Denitrovibrio acetiphilus (strain DSM 12809 / NBRC 114555 / N2460)

Taxonomy: cellular organisms; Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Deferribacteraceae; Denitrovibrio; Denitrovibrio acetiphilus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2901 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4H2R2|D4H2R2_DENA2 DUF374 domain-containing protein OS=Denitrovibrio acetiphilus (strain DSM 12809 / NBRC 114555 / N2460) OX=522772 GN=Dacet_0323 PE=4 SV=1
MM1 pKa = 6.99MRR3 pKa = 11.84SLYY6 pKa = 10.63SAISGLSVNQQAMDD20 pKa = 3.5VLGNNISNVNTVGYY34 pKa = 10.08KK35 pKa = 9.53SGRR38 pKa = 11.84AVFEE42 pKa = 4.8DD43 pKa = 4.09MLSQTLVGSTAPSDD57 pKa = 3.69TLGGTNSTQVGLGTSLAGVDD77 pKa = 4.13TIFEE81 pKa = 4.39TGSMQSTSVNTDD93 pKa = 3.27LAIQGDD99 pKa = 4.42GFFVIKK105 pKa = 10.47GAGSSDD111 pKa = 3.56LLYY114 pKa = 10.73TRR116 pKa = 11.84AGDD119 pKa = 3.95FRR121 pKa = 11.84FDD123 pKa = 3.4TSGSLVTSSGFYY135 pKa = 8.23VQGWMVDD142 pKa = 3.34PDD144 pKa = 3.73TGEE147 pKa = 4.4LLTEE151 pKa = 4.63GTTEE155 pKa = 5.23DD156 pKa = 3.45IVLSSAYY163 pKa = 8.17QTAQAKK169 pKa = 6.81VTSEE173 pKa = 3.69ASLAGVLDD181 pKa = 3.84TDD183 pKa = 4.46ADD185 pKa = 4.11PSIVSYY191 pKa = 10.63PSLLHH196 pKa = 6.15YY197 pKa = 10.82ADD199 pKa = 4.08TGDD202 pKa = 5.44SIFQVYY208 pKa = 10.53SDD210 pKa = 5.19DD211 pKa = 3.97GANMDD216 pKa = 4.25LADD219 pKa = 4.09NEE221 pKa = 4.72SIKK224 pKa = 11.04VKK226 pKa = 10.7AHH228 pKa = 4.75GTGMTEE234 pKa = 3.11MGYY237 pKa = 9.69IYY239 pKa = 9.63NTSDD243 pKa = 3.24VNLDD247 pKa = 3.86LASDD251 pKa = 3.97PQLLVYY257 pKa = 10.56INSNPVTLEE266 pKa = 3.6YY267 pKa = 10.9GVDD270 pKa = 3.94FNTMAEE276 pKa = 4.16LANALEE282 pKa = 4.46TQLNTAAGAADD293 pKa = 4.14FSVAVVDD300 pKa = 3.7GTINVTRR307 pKa = 11.84NTVVGANVSIDD318 pKa = 3.8SFSGSPLLAVAMSDD332 pKa = 3.45LASVYY337 pKa = 11.09DD338 pKa = 3.98DD339 pKa = 5.12AGDD342 pKa = 3.77TKK344 pKa = 11.16SSDD347 pKa = 2.83EE348 pKa = 4.48MYY350 pKa = 10.81YY351 pKa = 10.54EE352 pKa = 4.08SQIYY356 pKa = 9.95AGRR359 pKa = 11.84DD360 pKa = 3.43FTTLTEE366 pKa = 3.93LASAIEE372 pKa = 3.96EE373 pKa = 4.68AIDD376 pKa = 3.88GNVIPTDD383 pKa = 3.94EE384 pKa = 4.53FNVTYY389 pKa = 10.71DD390 pKa = 3.13SATGQFNYY398 pKa = 10.55VIDD401 pKa = 3.86NTLNSALGTLRR412 pKa = 11.84LSGFSLDD419 pKa = 3.4KK420 pKa = 10.71AYY422 pKa = 11.14SGTVFEE428 pKa = 4.92NNIVPAGAVIEE439 pKa = 4.31AVYY442 pKa = 8.78GTTDD446 pKa = 3.37TEE448 pKa = 4.33LSNTFLRR455 pKa = 11.84TAQDD459 pKa = 3.9DD460 pKa = 4.17DD461 pKa = 4.38PLTEE465 pKa = 5.0LFTSSGDD472 pKa = 3.93SLGLDD477 pKa = 3.28ATAILQFSAGVAGEE491 pKa = 4.14NLAGTATIPAATSTLDD507 pKa = 3.66DD508 pKa = 3.99LRR510 pKa = 11.84EE511 pKa = 4.27AIAAYY516 pKa = 9.04MGYY519 pKa = 10.83DD520 pKa = 3.25SATEE524 pKa = 4.02DD525 pKa = 5.2DD526 pKa = 4.51VLQHH530 pKa = 6.54IASLDD535 pKa = 3.74KK536 pKa = 11.45NSGKK540 pKa = 10.12LQITGEE546 pKa = 4.14KK547 pKa = 10.27GSPNEE552 pKa = 3.38IDD554 pKa = 3.5FVKK557 pKa = 10.91FEE559 pKa = 4.44VVGSGTYY566 pKa = 9.0DD567 pKa = 3.07TFYY570 pKa = 11.09DD571 pKa = 3.9YY572 pKa = 11.81YY573 pKa = 10.79EE574 pKa = 4.55YY575 pKa = 10.6NTVQEE580 pKa = 4.52AKK582 pKa = 10.46GGTLTTSQTIYY593 pKa = 10.49DD594 pKa = 4.01AQGNAHH600 pKa = 5.13TVQYY604 pKa = 9.86NFEE607 pKa = 4.36MSDD610 pKa = 3.37SLDD613 pKa = 3.87NVWKK617 pKa = 9.21LTVSTPDD624 pKa = 3.47DD625 pKa = 3.66GAEE628 pKa = 3.59ISFNEE633 pKa = 4.1TTGNQVYY640 pKa = 7.3MHH642 pKa = 6.68FNANGSFNYY651 pKa = 8.53LTTEE655 pKa = 3.73SGQRR659 pKa = 11.84IASLSMNYY667 pKa = 8.41DD668 pKa = 3.13TGGGAGVVSDD678 pKa = 4.44IEE680 pKa = 4.26MDD682 pKa = 3.47LGTAGKK688 pKa = 10.16FDD690 pKa = 5.12GIYY693 pKa = 10.12ISSDD697 pKa = 2.93DD698 pKa = 4.21SGITKK703 pKa = 10.16AAQDD707 pKa = 4.12GYY709 pKa = 10.92ATGLLEE715 pKa = 4.19STLFNSNGEE724 pKa = 4.02IIGYY728 pKa = 6.03YY729 pKa = 10.2TNGQVATIAQIATATFTNPGGLTKK753 pKa = 10.76VGDD756 pKa = 3.62TTFQKK761 pKa = 10.67SSNSGDD767 pKa = 3.63PAIGRR772 pKa = 11.84PGTGFRR778 pKa = 11.84GKK780 pKa = 9.87VAAEE784 pKa = 3.75TLEE787 pKa = 4.0NSNVDD792 pKa = 3.51LSTEE796 pKa = 4.12FVNMITTQRR805 pKa = 11.84GFQANSRR812 pKa = 11.84VITTSDD818 pKa = 3.03EE819 pKa = 4.06MLQEE823 pKa = 4.04LMSLKK828 pKa = 10.43RR829 pKa = 3.65

Molecular weight: 87.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4H199|D4H199_DENA2 Short-chain dehydrogenase/reductase SDR OS=Denitrovibrio acetiphilus (strain DSM 12809 / NBRC 114555 / N2460) OX=522772 GN=Dacet_0041 PE=4 SV=1
MM1 pKa = 7.38AQMLTMKK8 pKa = 10.31KK9 pKa = 10.04PSNTKK14 pKa = 9.75RR15 pKa = 11.84KK16 pKa = 8.87RR17 pKa = 11.84KK18 pKa = 9.05HH19 pKa = 4.73GFRR22 pKa = 11.84ARR24 pKa = 11.84MKK26 pKa = 9.83TRR28 pKa = 11.84GGKK31 pKa = 9.28LAIARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 9.74GRR42 pKa = 11.84KK43 pKa = 8.88RR44 pKa = 11.84LTVV47 pKa = 3.11

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2901

0

2901

946645

32

3226

326.3

36.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.345 ± 0.044

1.208 ± 0.019

6.024 ± 0.051

6.95 ± 0.047

4.643 ± 0.035

6.894 ± 0.042

1.834 ± 0.022

7.607 ± 0.042

7.203 ± 0.051

8.931 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.028 ± 0.022

4.568 ± 0.037

3.342 ± 0.029

2.649 ± 0.024

4.218 ± 0.033

6.598 ± 0.04

5.362 ± 0.044

7.049 ± 0.048

0.746 ± 0.015

3.801 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski