Laribacter hongkongensis (strain HLHK9)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Chromobacteriaceae; Laribacter; Laribacter hongkongensis

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C1D5B9|C1D5B9_LARHH PhbF OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=phbF PE=4 SV=1
MM1 pKa = 7.17NAASEE6 pKa = 4.41MPLPLNFTDD15 pKa = 3.65SAVAKK20 pKa = 9.95VSEE23 pKa = 4.78LIAEE27 pKa = 4.44EE28 pKa = 4.47GNPDD32 pKa = 2.99LKK34 pKa = 11.14LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.94GFTFDD54 pKa = 4.81EE55 pKa = 4.53ITNEE59 pKa = 3.84DD60 pKa = 3.99DD61 pKa = 4.61AVMEE65 pKa = 4.42KK66 pKa = 10.96GGVTLLIDD74 pKa = 3.8PMSYY78 pKa = 10.31QYY80 pKa = 11.58LVGAEE85 pKa = 3.7IDD87 pKa = 3.88YY88 pKa = 11.15QEE90 pKa = 4.55SLEE93 pKa = 4.18GSQFVIKK100 pKa = 10.6NPNATSTCGCGSSFSVV116 pKa = 3.54

Molecular weight:
12.38 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C1D6L5|C1D6L5_LARHH Uncharacterized protein OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=LHK_01130 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVVKK11 pKa = 10.69RR12 pKa = 11.84KK13 pKa = 8.03RR14 pKa = 11.84THH16 pKa = 5.74GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 9.02TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.7GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight:
5.04 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3208

0

3208

919481

21

2414

286.6

31.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.017 ± 0.056

1.105 ± 0.018

5.42 ± 0.039

5.309 ± 0.045

3.521 ± 0.03

8.17 ± 0.052

2.255 ± 0.023

4.602 ± 0.034

3.137 ± 0.042

11.222 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.025

2.71 ± 0.031

5.177 ± 0.038

4.036 ± 0.034

7.068 ± 0.053

5.397 ± 0.035

5.102 ± 0.033

7.47 ± 0.044

1.476 ± 0.021

2.268 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski