Amycolatopsis rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A558BTV3|A0A558BTV3_9PSEU Trk system potassium uptake protein TrkA OS=Amycolatopsis rhizosphaerae OX=2053003 GN=FNH05_23540 PE=4 SV=1
MM1 pKa = 7.75WIDD4 pKa = 3.51DD5 pKa = 3.7TGGADD10 pKa = 3.21TADD13 pKa = 3.76AGHH16 pKa = 6.53EE17 pKa = 4.27GEE19 pKa = 4.86MEE21 pKa = 3.97ISVDD25 pKa = 3.13GHH27 pKa = 6.38NYY29 pKa = 7.65TAEE32 pKa = 3.89EE33 pKa = 4.31NYY35 pKa = 10.74DD36 pKa = 3.9LNHH39 pKa = 7.19DD40 pKa = 4.17GVNDD44 pKa = 3.98TVKK47 pKa = 10.15MEE49 pKa = 4.38NSDD52 pKa = 3.75GTITAYY58 pKa = 10.96VDD60 pKa = 3.3TDD62 pKa = 3.71GDD64 pKa = 3.85GHH66 pKa = 8.01ADD68 pKa = 3.72EE69 pKa = 5.48YY70 pKa = 11.66VHH72 pKa = 7.21TDD74 pKa = 2.96TQGNVVEE81 pKa = 4.56MARR84 pKa = 11.84FDD86 pKa = 3.85ASTGTWVDD94 pKa = 3.55DD95 pKa = 3.94HH96 pKa = 6.91DD97 pKa = 4.08QSGGGSHH104 pKa = 6.59TPDD107 pKa = 3.23SQAGHH112 pKa = 6.16QGDD115 pKa = 3.65IMVDD119 pKa = 3.84TPQGTVDD126 pKa = 4.14AGHH129 pKa = 6.48ATIDD133 pKa = 3.99SNHH136 pKa = 7.29DD137 pKa = 3.43GTPDD141 pKa = 3.37TVQVTDD147 pKa = 3.76EE148 pKa = 4.24QGDD151 pKa = 4.13TILFTDD157 pKa = 3.82TNGDD161 pKa = 3.24GNADD165 pKa = 3.68VEE167 pKa = 4.99TVVTPSGEE175 pKa = 3.88HH176 pKa = 5.19HH177 pKa = 6.72TYY179 pKa = 10.28EE180 pKa = 4.26HH181 pKa = 6.78TGPGQWTEE189 pKa = 4.08TGGGGHH195 pKa = 6.4GVAAEE200 pKa = 4.32VPADD204 pKa = 4.35SDD206 pKa = 3.87QLWGGGHH213 pKa = 5.92DD214 pKa = 3.54TVVEE218 pKa = 4.51GVAKK222 pKa = 9.87IDD224 pKa = 3.77SVTGQWISQNN234 pKa = 3.38

Molecular weight: 24.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A558CDV5|A0A558CDV5_9PSEU Uncharacterized protein OS=Amycolatopsis rhizosphaerae OX=2053003 GN=FNH05_19650 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.68LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84MQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.69QGKK33 pKa = 8.53

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7256

0

7256

2237112

26

3931

308.3

33.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.342 ± 0.042

0.754 ± 0.009

5.652 ± 0.025

5.795 ± 0.033

2.93 ± 0.018

9.354 ± 0.029

2.278 ± 0.014

3.439 ± 0.019

1.964 ± 0.019

10.817 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.66 ± 0.011

1.851 ± 0.015

5.971 ± 0.027

2.782 ± 0.018

8.256 ± 0.034

4.994 ± 0.021

5.944 ± 0.028

8.667 ± 0.031

1.513 ± 0.012

2.039 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski