Microbacterium pygmaeum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3412 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7WS51|A0A1G7WS51_9MICO Putative adhesin OS=Microbacterium pygmaeum OX=370764 GN=SAMN04489810_1180 PE=4 SV=1
MM1 pKa = 7.34IRR3 pKa = 11.84WKK5 pKa = 10.39KK6 pKa = 9.6AAAAAAIAVTAALALSSCAGGATDD30 pKa = 4.8GGGEE34 pKa = 4.23GGSGGTLTLGAIAAPTTFDD53 pKa = 3.75PAGSEE58 pKa = 3.78WGNRR62 pKa = 11.84SPFYY66 pKa = 10.45QAVFDD71 pKa = 4.24TLLLATPEE79 pKa = 4.18GTIEE83 pKa = 3.82PWLATEE89 pKa = 4.74WSYY92 pKa = 12.27NDD94 pKa = 4.13DD95 pKa = 3.76NTVLTLTIRR104 pKa = 11.84DD105 pKa = 4.07DD106 pKa = 3.62VTFTDD111 pKa = 5.55GSALTADD118 pKa = 3.61VVVGNLQRR126 pKa = 11.84FKK128 pKa = 11.28DD129 pKa = 3.91GTSPDD134 pKa = 3.15AGYY137 pKa = 9.51FAGVASFEE145 pKa = 4.52APDD148 pKa = 3.76DD149 pKa = 3.88TTVVITLSAPDD160 pKa = 4.25PAMLDD165 pKa = 3.53YY166 pKa = 10.14LTRR169 pKa = 11.84DD170 pKa = 3.42PGLVGAEE177 pKa = 4.07ANFDD181 pKa = 3.85NPDD184 pKa = 3.44AATTPIGSGPYY195 pKa = 10.23VLDD198 pKa = 3.31TAATVTGTTYY208 pKa = 11.02AYY210 pKa = 8.09TKK212 pKa = 10.91NPDD215 pKa = 2.94YY216 pKa = 10.7WNPDD220 pKa = 2.95VQHH223 pKa = 6.58YY224 pKa = 10.47DD225 pKa = 3.17NLVINTLTDD234 pKa = 3.17PTAALNAIKK243 pKa = 10.33AGEE246 pKa = 4.02ANGVKK251 pKa = 10.06LANNDD256 pKa = 3.51ALDD259 pKa = 3.98EE260 pKa = 4.35VEE262 pKa = 4.29GAGWTVNANEE272 pKa = 5.21LDD274 pKa = 3.93FQGLLLLDD282 pKa = 4.02RR283 pKa = 11.84AGTMDD288 pKa = 4.61PALADD293 pKa = 3.49VKK295 pKa = 10.74VRR297 pKa = 11.84QAINYY302 pKa = 8.64AFDD305 pKa = 4.46RR306 pKa = 11.84EE307 pKa = 4.29GLLQATQFGNGTVTTQVFPATSDD330 pKa = 3.41AYY332 pKa = 10.76DD333 pKa = 4.06PEE335 pKa = 5.38LDD337 pKa = 3.44EE338 pKa = 5.23YY339 pKa = 10.33YY340 pKa = 10.49TYY342 pKa = 11.2DD343 pKa = 3.5PEE345 pKa = 4.43KK346 pKa = 10.79AKK348 pKa = 10.97SLLAEE353 pKa = 4.42AGYY356 pKa = 11.36ADD358 pKa = 4.69GLTISMPSVSVLGATTYY375 pKa = 10.89TLVAQQLADD384 pKa = 3.05IGITVEE390 pKa = 4.19QVDD393 pKa = 4.05VPMGNFIADD402 pKa = 4.6LLAPKK407 pKa = 10.34YY408 pKa = 8.63PASFMALEE416 pKa = 4.41QNPDD420 pKa = 2.7WQLIQFMIAPTAVFNPFKK438 pKa = 11.04YY439 pKa = 10.22SDD441 pKa = 3.94PQVDD445 pKa = 4.0EE446 pKa = 4.83YY447 pKa = 10.85IQEE450 pKa = 3.82IQYY453 pKa = 11.24GDD455 pKa = 4.11EE456 pKa = 3.94ATQASVAKK464 pKa = 9.68EE465 pKa = 3.68LNTYY469 pKa = 9.78IVEE472 pKa = 4.41QGWFAPFFRR481 pKa = 11.84VQGSVATDD489 pKa = 3.11ANTTVEE495 pKa = 4.19MLPTNAYY502 pKa = 8.08PAIYY506 pKa = 10.15DD507 pKa = 3.79FQPKK511 pKa = 7.63QQ512 pKa = 3.15

Molecular weight: 54.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7X088|A0A1G7X088_9MICO Demethylmenaquinone methyltransferase OS=Microbacterium pygmaeum OX=370764 GN=menG PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3412

0

3412

1117582

29

2029

327.5

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.54 ± 0.053

0.476 ± 0.009

6.407 ± 0.037

5.558 ± 0.039

3.182 ± 0.026

8.933 ± 0.034

1.959 ± 0.021

4.827 ± 0.029

1.782 ± 0.032

10.028 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.788 ± 0.016

1.896 ± 0.022

5.455 ± 0.036

2.756 ± 0.02

7.372 ± 0.046

5.682 ± 0.031

5.982 ± 0.031

8.824 ± 0.036

1.564 ± 0.018

1.99 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski