Escherichia phage Lambda_ev099

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lambdavirus; unclassified Lambdavirus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FTY9|A0A653FTY9_9CAUD Phage minor tail protein OS=Escherichia phage Lambda_ev099 OX=2742953 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 9.4HH3 pKa = 5.21TEE5 pKa = 3.58LRR7 pKa = 11.84AAVLDD12 pKa = 3.88ALEE15 pKa = 4.33KK16 pKa = 10.45HH17 pKa = 6.2DD18 pKa = 4.25TGATLFDD25 pKa = 3.55GRR27 pKa = 11.84PAVFDD32 pKa = 4.27EE33 pKa = 4.49EE34 pKa = 4.78DD35 pKa = 3.86FPAIAVYY42 pKa = 8.59LTGAEE47 pKa = 4.22YY48 pKa = 9.85TGEE51 pKa = 4.09EE52 pKa = 4.61LDD54 pKa = 3.6SDD56 pKa = 3.79TWQAEE61 pKa = 3.69LHH63 pKa = 6.35IEE65 pKa = 4.19VFLPAQVPDD74 pKa = 4.02SEE76 pKa = 4.66LDD78 pKa = 2.81AWMEE82 pKa = 3.93SRR84 pKa = 11.84IYY86 pKa = 10.51PVMSDD91 pKa = 3.25IPALSDD97 pKa = 5.02LITSMVASGYY107 pKa = 10.36DD108 pKa = 3.32YY109 pKa = 11.21RR110 pKa = 11.84RR111 pKa = 11.84DD112 pKa = 3.5DD113 pKa = 5.7DD114 pKa = 4.64AGLWSSADD122 pKa = 3.34LTYY125 pKa = 11.13VITYY129 pKa = 9.4EE130 pKa = 3.98MM131 pKa = 4.92

Molecular weight: 14.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FUF9|A0A653FUF9_9CAUD Phage tail fiber protein OS=Escherichia phage Lambda_ev099 OX=2742953 PE=4 SV=1
MM1 pKa = 7.52QYY3 pKa = 10.15PLHH6 pKa = 6.17VLPLSQRR13 pKa = 11.84ALLRR17 pKa = 11.84ILPYY21 pKa = 9.94FCSVLPLPHH30 pKa = 6.56CQQPLSPSWMPSWRR44 pKa = 11.84CPSLLTPVWSPHH56 pKa = 5.92LLKK59 pKa = 9.33QTQMMPMTPPNWRR72 pKa = 11.84TMRR75 pKa = 11.84QPFLRR80 pKa = 11.84ILRR83 pKa = 11.84WFPTLSRR90 pKa = 11.84SFRR93 pKa = 11.84MSSLRR98 pKa = 11.84KK99 pKa = 9.5QPSLLQWPVQWLRR112 pKa = 11.84QPSLWLLKK120 pKa = 10.72RR121 pKa = 11.84MMRR124 pKa = 11.84RR125 pKa = 11.84FLPIFRR131 pKa = 11.84RR132 pKa = 11.84RR133 pKa = 11.84WHH135 pKa = 5.88WLRR138 pKa = 11.84PARR141 pKa = 11.84HH142 pKa = 6.32LLTRR146 pKa = 11.84HH147 pKa = 4.92WQTTQPLFLSLPQLRR162 pKa = 11.84RR163 pKa = 11.84SVPLPSQKK171 pKa = 7.67TWRR174 pKa = 11.84SSRR177 pKa = 11.84TFLPPSRR184 pKa = 11.84NLLPPLPRR192 pKa = 11.84KK193 pKa = 9.28LRR195 pKa = 11.84YY196 pKa = 7.53WRR198 pKa = 11.84SRR200 pKa = 11.84MRR202 pKa = 11.84SFLMILPPLLKK213 pKa = 8.93PTHH216 pKa = 6.85PGLRR220 pKa = 11.84WQLLTLSS227 pKa = 4.08

Molecular weight: 27.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13757

43

1165

188.5

21.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.726 ± 0.574

1.185 ± 0.136

5.699 ± 0.258

6.055 ± 0.324

3.569 ± 0.216

6.949 ± 0.4

1.825 ± 0.169

5.11 ± 0.235

5.343 ± 0.298

7.967 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.799 ± 0.18

4.289 ± 0.268

4.005 ± 0.274

4.318 ± 0.287

6.586 ± 0.322

6.942 ± 0.295

6.273 ± 0.354

6.346 ± 0.353

1.926 ± 0.149

3.089 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski