Synechococcus phage ACG-2014g

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Acionnavirus; unclassified Acionnavirus

Average proteome isoelectric point is 5.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 216 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3FDK0|A0A0E3FDK0_9CAUD Tail tube monomer protein OS=Synechococcus phage ACG-2014g OX=1493512 GN=Syn7803US105_105 PE=4 SV=1
MM1 pKa = 7.48TKK3 pKa = 10.24QSLGLGTTANDD14 pKa = 3.41NTGDD18 pKa = 3.84TLRR21 pKa = 11.84VGGDD25 pKa = 3.53KK26 pKa = 11.01INDD29 pKa = 3.71NFDD32 pKa = 4.03EE33 pKa = 4.82IYY35 pKa = 10.69SAFGNGTNLTLNVSNAATNQVLKK58 pKa = 11.22YY59 pKa = 9.44NGTSFVPGDD68 pKa = 3.84LGLLTTALDD77 pKa = 3.77VNNNNITSSSNNNISLVPNGTGDD100 pKa = 3.65LRR102 pKa = 11.84IVAGSITSTFDD113 pKa = 3.4GDD115 pKa = 4.01DD116 pKa = 3.67GTVDD120 pKa = 4.32FPTKK124 pKa = 9.63IRR126 pKa = 11.84YY127 pKa = 8.58INEE130 pKa = 3.92YY131 pKa = 9.07TSLGVAPASATYY143 pKa = 9.04TGYY146 pKa = 10.88FFTVDD151 pKa = 3.71GDD153 pKa = 3.95DD154 pKa = 3.72NPYY157 pKa = 11.1VNINVTAGGVGDD169 pKa = 4.17TAAKK173 pKa = 10.03IATEE177 pKa = 3.97YY178 pKa = 11.26SSIDD182 pKa = 3.52FLNDD186 pKa = 2.49VDD188 pKa = 4.22TTTVAPANDD197 pKa = 3.68QVLKK201 pKa = 10.25WSASSSKK208 pKa = 9.93WIPQDD213 pKa = 3.6DD214 pKa = 4.14QSGLTALNLFQTVTADD230 pKa = 3.42TGSTTANSGTDD241 pKa = 3.27TLIIAGGTDD250 pKa = 3.11IDD252 pKa = 4.04TAIVGDD258 pKa = 3.97TVTVNFSGVVPSTLDD273 pKa = 3.13TLSNVDD279 pKa = 4.55LSSLVQGDD287 pKa = 3.64SFYY290 pKa = 11.45YY291 pKa = 10.72NGTNWVRR298 pKa = 11.84SQSPLTWFEE307 pKa = 4.05LGSNGFNHH315 pKa = 6.09FTFSGAGFPVTQDD328 pKa = 3.17DD329 pKa = 3.79PTIYY333 pKa = 10.22VYY335 pKa = 10.71RR336 pKa = 11.84GFTYY340 pKa = 10.8AFDD343 pKa = 3.88NSSNGASHH351 pKa = 7.92PLRR354 pKa = 11.84IQSTTGLSGNPYY366 pKa = 9.38TVGQSGNGTSVLYY379 pKa = 7.98WTVPMDD385 pKa = 4.32APTTLYY391 pKa = 9.62YY392 pKa = 10.59QCTVHH397 pKa = 7.08AAMAGTIVVVNN408 pKa = 4.25

Molecular weight: 42.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3FDC2|A0A0E3FDC2_9CAUD Uncharacterized protein OS=Synechococcus phage ACG-2014g OX=1493512 GN=Syn7803US105_86 PE=4 SV=1
MM1 pKa = 7.23ATRR4 pKa = 11.84SKK6 pKa = 10.92SLSGQNCIEE15 pKa = 4.17SQPKK19 pKa = 8.32KK20 pKa = 8.78TRR22 pKa = 11.84QGNGAHH28 pKa = 6.05TKK30 pKa = 9.57YY31 pKa = 10.82ASSSRR36 pKa = 11.84NNARR40 pKa = 11.84KK41 pKa = 9.87RR42 pKa = 11.84YY43 pKa = 8.59RR44 pKa = 11.84GQGKK48 pKa = 9.26GG49 pKa = 3.22

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

216

0

216

55649

31

7019

257.6

28.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.897 ± 0.219

0.895 ± 0.098

6.573 ± 0.142

6.178 ± 0.298

4.365 ± 0.117

7.923 ± 0.336

1.483 ± 0.126

6.417 ± 0.216

5.535 ± 0.416

7.211 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.014 ± 0.229

5.944 ± 0.169

3.912 ± 0.117

3.569 ± 0.115

3.998 ± 0.156

7.373 ± 0.26

7.619 ± 0.479

6.832 ± 0.197

1.096 ± 0.087

4.165 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski