Rahnella aquatilis (strain ATCC 33071 / DSM 4594 / JCM 1683 / NBRC 105701 / NCIMB 13365 / CIP 78.65)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Rahnella; Rahnella aquatilis

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2IQ67|H2IQ67_RAHAC Alkylated DNA repair protein OS=Rahnella aquatilis (strain ATCC 33071 / DSM 4594 / JCM 1683 / NBRC 105701 / NCIMB 13365 / CIP 78.65) OX=745277 GN=Rahaq2_3719 PE=4 SV=1
MM1 pKa = 7.33VFNKK5 pKa = 8.67ITTCLFLAFNLLPLADD21 pKa = 3.53VVYY24 pKa = 11.09AEE26 pKa = 4.68TLPACSGFQITEE38 pKa = 4.18SCSATPTTLEE48 pKa = 3.87NKK50 pKa = 8.54TLNTYY55 pKa = 8.29TVADD59 pKa = 3.99GAEE62 pKa = 4.2MQFSGLTVSGDD73 pKa = 3.27SGGAFHH79 pKa = 7.44LGNGSTLEE87 pKa = 4.31IIPEE91 pKa = 4.05NTSGYY96 pKa = 10.5SEE98 pKa = 4.14FTGNSSSGNGGAIFAKK114 pKa = 10.25EE115 pKa = 3.67NSTVYY120 pKa = 10.86LEE122 pKa = 4.09NTLFIDD128 pKa = 5.04NVAHH132 pKa = 7.51DD133 pKa = 4.6YY134 pKa = 11.44GGAIYY139 pKa = 8.71MYY141 pKa = 10.33GSNDD145 pKa = 3.5EE146 pKa = 4.39GDD148 pKa = 3.57VDD150 pKa = 4.13LTITNAVFSGNIASEE165 pKa = 4.41GKK167 pKa = 10.48GGAIYY172 pKa = 10.64SLNNKK177 pKa = 8.14VQLTNILFEE186 pKa = 4.53NNQAKK191 pKa = 9.74TNGNGSNGNGSGGAIDD207 pKa = 4.28ATDD210 pKa = 3.52NTTDD214 pKa = 2.84SHH216 pKa = 7.92GSMFITNSEE225 pKa = 4.07FTGNQAEE232 pKa = 4.53GQGGAIYY239 pKa = 9.7TNSASTPFFVDD250 pKa = 2.98INIDD254 pKa = 3.5EE255 pKa = 4.53NYY257 pKa = 10.21EE258 pKa = 4.15DD259 pKa = 4.14NNGVAYY265 pKa = 10.82YY266 pKa = 10.15HH267 pKa = 7.53DD268 pKa = 4.31NVADD272 pKa = 3.95NYY274 pKa = 11.55GNDD277 pKa = 3.52PDD279 pKa = 4.04AAGGGFMYY287 pKa = 10.53LGHH290 pKa = 6.27SEE292 pKa = 4.44ANFDD296 pKa = 3.35IAADD300 pKa = 3.57KK301 pKa = 10.64TLVIGNTANDD311 pKa = 3.86GAYY314 pKa = 10.52DD315 pKa = 4.16SIAGYY320 pKa = 9.84GAIFKK325 pKa = 10.38RR326 pKa = 11.84GAGEE330 pKa = 4.05LVLNADD336 pKa = 3.38NSGFTGVFDD345 pKa = 3.67ILEE348 pKa = 4.38GTVTLGRR355 pKa = 11.84DD356 pKa = 3.43DD357 pKa = 3.91TLVNVGDD364 pKa = 4.21TNCQSEE370 pKa = 4.5ADD372 pKa = 3.77TCHH375 pKa = 7.14GIVLGNANSMNDD387 pKa = 3.46KK388 pKa = 10.83AILNVGSTQQTFKK401 pKa = 10.85NAFMGHH407 pKa = 5.85EE408 pKa = 4.01NSTLNIDD415 pKa = 3.21AGGNVIVNSGYY426 pKa = 9.25MAGTTTGAGDD436 pKa = 3.51LTVAEE441 pKa = 4.69NGSFTLTGAQSMGLDD456 pKa = 3.16GDD458 pKa = 3.91IVVEE462 pKa = 4.11QNAVLSVQGDD472 pKa = 3.96AEE474 pKa = 4.31DD475 pKa = 4.4LAILQADD482 pKa = 3.94PQSIVLDD489 pKa = 3.92GGVLDD494 pKa = 5.08LSDD497 pKa = 3.29MSTFNGQTNQVNDD510 pKa = 3.64GLTITGTGGTVIGNDD525 pKa = 3.56DD526 pKa = 4.71LVTLGAGTDD535 pKa = 3.46YY536 pKa = 11.05HH537 pKa = 8.55IGDD540 pKa = 4.09GTSAADD546 pKa = 3.5GVYY549 pKa = 10.35VVIDD553 pKa = 3.85AGDD556 pKa = 3.67GRR558 pKa = 11.84VTLADD563 pKa = 3.34NNAYY567 pKa = 10.69LGTTQIASGTLQITDD582 pKa = 3.38NSQLGDD588 pKa = 3.29KK589 pKa = 10.37AYY591 pKa = 10.43NRR593 pKa = 11.84QVIFTDD599 pKa = 3.98PAQASTLEE607 pKa = 4.1VSAPADD613 pKa = 3.2GSGQVDD619 pKa = 3.61TTSAQTGKK627 pKa = 10.57ARR629 pKa = 11.84DD630 pKa = 3.32IEE632 pKa = 4.33MRR634 pKa = 11.84ASGTMQVDD642 pKa = 4.25DD643 pKa = 4.86GVTTQWGGLMADD655 pKa = 3.65STGAQADD662 pKa = 3.97VNSTFTKK669 pKa = 9.66TGNGTLILDD678 pKa = 4.21GTGSSHH684 pKa = 5.82SAVRR688 pKa = 11.84VEE690 pKa = 4.37QGTLQGGAEE699 pKa = 4.31NIIASASSLYY709 pKa = 11.07VGDD712 pKa = 3.57NATFEE717 pKa = 4.4TGLDD721 pKa = 3.51QQVQSLDD728 pKa = 3.54VASGGTIVISDD739 pKa = 4.04DD740 pKa = 3.63TQLMLMEE747 pKa = 4.76QDD749 pKa = 2.95TSAALDD755 pKa = 3.42ASLFADD761 pKa = 3.47TDD763 pKa = 3.84GTLVNATSGMSLQGTLNTNMTLDD786 pKa = 3.77ALTYY790 pKa = 10.59LPGVTVNGSLDD801 pKa = 3.55NSAGTASLQNGTAGDD816 pKa = 3.98TLTVNGDD823 pKa = 3.41YY824 pKa = 10.78TGGGTVVLEE833 pKa = 4.3TEE835 pKa = 4.41MNGDD839 pKa = 3.5TSATDD844 pKa = 3.46QLILNGNTSGDD855 pKa = 3.35TALAIVPVSGIGQATQAGIKK875 pKa = 10.22VVDD878 pKa = 5.3FVADD882 pKa = 3.63PQGNNNAADD891 pKa = 3.59FHH893 pKa = 6.84LAGDD897 pKa = 3.59QQYY900 pKa = 10.68INMGAYY906 pKa = 9.94DD907 pKa = 4.0YY908 pKa = 11.82SLVQDD913 pKa = 3.6NQDD916 pKa = 2.57WYY918 pKa = 11.36LRR920 pKa = 11.84SQVVTPDD927 pKa = 3.72PQPEE931 pKa = 4.14PVPGGDD937 pKa = 4.56DD938 pKa = 3.56FTPVLNPKK946 pKa = 9.07MGSYY950 pKa = 9.73MNNLRR955 pKa = 11.84IANNVFSMTAQDD967 pKa = 3.87HH968 pKa = 6.67AGADD972 pKa = 3.65HH973 pKa = 7.8DD974 pKa = 4.29NLKK977 pKa = 10.7LRR979 pKa = 11.84VDD981 pKa = 4.04TARR984 pKa = 11.84DD985 pKa = 3.16SATFGNQIDD994 pKa = 4.03SHH996 pKa = 7.76NDD998 pKa = 2.85TSVAQLSANFLQHH1011 pKa = 6.37PAGNGEE1017 pKa = 4.2LLAGIVAGYY1026 pKa = 9.96SNNSGDD1032 pKa = 3.87STSVLTGRR1040 pKa = 11.84KK1041 pKa = 9.08SDD1043 pKa = 4.0NDD1045 pKa = 3.68SQGYY1049 pKa = 9.57AVGLTGAWYY1058 pKa = 10.32QDD1060 pKa = 3.36AQAHH1064 pKa = 4.91QGAYY1068 pKa = 10.02LDD1070 pKa = 3.91SWLQYY1075 pKa = 10.66AWFDD1079 pKa = 3.54NSVSEE1084 pKa = 5.06EE1085 pKa = 4.01DD1086 pKa = 3.55TGDD1089 pKa = 3.92DD1090 pKa = 3.68NYY1092 pKa = 11.08HH1093 pKa = 6.67ASGLLASLEE1102 pKa = 3.86GGYY1105 pKa = 10.26RR1106 pKa = 11.84FTLMQGNQWNWTLQPQAQVIYY1127 pKa = 10.54QGVKK1131 pKa = 10.06QKK1133 pKa = 11.25DD1134 pKa = 3.7FTSASQSKK1142 pKa = 7.7VTQTGDD1148 pKa = 3.72DD1149 pKa = 4.08NIQTRR1154 pKa = 11.84LGLRR1158 pKa = 11.84SEE1160 pKa = 4.14WDD1162 pKa = 3.0IHH1164 pKa = 5.98PVSTLHH1170 pKa = 6.41IKK1172 pKa = 10.56PFVEE1176 pKa = 3.34ANYY1179 pKa = 10.2RR1180 pKa = 11.84HH1181 pKa = 6.11NSEE1184 pKa = 4.09QTSLNVDD1191 pKa = 3.41GVNFTDD1197 pKa = 3.7NTAKK1201 pKa = 10.76DD1202 pKa = 3.62SAEE1205 pKa = 4.03LSTGISGNLGANTTVWGKK1223 pKa = 10.44VGQQEE1228 pKa = 4.36GSDD1231 pKa = 3.9DD1232 pKa = 3.89FSQTEE1237 pKa = 3.97ATVGVSVRR1245 pKa = 11.84WW1246 pKa = 3.4

Molecular weight: 129.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2IZ43|H2IZ43_RAHAC Uncharacterized protein OS=Rahnella aquatilis (strain ATCC 33071 / DSM 4594 / JCM 1683 / NBRC 105701 / NCIMB 13365 / CIP 78.65) OX=745277 GN=Rahaq2_1064 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.55GRR39 pKa = 11.84TRR41 pKa = 11.84LSASKK46 pKa = 10.74

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4841

0

4841

1570583

23

4595

324.4

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.661 ± 0.042

1.032 ± 0.013

5.26 ± 0.024

5.44 ± 0.03

3.927 ± 0.026

7.491 ± 0.035

2.239 ± 0.02

5.808 ± 0.034

4.291 ± 0.026

10.812 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.02

3.785 ± 0.031

4.559 ± 0.023

4.51 ± 0.034

5.39 ± 0.035

6.257 ± 0.03

5.483 ± 0.042

7.035 ± 0.029

1.417 ± 0.016

2.851 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski