Pteropus associated gemycircularvirus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus ptero4

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTS6|A0A140CTS6_9VIRU Replication-associated protein OS=Pteropus associated gemycircularvirus 4 OX=1985398 PE=3 SV=1
MM1 pKa = 7.84PSNGLFVNSRR11 pKa = 11.84YY12 pKa = 10.31KK13 pKa = 10.92LLTYY17 pKa = 8.98AQCGDD22 pKa = 4.68LDD24 pKa = 4.05PFDD27 pKa = 5.0IVDD30 pKa = 4.94LLSGLQGEE38 pKa = 5.05CIVGRR43 pKa = 11.84EE44 pKa = 4.05LHH46 pKa = 6.34SDD48 pKa = 3.64GGIHH52 pKa = 6.48LHH54 pKa = 5.85VFVDD58 pKa = 4.54FGRR61 pKa = 11.84KK62 pKa = 8.03FRR64 pKa = 11.84SRR66 pKa = 11.84SVGIFDD72 pKa = 3.91VGGRR76 pKa = 11.84HH77 pKa = 6.0PNVVASWGTPEE88 pKa = 4.46EE89 pKa = 4.94GYY91 pKa = 10.93DD92 pKa = 3.63YY93 pKa = 10.79AIKK96 pKa = 10.96DD97 pKa = 3.59GDD99 pKa = 4.01VVAGGLEE106 pKa = 3.99RR107 pKa = 11.84PTPRR111 pKa = 11.84EE112 pKa = 3.63SRR114 pKa = 11.84AGNGSAAAKK123 pKa = 8.14WATIADD129 pKa = 3.82ADD131 pKa = 3.94DD132 pKa = 4.26RR133 pKa = 11.84EE134 pKa = 4.42QFFEE138 pKa = 4.51LVKK141 pKa = 11.14SLDD144 pKa = 3.21PKK146 pKa = 10.36TFVTRR151 pKa = 11.84LQDD154 pKa = 3.6LQRR157 pKa = 11.84FADD160 pKa = 3.36WKK162 pKa = 10.48FRR164 pKa = 11.84SDD166 pKa = 3.78PEE168 pKa = 4.52PYY170 pKa = 8.68ITPNNIHH177 pKa = 6.38FPDD180 pKa = 5.06DD181 pKa = 3.8GTDD184 pKa = 4.46GRR186 pKa = 11.84AEE188 pKa = 4.0WVRR191 pKa = 11.84DD192 pKa = 3.54NLVLDD197 pKa = 4.44WEE199 pKa = 4.41NHH201 pKa = 4.33SS202 pKa = 3.92

Molecular weight: 22.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTS5|A0A140CTS5_9VIRU RepA OS=Pteropus associated gemycircularvirus 4 OX=1985398 PE=3 SV=1
MM1 pKa = 7.23PRR3 pKa = 11.84YY4 pKa = 8.63YY5 pKa = 11.12AKK7 pKa = 9.51TRR9 pKa = 11.84RR10 pKa = 11.84FRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84VIGRR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 10.06SSLTGSRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84ITGAKK35 pKa = 8.42RR36 pKa = 11.84RR37 pKa = 11.84SYY39 pKa = 10.34GKK41 pKa = 9.39KK42 pKa = 8.28RR43 pKa = 11.84QMMTTRR49 pKa = 11.84RR50 pKa = 11.84ILNRR54 pKa = 11.84TSTKK58 pKa = 10.36KK59 pKa = 10.23RR60 pKa = 11.84DD61 pKa = 3.58TMLGATTFPPLPEE74 pKa = 4.1NVGALTVTATVPAVLVWCATARR96 pKa = 11.84NLQQEE101 pKa = 4.5ANALDD106 pKa = 4.64TIWSSAQRR114 pKa = 11.84TKK116 pKa = 9.82STCFIRR122 pKa = 11.84GLKK125 pKa = 8.16EE126 pKa = 3.82TITIRR131 pKa = 11.84TNSSAAWRR139 pKa = 11.84WRR141 pKa = 11.84RR142 pKa = 11.84IAFRR146 pKa = 11.84YY147 pKa = 9.73KK148 pKa = 10.36GLLPTGEE155 pKa = 4.06DD156 pKa = 2.86TFARR160 pKa = 11.84FFNEE164 pKa = 3.05ITEE167 pKa = 4.26ANRR170 pKa = 11.84VRR172 pKa = 11.84IMRR175 pKa = 11.84PVTALPFSIVSPLYY189 pKa = 9.2AHH191 pKa = 7.75IFRR194 pKa = 11.84GFGINDD200 pKa = 4.04LSANPQDD207 pKa = 3.4WVDD210 pKa = 4.82PITAPVDD217 pKa = 3.43TDD219 pKa = 4.83RR220 pKa = 11.84IAPLYY225 pKa = 10.3DD226 pKa = 2.77KK227 pKa = 10.8VVRR230 pKa = 11.84ITSGNDD236 pKa = 2.67AGVANTYY243 pKa = 10.2RR244 pKa = 11.84IWHH247 pKa = 6.23PVNKK251 pKa = 9.73NLRR254 pKa = 11.84YY255 pKa = 10.02NDD257 pKa = 3.69TEE259 pKa = 4.2FGGGFSSSPLSTEE272 pKa = 3.8GRR274 pKa = 11.84PGGGDD279 pKa = 3.64FYY281 pKa = 11.42VIDD284 pKa = 4.77IIIGNSSDD292 pKa = 4.51PGDD295 pKa = 4.06LLDD298 pKa = 4.35WLPTSTLYY306 pKa = 9.73WHH308 pKa = 7.06EE309 pKa = 4.2KK310 pKa = 9.32

Molecular weight: 35.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

829

202

317

276.3

31.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.393 ± 0.561

1.327 ± 0.442

8.203 ± 1.553

4.101 ± 0.652

5.549 ± 0.682

8.806 ± 0.843

2.292 ± 0.652

5.066 ± 0.843

4.101 ± 0.242

7.358 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.206 ± 0.282

4.584 ± 0.233

5.187 ± 0.39

2.533 ± 0.507

8.444 ± 1.756

6.393 ± 0.212

6.273 ± 1.993

6.514 ± 0.669

2.774 ± 0.19

2.895 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski