pKa = 10.61NVAINASEE404 pKa = 4.09LTNDD408 pKa = 3.47AGYY411 pKa = 8.41LTTSSSINSLGDD423 pKa = 3.38VTISTPTSGDD433 pKa = 3.36VLSYY437 pKa = 11.13NGSDD441 pKa = 3.13WVNSPASITAKK452 pKa = 10.73ASISDD457 pKa = 4.12AAPSSPSPGDD467 pKa = 3.32MWWKK471 pKa = 10.25SDD473 pKa = 3.56EE474 pKa = 4.07GTMKK478 pKa = 10.13VWYY481 pKa = 9.67DD482 pKa = 4.39DD483 pKa = 4.18GNTAQWVDD491 pKa = 3.54ASPVGDD497 pKa = 3.59PFEE500 pKa = 4.24NVYY503 pKa = 11.15ASVAFFPGANVNTGAFAYY521 pKa = 10.44SEE523 pKa = 4.42ATGAMYY529 pKa = 10.71YY530 pKa = 10.4SNSVSWTSQRR540 pKa = 11.84LVTTNSSTSSDD551 pKa = 3.62FATLLANTQLTYY563 pKa = 10.73SVSAIDD569 pKa = 3.73YY570 pKa = 6.99TTGGTAEE577 pKa = 3.9YY578 pKa = 10.16NAARR582 pKa = 11.84KK583 pKa = 8.93IVRR586 pKa = 11.84LSDD589 pKa = 3.6SQGVTGDD596 pKa = 3.93IILTAGTGLSIVKK609 pKa = 9.98SNNEE613 pKa = 3.28ITFNNDD619 pKa = 2.5VVDD622 pKa = 3.82TTYY625 pKa = 11.04GISVEE630 pKa = 4.09ASSGANSILRR640 pKa = 11.84LTDD643 pKa = 3.29SQGVLDD649 pKa = 4.49DD650 pKa = 4.12VVFAGADD657 pKa = 3.51GLVVEE662 pKa = 4.87NTDD665 pKa = 3.22ANTFTFRR672 pKa = 11.84APDD675 pKa = 3.31ISSQFYY681 pKa = 10.15TDD683 pKa = 4.82EE684 pKa = 4.17EE685 pKa = 4.35AQDD688 pKa = 3.96AVATMFANGTHH699 pKa = 5.55TNITFTYY706 pKa = 10.57DD707 pKa = 3.17DD708 pKa = 4.25TNNSLSATAQAGGGGGGGTTYY729 pKa = 11.39DD730 pKa = 3.51LVGSNTNSNNAIITLLDD747 pKa = 3.77ANNNEE752 pKa = 4.49DD753 pKa = 3.99KK754 pKa = 11.16IEE756 pKa = 4.01IAGGGGTDD764 pKa = 4.13VSWDD768 pKa = 3.67GPNEE772 pKa = 4.41RR773 pKa = 11.84ITVSSTAPVQSDD785 pKa = 2.55WDD787 pKa = 3.82ATTGLAQILNKK798 pKa = 10.04PSIPSAYY805 pKa = 10.13ALPTAAAGTLGGIKK819 pKa = 10.14VGANLSIDD827 pKa = 3.41GDD829 pKa = 4.59GILSANAGGYY839 pKa = 6.9TLPAADD845 pKa = 3.84ATSLGGIKK853 pKa = 10.15VGSGLSIDD861 pKa = 3.98GNGVLTATGGSSVPSIQDD879 pKa = 3.24LSGTTASLAADD890 pKa = 3.54QSAEE894 pKa = 4.1LNITGYY900 pKa = 10.39KK901 pKa = 9.96AYY903 pKa = 10.93SLFKK907 pKa = 10.12VTTDD911 pKa = 2.8AEE913 pKa = 4.11AWVRR917 pKa = 11.84VYY919 pKa = 11.33VDD921 pKa = 3.58DD922 pKa = 5.58ASRR925 pKa = 11.84DD926 pKa = 3.42ADD928 pKa = 3.55NTRR931 pKa = 11.84SEE933 pKa = 5.02GEE935 pKa = 4.23DD936 pKa = 3.44PTPGSGVISEE946 pKa = 4.3VRR948 pKa = 11.84TSGAEE953 pKa = 3.87SVLISPGIMGFNNDD967 pKa = 3.48SPRR970 pKa = 11.84TDD972 pKa = 3.49TIYY975 pKa = 11.08LSVTNRR981 pKa = 11.84SGSATTITVTLTALQIGEE999 pKa = 4.14

Molecular weight: 103.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3FGA2|A0A0E3FGA2_9CAUD Plastocyanine OS=Synechococcus phage ACG-2014i OX=1493513 GN=Syn7803US120_95 PE=3 SV=1
MM1 pKa = 7.39AKK3 pKa = 10.09SRR5 pKa = 11.84VGLSGAEE12 pKa = 4.21TIEE15 pKa = 4.65SIPKK19 pKa = 8.64RR20 pKa = 11.84TRR22 pKa = 11.84QGRR25 pKa = 11.84GKK27 pKa = 7.68HH28 pKa = 4.65TKK30 pKa = 8.57YY31 pKa = 9.26TATSRR36 pKa = 11.84NGAKK40 pKa = 9.61KK41 pKa = 10.28RR42 pKa = 11.84YY43 pKa = 8.62RR44 pKa = 11.84GQGKK48 pKa = 9.26GG49 pKa = 3.22

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

212

0

212

60288

38

4102

284.4

31.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.738 ± 0.257

0.841 ± 0.082

6.688 ± 0.121

5.787 ± 0.278

4.296 ± 0.113

8.265 ± 0.381

1.358 ± 0.124

6.515 ± 0.18

5.319 ± 0.343

7.083 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.939 ± 0.201

6.17 ± 0.19

3.956 ± 0.125

3.531 ± 0.101

3.812 ± 0.119

7.681 ± 0.274

7.862 ± 0.32

6.726 ± 0.176

1.148 ± 0.083

4.279 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski